Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,892,345 A→G 7.1% E44E (GAA→GAG)  queD → 6‑pyruvoyl tetrahydrobiopterin synthase (PTPS)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,892,3450AG 7.1% 164.4 / 2.9 56E44E (GAA→GAG) queD6‑pyruvoyl tetrahydrobiopterin synthase (PTPS)
Reads supporting (aligned to +/- strand):  ref base A (24/28);  new base G (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

TAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGG  >  NC_000913/2892204‑2892492
                                                                                                                                             |                                                                                                                                                   
tAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGAt                                                                                                                                               <  4:167217/148‑1 (MQ=255)
 agagAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATcc                                                                                                                                             >  2:174147/1‑149 (MQ=255)
      aaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATa                                                                                                                                        <  7:171460/149‑1 (MQ=255)
       aTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGACATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATAc                                                                                                                                       >  3:203954/1‑149 (MQ=255)
              tgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTgg                                                                                                                                <  7:32253/149‑1 (MQ=255)
                  caccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTa                                                                                                                            >  7:149468/1‑149 (MQ=255)
                    ccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATTGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATc                                                                                                                          <  4:22226/149‑1 (MQ=255)
                    ccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATc                                                                                                                          <  3:81474/149‑1 (MQ=255)
                             ttAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCt                                                                                                                 >  8:88807/1‑149 (MQ=255)
                             ttAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTCCGACTGGAAATTACCGGGGAAGTCGCTCCGCAGACGGGCTGGATTATCGATTTCGCt                                                                                                                 >  2:65004/1‑149 (MQ=255)
                                    tttCACCTTAGAAGCCGCTCACAGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCAGCAAGGGCATTCATTTATGTTTCGACTGGAAATTACCGGTGAAGTCGATCGGCATACGGGCTGGATTATCGATTTCGCTCAACTa                                                                                                           >  8:251430/1‑148 (MQ=255)
                                       cACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTaaaa                                                                                                        <  2:5066/148‑1 (MQ=255)
                                          cTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAgcgg                                                                                                    >  4:24700/1‑149 (MQ=255)
                                           ttCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAgcggc                                                                                                   >  6:57267/1‑149 (MQ=255)
                                             cGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGACATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAgcggcg                                                                                                  <  4:203954/148‑1 (MQ=255)
                                                 gCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTaa                                                                                             <  3:24700/149‑1 (MQ=255)
                                                 gCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTaa                                                                                             >  2:190021/1‑149 (MQ=255)
                                                   cGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaac                                                                                           <  5:144228/149‑1 (MQ=255)
                                                      tCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTACAAGCGGCGTTTAaaccaa                                                                                        >  8:42211/1‑149 (MQ=255)
                                                       cACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaaccaac                                                                                       >  5:236198/1‑149 (MQ=255)
                                                        aCCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaaccaacc                                                                                      >  7:193147/1‑149 (MQ=255)
                                                        aCCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaaccaacc                                                                                      >  5:205507/1‑149 (MQ=255)
                                                        aCCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACAGGGGAAGTCGATACGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaaccaacc                                                                                      >  1:78471/1‑149 (MQ=255)
                                                          cGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTa                                                                                    >  7:60167/1‑149 (MQ=255)
                                                               aCCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAgc                                                                               >  1:70712/1‑149 (MQ=255)
                                                               aCCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAg                                                                                <  6:205507/148‑1 (MQ=255)
                                                                  acacGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGcc                                                                            >  1:210972/1‑149 (MQ=255)
                                                                    acGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTc                                                                          <  1:129669/149‑1 (MQ=255)
                                                                    acGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTc                                                                          <  6:236198/149‑1 (MQ=255)
                                                                          cGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCAc                                                                    <  8:149468/149‑1 (MQ=255)
                                                                              aGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCAtt                                                                <  1:190021/149‑1 (MQ=255)
                                                                                      aaTGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAAt                                                        <  8:193147/149‑1 (MQ=255)
                                                                                      aaTGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAAt                                                        <  6:47514/149‑1 (MQ=255)
                                                                                      aaTGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTAACGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAAt                                                        <  2:196692/149‑1 (MQ=255)
                                                                                               gCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTAAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTc                                                 >  3:125712/1‑147 (MQ=255)
                                                                                                           aTTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAAc                                   >  2:177146/1‑149 (MQ=255)
                                                                                                               cTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCaa                               >  7:245508/1‑149 (MQ=255)
                                                                                                               cTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCaa                               >  1:227621/1‑149 (MQ=255)
                                                                                                               cTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCaa                               >  2:226173/1‑149 (MQ=255)
                                                                                                                tttATGGTGCGACTGGAAATTACCGGGGAGGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAAc                              >  2:104931/1‑149 (MQ=255)
                                                                                                                tttATGGTGCGACTGGAAATTACCGGGGAGGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCATCGATCACCATTATCTCAATGATATTCCCTGTCTGGAAAACCAAAc                              >  2:108276/1‑149 (MQ=255)
                                                                                                                      ggtcGACTGGAAATTACCGGGGAAGGCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCg                         <  4:9702/145‑1 (MQ=255)
                                                                                                                      gTGCGACTGGAAATTACCGGGGAGGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGa                        <  1:104931/149‑1 (MQ=255)
                                                                                                                      gTGCGAATGTAAATTACCGGGGAGGTCGATCCGCATACGGGCTGGAGTAGCGATTTAGCTGAACTAAAAGCGGCGTTTAAACCACCCTCCGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGa                        <  1:108276/149‑1 (MQ=255)
                                                                                                                          gACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGtt                    <  1:75437/149‑1 (MQ=255)
                                                                                                                           aCTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAAt                                                        <  6:96859/112‑1 (MQ=255)
                                                                                                                           aCTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAAt                                                        >  5:96859/1‑112 (MQ=255)
                                                                                                                           aCTGGAAATTAACGGGGAAGTAGATCCGCATACGGGCTGGATTATCGATTTCTCTGAACTAAAAGCGGCGTTTAAACCAACCTACGATCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGTAAAACCCAACCAGCGAGGttt                   <  2:78471/149‑1 (MQ=255)
                                                                                                                               gAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTa                 <  2:227621/147‑1 (MQ=255)
                                                                                                                               gAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTAAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTa                 <  4:125712/147‑1 (MQ=255)
                                                                                                                               gAAATTACCGGAGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAg                <  3:151715/148‑1 (MQ=255)
                                                                                                                                 aaTTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCa              >  2:234756/1‑148 (MQ=255)
                                                                                                                                   ttACCGGTGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCaaaa           <  4:34563/149‑1 (MQ=255)
                                                                                                                                     aCCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAAt          <  1:174147/148‑1 (MQ=255)
                                                                                                                                        ggggAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGAt      <  7:88807/149‑1 (MQ=255)
                                                                                                                                           gAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTg   <  8:60167/149‑1 (MQ=255)
                                                                                                                                            aaGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTCAACTAAAAGCGGCGTTTTAACCAACCTACGAGCGCCTCGATCAGCATTATCTCAATGATATTCCATGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTgg  <  7:251430/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGG  >  NC_000913/2892204‑2892492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑