Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,669,513 C→T 7.3% P161L (CCG→CTG)  hcaE → 3‑phenylpropionate dioxygenase, large (alpha) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,669,5130CT 7.3% 164.2 / 2.8 55P161L (CCG→CTG) hcaE3‑phenylpropionate dioxygenase, large (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base C (27/24);  new base T (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

GGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGAC  >  NC_000913/2669368‑2669661
                                                                                                                                                 |                                                                                                                                                    
ggCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATTAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCggg                                                                                                                                                   >  3:213179/1‑149 (MQ=255)
   aTTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCt                                                                                                                                                >  5:88316/1‑149 (MQ=255)
     tAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGGTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGc                                                                                                                                              >  7:231605/1‑149 (MQ=255)
       aCGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAATGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCg                                                                                                                                             <  3:142441/148‑1 (MQ=255)
           cGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGAtt                                                                                                                                        <  7:49947/149‑1 (MQ=255)
             aGTTGATCGATGTACCGCTGGAACCTCCCGCCTACCCACAAGGGTTGGGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTAc                                                                                                                                      <  1:152648/149‑1 (MQ=255)
                  aTCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTgg                                                                                                                                  <  6:91827/148‑1 (MQ=255)
                  aTCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAACGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTggg                                                                                                                                 >  1:216967/1‑149 (MQ=255)
                               tGGATCCTCGCGCCTACCCACAAGGGTTGTGTATATCCCACTGGGGAATAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTgg                                                                                                                    <  3:82740/149‑1 (MQ=255)
                                 gAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGa                                                                                                                                          <  5:65743/125‑1 (MQ=255)
                                 gAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCAACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGa                                                                                                                                          >  6:65743/1‑125 (MQ=255)
                                  aaCCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTAt                                                                                                                 <  2:216967/149‑1 (MQ=255)
                                     cTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTg                                                                                                              >  4:164989/1‑149 (MQ=255)
                                     cTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTg                                                                                                              >  3:164798/1‑149 (MQ=255)
                                        gcgcCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGAt                                                                                                           <  6:88316/149‑1 (MQ=255)
                                                ccACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGTATACCAGCGCACCGGGACTGAGTGATTACCTGGGTGACATTGCATGTTATCTGGATGGCATgat                                                                                                   >  2:192537/1‑147 (MQ=255)
                                                  acaAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTg                                                                                                  >  1:122859/1‑148 (MQ=255)
                                                    aaGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGAt                                                                                               >  7:117954/1‑149 (MQ=255)
                                                     aGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGAtc                                                                                              <  1:247049/149‑1 (MQ=255)
                                                           gtgtAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTcgc                                                                                         <  4:164798/148‑1 (MQ=255)
                                                              tAACTCCCACTGGGTACTAAACGAAGTTCCTTGTGTGGTGAGTTATAAAGTGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGaa                                                                                      <  2:212349/148‑1 (MQ=255)
                                                                aaTCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAgg                                                                                    <  3:164989/148‑1 (MQ=255)
                                                                      aCTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCAcc                                                                             >  1:138718/1‑149 (MQ=255)
                                                                               tAAACGAAGTTCCTTGTGTTGAGATTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGTTGACATTGCCTGGTATGTGGATGGCATGCTGGCTCGTCGCTAAGGCGGCACCGAAATTGTc                                                                    >  4:22137/1‑149 (MQ=255)
                                                                                    gAAGTTCCTTGTGTGGCGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCGGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTcggcgg                                                               <  1:192537/149‑1 (MQ=255)
                                                                                    gAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGAt                                                                                                                                         <  2:10628/75‑1 (MQ=255)
                                                                                    gAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGAt                                                                                                                                         >  1:10628/1‑75 (MQ=255)
                                                                                    gAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTcggcgg                                                               >  8:224152/1‑149 (MQ=255)
                                                                                       gTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGgcggcg                                                             >  7:210188/1‑148 (MQ=255)
                                                                                          ccTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTcgc                                                                                         >  7:57438/1‑117 (MQ=255)
                                                                                          ccTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTcgc                                                                                         <  8:57438/117‑1 (MQ=255)
                                                                                            ttGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACTGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACaa                                                        >  2:116249/1‑148 (MQ=255)
                                                                                            ttGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACTGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACaa                                                        >  2:119564/1‑148 (MQ=255)
                                                                                              gtgtgGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGTATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGTCATTCTGGATAGTCGCGAAGGCGGCACCTAAATTGTCGGCGGCGTACaaaa                                                      <  3:22137/148‑1 (MQ=255)
                                                                                                 tgGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTgg                                                  >  1:234257/1‑149 (MQ=255)
                                                                                                 tgGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTgg                                                  <  2:122859/149‑1 (MQ=255)
                                                                                                   gagaGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTggg                                                 <  8:117954/148‑1 (MQ=255)
                                                                                                   gagaGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGt                                                <  4:86837/149‑1 (MQ=255)
                                                                                                    agagTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGt                                                >  7:110543/1‑148 (MQ=255)
                                                                                                       gTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATc                                            <  8:210188/149‑1 (MQ=255)
                                                                                                        ttATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCTCCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATc                                            >  5:192666/1‑148 (MQ=255)
                                                                                                          ataAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAAc                                         >  6:205194/1‑149 (MQ=255)
                                                                                                           taAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACt                                        >  8:9213/1‑149 (MQ=255)
                                                                                                                ggCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGACGGCGGCGTACAAAAGTGGGTGATCAACTGTAAc                                   >  3:57908/1‑149 (MQ=255)
                                                                                                                 gCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACt                                  >  2:186806/1‑149 (MQ=255)
                                                                                                                   tAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACaaaa                                                      <  4:154145/127‑1 (MQ=255)
                                                                                                                   tAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACaaaa                                                      >  3:154145/1‑127 (MQ=255)
                                                                                                                            gCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCg                       <  2:138718/149‑1 (MQ=255)
                                                                                                                              aaCTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGc                     >  4:92540/1‑149 (MQ=255)
                                                                                                                               aCTGGGATACCAGCGCACTGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCa                    <  1:119564/149‑1 (MQ=255)
                                                                                                                               aCTGGGATACCAGCGCACTGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCa                    <  1:116249/149‑1 (MQ=255)
                                                                                                                                   ggATACCAGCGCACCGGGCCTGCGTGATTACCTGGGGGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCaga                  <  4:213179/147‑1 (MQ=255)
                                                                                                                                    gATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCagag                 >  2:248361/1‑147 (MQ=255)
                                                                                                                                          aGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACt                                        >  2:28974/1‑118 (MQ=255)
                                                                                                                                          aGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACt                                        <  1:28974/118‑1 (MQ=255)
                                                                                                                                                 cGGGCCTGGGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGAc  <  3:92540/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCACAAGGGTTGTGTAAATCCCACTGGGGACTAAACGAAGTTCCTTGTGTGGAGAGTTATAAAGGGCTAATTTTTGGCAACTGGGATACCAGCGCACCGGGCCTGCGTGATTACCTGGGTGACATTGCCTGGTATCTGGATGGCATGCTGGATCGTCGCGAAGGCGGCACCGAAATTGTCGGCGGCGTACAAAAGTGGGTGATCAACTGTAACTGGAAATTCCCGGCAGAGCAGTTCGCCAGTGAC  >  NC_000913/2669368‑2669661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑