Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,437,272 T→C 8.5% I194V (ATC→GTC)  pdxB ← erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,437,2720TC 8.5% 130.3 / 3.6 47I194V (ATC→GTC) pdxBerythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (22/21);  new base C (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

GACGTTGAGTTCCGGTTCGCCTTCCCAGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCA  >  NC_000913/2437126‑2437419
                                                                                                                                                  |                                                                                                                                                   
gACGTTGAGTTCCGGTTCGCCTTCCCATACATCCAGCACTACGCTTAACTTATGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGAt                                                                                                                                                     <  7:235553/147‑1 (MQ=255)
     tGAGTTCCGGTTCGCCTTCCCAGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGtttt                                                                                                                                              <  6:136698/149‑1 (MQ=255)
       aGTTCCGGTTCGCCTTCCCAGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTc                                                                                                                                            <  6:91519/149‑1 (MQ=255)
         ttCCGGTTCGCCTTCCCAGACATCCAACACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCAt                                                                                                                                          <  4:208260/149‑1 (MQ=255)
           ccGGTTCGCCTTCCCAGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATcc                                                                                                                                        >  7:29352/1‑149 (MQ=255)
               ttCGCCTTCCCAGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCa                                                                                                                                    <  3:34035/149‑1 (MQ=255)
                   ccTTCCCAGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGATTCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATg                                                                                                                                >  5:83511/1‑149 (MQ=255)
                          aGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGtt                                                                                                                         >  1:56906/1‑149 (MQ=255)
                          aGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGACCAGttt                                                                                                                                               <  8:136685/127‑1 (MQ=255)
                          aGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGACCAGttt                                                                                                                                               >  7:136685/1‑127 (MQ=255)
                            aCATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGtttt                                                                                                                       <  4:5930/149‑1 (MQ=255)
                            aCATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGtttt                                                                                                                       <  2:31788/149‑1 (MQ=255)
                                 cAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTAc                                                                                                                   <  5:114418/148‑1 (MQ=255)
                                     aCTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGAc                                                                                                               >  1:246132/1‑148 (MQ=255)
                                       tACGCTTAACTTCTGGCTTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCAt                                                                                                            >  2:97748/1‑149 (MQ=255)
                                         cGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCt                                                                                                          <  7:186013/149‑1 (MQ=255)
                                                cTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTaaa                                                                                                     >  4:90735/1‑147 (MQ=255)
                                                 ttCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAagag                                                                                                  >  7:15532/1‑149 (MQ=255)
                                                       ccTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCg                                                                                             >  3:17870/1‑148 (MQ=255)
                                                                      gTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGACCAGTTTTTCATCCGCCAGATGTAGCGtttt                                                                                                                       >  5:15521/1‑107 (MQ=255)
                                                                      gTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGACCAGTTTTTCATCCGCCAGATGTAGCGtttt                                                                                                                       <  6:15521/107‑1 (MQ=255)
                                                                         aGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATc                                                                          >  5:46959/1‑149 (MQ=255)
                                                                         aGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATc                                                                          >  5:198835/1‑149 (MQ=255)
                                                                            aaCGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCcgc                                                                       <  8:15532/149‑1 (MQ=255)
                                                                            aaCGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGGCAGAATATCcgc                                                                       <  1:220831/149‑1 (MQ=255)
                                                                                cAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCgcgcgc                                                                   >  8:1803/1‑149 (MQ=255)
                                                                                 aGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCt                                                                  >  6:211377/1‑149 (MQ=255)
                                                                                 aGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCt                                                                  >  2:92162/1‑149 (MQ=255)
                                                                                      tatCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGAc                                                              <  2:246132/148‑1 (MQ=255)
                                                                                        tCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTaa                                                           >  7:250229/1‑149 (MQ=255)
                                                                                          gacgacTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAAc                                                          <  8:36896/148‑1 (MQ=255)
                                                                                           acgacTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACt                                                         >  1:110290/1‑148 (MQ=255)
                                                                                           acgacTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTc                                                        <  7:140090/149‑1 (MQ=255)
                                                                                              acTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCTGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTATACAGTGGCGTATGGTAAGTCAGAATATCCGCGCGCAGGTCTTACTCATc                                                     <  2:234010/149‑1 (MQ=255)
                                                                                              acTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATc                                                     <  8:29352/149‑1 (MQ=255)
                                                                                                   gcCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCagc                                                 >  3:164121/1‑148 (MQ=255)
                                                                                                           aGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGc                                                                                                                                      >  7:31304/1‑55 (MQ=255)
                                                                                                               gTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCAc                                    >  5:203229/1‑149 (MQ=255)
                                                                                                                ttAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCAcc                                   <  7:1803/149‑1 (MQ=255)
                                                                                                                ttAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCAcc                                   <  2:56906/149‑1 (MQ=255)
                                                                                                                ttAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCAcc                                   <  3:90735/149‑1 (MQ=255)
                                                                                                                       gAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCAcc                                   <  2:69804/142‑1 (MQ=255)
                                                                                                                       gAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCAcc                                   >  1:69804/1‑142 (MQ=255)
                                                                                                                             cTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACgg                      >  7:230149/1‑149 (MQ=255)
                                                                                                                                              cGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGa       <  6:198835/147‑1 (MQ=255)
                                                                                                                                              cGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGAt      <  6:203229/148‑1 (MQ=255)
                                                                                                                                                 aTCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGc   >  4:111438/1‑148 (MQ=255)
                                                                                                                                                 aTCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGc   >  5:180257/1‑148 (MQ=255)
                                                                                                                                                  tCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCAAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCa  >  1:225909/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GACGTTGAGTTCCGGTTCGCCTTCCCAGACATCCAGCACTACGCTTAACTTCTGGCCTTCATTCAGGCAGGTCAGCAACGCAGTATTATCGACGACTGCGCCACGGCAGGCGTTAATCAGAATCGCTCCGGGCTTCAGGCTACGGATCAGTTTTTCATCCGCCAGATGTAGCGTTTTGTACGGACCATCTTTAAAGAGTGGCGTATGGAAAGTCAGAATATCCGCGCGCTGGACTAACTCATCCAGCGAGCGGAAATCACCCTCATCCCCACGGTCGGCGCGAGGCGGATCGCA  >  NC_000913/2437126‑2437419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑