Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,443,006 G→T 9.5% V415L (GTG→TTG)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,443,0060GT 9.5% 129.7 / 3.4 42V415L (GTG→TTG) mnmCfused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base G (18/19);  new base T (2/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

GTCAATGGATTTACCCGCAGAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACG  >  NC_000913/2442867‑2443147
                                                                                                                                           |                                                                                                                                             
gtCAATGGATTTACCCGCAGAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAAc                                                                                                                                      >  5:121496/1‑149 (MQ=255)
      ggATTTACCCGCAGAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATTTGCTGGAACTGgcg                                                                                                                                 >  1:3724/1‑148 (MQ=255)
      ggATTTACCCGCAGAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGAGACAAATTGCAGCGGCATTACTTATCCTCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATTTGCTGGAACTGgcg                                                                                                                                 >  5:227043/1‑148 (MQ=255)
        aTTTACCCGCAGAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCaa                                                                                                                              <  3:126445/149‑1 (MQ=255)
                   gAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGttt                                                                                                                   <  1:197544/149‑1 (MQ=255)
                    aaCTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTg                                                                                                                  >  4:117063/1‑149 (MQ=255)
                        ggCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTg                                                                                                                  >  6:149498/1‑145 (MQ=255)
                         gCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTGTCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGAt                                                                                                             >  7:111727/1‑149 (MQ=255)
                         gCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGAt                                                                                                             >  3:92439/1‑149 (MQ=255)
                             tAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttat                                                                                                         <  8:191838/149‑1 (MQ=255)
                                  gTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCa                                                                                                    <  6:223446/149‑1 (MQ=255)
                                  gTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCa                                                                                                    <  5:149498/149‑1 (MQ=255)
                                   ttGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCaa                                                                                                   >  4:1518/1‑149 (MQ=255)
                                      aGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAatat                                                                                                <  7:105141/149‑1 (MQ=255)
                                           aaTGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGtt                                                                                           <  3:117063/149‑1 (MQ=255)
                                                ggTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGa                                                                                      <  8:36650/149‑1 (MQ=255)
                                                      aCAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTAAAGAATTTAt                                                                                >  1:195792/1‑149 (MQ=255)
                                                          aTTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCg                                                                            >  8:204711/1‑149 (MQ=255)
                                                              cGGTCGTTGGGACAAATTGCAGCGTGATTACTTATTCGGTAGGTTGTTTGCTGTGCCCAGCAGAACTGACGCGTATTTTGCTGTAACTGGGGCAAGAGCAGGGTTTGCAGATTTTTTATCAATATCAGTTACAGAATTTATCCCGTaa                                                                         <  6:227043/148‑1 (MQ=255)
                                                              cGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATTTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTaa                                                                         <  2:3724/148‑1 (MQ=255)
                                                                       cGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttatta                                                                                                       <  7:58007/109‑1 (MQ=255)
                                                                       cGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttatta                                                                                                       >  8:58007/1‑109 (MQ=255)
                                                                        gACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATc                                                                                                     >  8:15707/1‑110 (MQ=255)
                                                                        gACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATc                                                                                                     <  7:15707/110‑1 (MQ=255)
                                                                         aCAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTgttg                                                             >  1:98158/1‑149 (MQ=255)
                                                                             aTTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTgttggttg                                                         >  3:79690/1‑149 (MQ=255)
                                                                                   gCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACGGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGa                                                                                      >  1:49333/1‑114 (MQ=255)
                                                                                   gCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACGGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGa                                                                                      <  2:49333/114‑1 (MQ=255)
                                                                                   gCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAt                                                   <  3:117920/149‑1 (MQ=255)
                                                                                   gCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAt                                                   <  7:204711/149‑1 (MQ=255)
                                                                                      gCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAtttt                                                <  2:98158/149‑1 (MQ=255)
                                                                                                tCCGCAAGGTGGTTGGCTGTTCCCAGCAGATATGACCCGTAATGTGCTGGAACTGGCGCAACATCAGGGGTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGAt                                       <  5:175274/148‑1 (MQ=255)
                                                                                                  cGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTTCAGAATTTACCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCag                                    >  3:126030/1‑149 (MQ=255)
                                                                                                       ggtggtTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCagcaag                                >  7:114698/1‑148 (MQ=255)
                                                                                                            ttGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTTGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAacac                          <  6:121496/149‑1 (MQ=255)
                                                                                                            ttGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAtttt                                                <  1:229541/127‑1 (MQ=255)
                                                                                                            ttGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAtttt                                                >  2:229541/1‑127 (MQ=255)
                                                                                                             tGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAacaca                         >  4:18503/1‑149 (MQ=255)
                                                                                                                       cagcagAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTg               <  4:79690/149‑1 (MQ=255)
                                                                                                                        agcagAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTgg              >  7:170177/1‑149 (MQ=255)
                                                                                                                               cTGACCCGTAATCTGATGGAACTGGCGCAACAGCAGGGATTGCAGATTTATTATCAAAATCAGTTACAGAATTTATCCCCTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGc       >  6:149882/1‑149 (MQ=255)
                                                                                                                                    ccGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACg  <  4:92439/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                             
GTCAATGGATTTACCCGCAGAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTATCCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACG  >  NC_000913/2442867‑2443147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑