Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,860,614 A→T 6.8% D207E (GAT→GAA)  ygbI ← DeoR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,860,6140AT 6.8% 180.3 / 3.8 59D207E (GAT→GAA) ygbIDeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (27/28);  new base T (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

ATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTC  >  NC_000913/2860469‑2860761
                                                                                                                                                 |                                                                                                                                                   
attCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATca                                                                                                                                                  >  7:8877/1‑149 (MQ=255)
                  gCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAACCTCGCTgg                                                                                                                                >  3:139447/1‑149 (MQ=255)
                    aaaGATAGATCCTGCTTCACCAGCGCGCGCCTGGCACTCTCCGGCAAACAGTCGTCTGTAATAATCTGATCAAAATCGATTAATGGTACCGCCATCAATGTCGCCAACTGACCATTTTACGTCGCATCACATAACAAAACTCGCTgccc                                                                                                                              >  6:225017/1‑146 (MQ=255)
                       gagagaTCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATca                                                                                                                                                  <  1:39981/126‑1 (MQ=255)
                       gagagaTCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATca                                                                                                                                                  >  2:39981/1‑126 (MQ=255)
                            aaccTGCTTCACCAGCGCGCGACTGGCACTCTCAGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCATCCATCTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTAGCTGGCGGctggccc                                                                                                                      >  7:66208/3‑147 (MQ=255)
                            aTCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGctggcac                                                                                                                      >  6:169660/1‑149 (MQ=255)
                              ccTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGAc                                                                                                                                             <  7:132057/124‑1 (MQ=255)
                              ccTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGAc                                                                                                                                             >  8:132057/1‑124 (MQ=255)
                               cTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGctggcactgg                                                                                                                   >  3:56072/1‑149 (MQ=255)
                                   ttCACCAGCGCGCGACTGCCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATca                                                                                                                                                  >  6:232024/1‑114 (MQ=255)
                                   ttCACCAGCGCGCGACTGCCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATca                                                                                                                                                  <  5:232024/114‑1 (MQ=255)
                                    tCACCAGCGCGCGACTGGCACTCTCCTGCAAACCGTCGTCTGTAATAATCTGATCAAAATCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGAACATATTTCGTCGCATCACAGACCACACCTCTCTGGCGGCTGGCACTGGCAATc                                                                                                              >  2:76327/1‑149 (MQ=255)
                                     cACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATc                                                                                                              <  4:56072/148‑1 (MQ=255)
                                      aCCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGc                                                                                                            >  5:20727/1‑149 (MQ=255)
                                          gcgcgcGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGt                                                                                                        <  5:169660/149‑1 (MQ=255)
                                               cgACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCAc                                                                                                   <  6:183882/149‑1 (MQ=255)
                                                   tGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCg                                                                                                                                    >  8:224517/1‑112 (MQ=255)
                                                   tGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGt                                                                                                >  2:13383/1‑148 (MQ=255)
                                                     gCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGAc                                                                                             >  4:167765/1‑149 (MQ=255)
                                                     gCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGAc                                                                                             <  8:45991/149‑1 (MQ=255)
                                                     gCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGAc                                                                                             <  1:68737/149‑1 (MQ=255)
                                                       aCTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACtt                                                                                           <  7:90122/149‑1 (MQ=255)
                                                         tctcCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTa                                                                                         >  7:35674/1‑149 (MQ=255)
                                                         tctcCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTa                                                                                         >  4:64313/1‑149 (MQ=255)
                                                           tcCGGCAAACCGTCGTCTGTAATAATCTGAGCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTa                                                                                         <  5:225017/147‑1 (MQ=255)
                                                             cGGCAAACCGTTGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCTTCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCtt                                                                                     >  2:130527/1‑149 (MQ=255)
                                                             cGGCAAACCGTTGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCTTCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCtt                                                                                     >  2:126681/1‑149 (MQ=255)
                                                             cGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATc                                                                                       <  3:2221/147‑1 (MQ=255)
                                                              ggCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTc                                                                                    <  8:8877/149‑1 (MQ=255)
                                                                    ccGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTgg                                                                              <  6:20727/149‑1 (MQ=255)
                                                                       tcgtcTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCg                                                                            >  7:214763/1‑148 (MQ=255)
                                                                          tcTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGa                                                                        <  8:35674/149‑1 (MQ=255)
                                                                              taataaTCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCTTCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATc                                                                    <  1:126681/149‑1 (MQ=255)
                                                                              taataaTCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCTTCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATc                                                                    <  1:130527/149‑1 (MQ=255)
                                                                              taataaTCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATc                                                                    >  4:49335/1‑149 (MQ=255)
                                                                                ataatCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACGATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCCCCGTGACTTTATCTTCCGCTGGCGTAGAAATccc                                                                  <  2:224400/149‑1 (MQ=255)
                                                                                   atCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATccccc                                                                <  4:110112/148‑1 (MQ=255)
                                                                                   atCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATccccc                                                                <  3:49335/148‑1 (MQ=255)
                                                                                        aTCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCGGACCATATTTCGTCGCATCACAGCCCACCACTAGCTGGCGGCTGGCACTGGCAATCGCCCGATTCACCGTGCCTTTATCTGCCGCTGGCGTAGAAATCCCCCGccaac                                                          >  6:149452/1‑145 (MQ=255)
                                                                                             aCTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCAt                                                     >  7:29557/1‑149 (MQ=255)
                                                                                               tCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGa                                                   >  6:173904/1‑149 (MQ=255)
                                                                                                 gCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGAc                                                  <  1:13383/148‑1 (MQ=255)
                                                                                                   ttAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGAt                                               >  4:169389/1‑149 (MQ=255)
                                                                                                   ttAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGAt                                               >  4:13773/1‑149 (MQ=255)
                                                                                                     aaTGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATg                                              <  8:214763/148‑1 (MQ=255)
                                                                                                      aTGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCTTCGCATCACATACCAAAACTCGCTGGCGGCTGGAAGTGGCAATCGCCCGTTTCACCGTGAATTTTTCTTCCGCTGGCGTAGAAATCCCCCGCACTCTCCATGACGATGCa                                            <  2:216666/149‑1 (MQ=255)
                                                                                                      aTGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCa                                            <  3:167765/149‑1 (MQ=255)
                                                                                                          tAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGaaa                                        >  3:84840/1‑149 (MQ=255)
                                                                                                              gccagccaTGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATaa                                     >  7:202525/1‑148 (MQ=255)
                                                                                                                     aTGTCGCCACCTGACCATTTTTCTTAGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAAGCGACCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCtta                             <  4:139447/149‑3 (MQ=255)
                                                                                                                      tGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGat                            <  4:84840/149‑1 (MQ=255)
                                                                                                                          gCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGatcaat                        <  3:13773/149‑1 (MQ=255)
                                                                                                                              ccTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAatcatc                    <  8:249760/149‑1 (MQ=255)
                                                                                                                                  aCCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGc                >  6:89490/1‑149 (MQ=255)
                                                                                                                                     atatTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTgc             <  5:173904/149‑1 (MQ=255)
                                                                                                                                            gtcgCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCACCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCAt      >  7:202518/1‑149 (MQ=255)
                                                                                                                                                cATCACAGACCAAAACTCGCTGGCGGCTGGCACTTGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTc  <  7:123888/149‑1 (MQ=255)
                                                                                                                                                cATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTc  <  1:152475/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCATGTCGCCACCTGACCATATTTCGTCGCATCACAGACCAAAACTCGCTGGCGGCTGGCACTGGCAATCGCCCGTTTCACCGTGACTTTATCTTCCGCTGGCGTAGAAATCCCCCGCACACTCCATGACGATGCAGAAATAAAAGCCTGATCAATCATCAGGCTGCGCAGCATGGTC  >  NC_000913/2860469‑2860761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑