Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,577,336 C→T 6.4% V348I (GTA→ATA)  maeB ← malic enzyme: putative oxidoreductase/phosphotransacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,577,3360CT 6.4% 196.3 / 2.3 62V348I (GTA→ATA) maeBmalic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base C (30/28);  new base T (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACCGCAGGAGCGATCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTC  >  NC_000913/2577188‑2577484
                                                                                                                                                    |                                                                                                                                                    
gCGACCGCAGGAGCGATCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTAc                                                                                                                                                      <  7:195177/149‑1 (MQ=255)
   aCCGCAGGAGCGATCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCTCCCGTAccgc                                                                                                                                                   >  6:213390/1‑149 (MQ=255)
    ccGCAGGAGCGATCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTAccgcc                                                                                                                                                  >  5:169629/1‑149 (MQ=255)
      gCAGGAGCGATCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACcgccgc                                                                                                                                                <  6:15638/149‑1 (MQ=255)
      gCAGGAGCGATCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCGCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACcgccgc                                                                                                                                                <  5:188988/149‑1 (MQ=255)
           aGCGATCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGtt                                                                                                                                           <  2:132134/149‑1 (MQ=255)
              gATCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCa                                                                                                                                        >  7:134595/1‑149 (MQ=255)
               aTCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCAt                                                                                                                                       >  3:10396/1‑149 (MQ=255)
                  ttAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCAtctc                                                                                                                                    >  8:162261/1‑149 (MQ=255)
                  ttAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCAtctc                                                                                                                                    >  2:192778/1‑149 (MQ=255)
                     aCGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCtt                                                                                                                                  <  4:162406/148‑1 (MQ=255)
                      cGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCg                                                                                                                                <  1:15303/149‑1 (MQ=255)
                       gATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGt                                                                                                                               >  4:65277/1‑149 (MQ=255)
                                   aTCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTATCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTg                                                                                                                   >  3:64908/1‑149 (MQ=255)
                                   aTCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTATCGCCGCCAGTTTCATCTCTTCGTTGATGGAGGTTg                                                                                                                   >  3:61836/1‑149 (MQ=255)
                                   aTCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTg                                                                                                                   >  4:23742/1‑149 (MQ=255)
                                       aaCGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCACATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTgcgc                                                                                                                >  7:17402/1‑148 (MQ=255)
                                                 gAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAg                                                                                                      <  3:65277/148‑1 (MQ=255)
                                                   atgatgTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAgcgc                                                                                                   >  6:152796/1‑149 (MQ=255)
                                                       tgTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCa                                                                                               <  8:134595/149‑1 (MQ=255)
                                                         tATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATGTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACg                                                                                             >  6:41928/1‑149 (MQ=255)
                                                             ccGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGaa                                                                                          <  1:192778/148‑1 (MQ=255)
                                                             ccGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCAGCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGaa                                                                                          <  5:59838/148‑1 (MQ=255)
                                                                gACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGAt                                                                                       >  3:29072/1‑148 (MQ=255)
                                                                   cAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAAc                                                                                   >  3:207499/1‑149 (MQ=255)
                                                                   cAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAAc                                                                                   >  6:35018/1‑149 (MQ=255)
                                                                   cAAAGCTCAGATCCTGATCGCCATACCCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAAc                                                                                   >  1:148277/1‑149 (MQ=255)
                                                                     aaGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACgg                                                                                 >  2:234509/1‑149 (MQ=255)
                                                                      aGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACggg                                                                                <  4:220820/149‑1 (MQ=255)
                                                                        cTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCAt                                                                                                                                       >  1:104196/1‑92 (MQ=255)
                                                                        cTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCAt                                                                                                                                       <  2:104196/92‑1 (MQ=255)
                                                                        cTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACggg                                                                                >  5:212878/1‑147 (MQ=255)
                                                                         tCAGCTCCTGATCGCCATACGCTGCAGCCACCACTTCGCTCTGGTCCGCCTGGGCGAGTTCTGCAATCGCCCGAACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGACCGGGCAg                                                                             >  2:97921/1‑149 (MQ=255)
                                                                         tCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAg                                                                             >  5:109202/1‑149 (MQ=255)
                                                                           aGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATGTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAg                                                                             <  5:41928/147‑1 (MQ=255)
                                                                                   aTCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGCACGGGAAGCCCAGGACgt                                                                   >  8:193824/1‑149 (MQ=255)
                                                                                    tCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACgtt                                                                  <  4:29072/149‑1 (MQ=255)
                                                                                      gCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACgttg                                                                 <  8:214777/148‑1 (MQ=255)
                                                                                      gCCATACGCTGAAGCCACCACTTCGCTATGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTTATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACgttgt                                                                <  6:169629/149‑1 (MQ=255)
                                                                                         aTACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCa                                                             <  5:126368/149‑1 (MQ=255)
                                                                                                 aaGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGtt                                                      <  8:84791/148‑1 (MQ=255)
                                                                                                  aGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTc                                                     <  5:152796/148‑1 (MQ=255)
                                                                                                      accacTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCaa                                                                                                             >  5:65191/1‑88 (MQ=255)
                                                                                                      accacTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCaa                                                                                                             <  6:65191/88‑1 (MQ=255)
                                                                                                          cTTCGCTCTGTTCCGCATCGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCa                                            <  8:117352/149‑1 (MQ=255)
                                                                                                           ttCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGt                                              >  1:101762/1‑146 (MQ=255)
                                                                                                              gCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAAc                                        <  4:10396/149‑1 (MQ=255)
                                                                                                                tctGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACAGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGa                                      >  6:42998/1‑149 (MQ=255)
                                                                                                                    ttCCGCATGGGCGAGTTCTGCAATCGCACGTATCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGAc                                     <  4:64908/146‑1 (MQ=255)
                                                                                                                    ttCCGCATGGGCGAGTTCTGCAATCGCACGTATCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGAc                                     <  4:61836/146‑1 (MQ=255)
                                                                                                                    ttCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGAcc                                    <  3:23742/147‑1 (MQ=255)
                                                                                                                    ttCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGAc                                     <  6:109202/146‑1 (MQ=255)
                                                                                                                       cGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTg                                <  7:40684/148‑1 (MQ=255)
                                                                                                                        gCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGc                               >  8:49343/1‑148 (MQ=255)
                                                                                                                             ggCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAatga                         <  1:234509/149‑1 (MQ=255)
                                                                                                                              gCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAatgat                        >  6:139029/1‑149 (MQ=255)
                                                                                                                              gCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAatgat                        >  2:84919/1‑149 (MQ=255)
                                                                                                                               cGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAatgatg                       <  7:49343/149‑1 (MQ=255)
                                                                                                                                gAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCa                                            <  7:28369/127‑1 (MQ=255)
                                                                                                                                gAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCa                                            >  8:28369/1‑127 (MQ=255)
                                                                                                                                      tGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCg                >  8:134681/1‑149 (MQ=255)
                                                                                                                                      tGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCg                >  2:122468/1‑149 (MQ=255)
                                                                                                                                      tGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCg                >  2:110310/1‑149 (MQ=255)
                                                                                                                                       gCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCg                >  7:85906/1‑148 (MQ=255)
                                                                                                                                                 gTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCActtct     <  2:101762/149‑1 (MQ=255)
                                                                                                                                                    ccgccgCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGATCGGGAAGCACAGGACGTTGTTCTCCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCCTCCGGACGCACTTCTTTc  >  6:224362/1‑149 (MQ=255)
                                                                                                                                                    ccgccgCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCttt   <  8:17402/148‑1 (MQ=255)
                                                                                                                                                    ccgccgCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTc  <  2:148277/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGACCGCAGGAGCGATCTTAACGATCAAGCGCGGATCAAACGGTTTTGGAATGATGTATTCCGGACCAAAGCTCAGATCCTGATCGCCATACGCTGAAGCCACCACTTCGCTCTGTTCCGCATGGGCGAGTTCTGCAATCGCACGTACCGCCGCCAGTTTCATCTCTTCGTTGATGGCGGTTGCGCCAACGTCCAGCGCGCCACGGAAGATGAACGGGAAGCACAGGACGTTGTTCACCTGGTTCGGATAGTCAGAACGACCGGTGCAAATGATGGCATCCGGACGCACTTCTTTC  >  NC_000913/2577188‑2577484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: