| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,862 | A→G | 6.6% | N184D (AAC→GAC) | ygfZ → | iron‑sulfur cluster repair protein, plumbagin resistance |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,862 | 0 | A | G | 6.6% | 181.9 / 2.8 | 60 | N184D (AAC→GAC) | ygfZ | iron‑sulfur cluster repair protein, plumbagin resistance |
| Reads supporting (aligned to +/- strand): ref base A (32/24); new base G (2/2); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAG > NC_000913/3041729‑3042009 | agaaaAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGt < 3:177070/149‑1 (MQ=255) gaaaAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCTGTGAAGCGGAACTGAACAATAGCCATCAGtg < 3:158572/149‑1 (MQ=255) gTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTTACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCAt > 6:44101/1‑148 (MQ=255) gTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTCACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCAt < 5:44101/148‑1 (MQ=255) gTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTCGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCAt > 2:203662/1‑148 (MQ=255) tCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTaaa > 1:52525/1‑148 (MQ=255) cAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACa > 1:103605/1‑149 (MQ=255) aagaagGCGCGCCCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTCATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACAtt > 5:112579/1‑149 (MQ=255) aagaagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACAtt < 2:52525/149‑1 (MQ=255) aagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGaa < 6:51745/149‑1 (MQ=255) aagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGATAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGaa < 8:234066/149‑1 (MQ=255) cgcgACCACTTGGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCgg < 7:198937/149‑1 (MQ=255) ccACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATAGAAGCGGGTTTc > 5:228528/1‑149 (MQ=255) cTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTaaa > 2:101844/1‑130 (MQ=255) cTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTaaa < 1:101844/130‑1 (MQ=255) gCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtga > 8:245197/1‑149 (MQ=255) cacCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCaa > 2:193818/1‑149 (MQ=255) cacCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCaa > 6:137308/1‑149 (MQ=255) aGAACGTTTCCTGATCGTAACCGATGAAGCTACAGCTAATATGCTGACCGATAAACTGCGCGGTCAAGCGGAACTGAACAATAGCCAACAGTGGCTGCCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCggg > 8:217825/1‑148 (MQ=255) aCGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGGACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtgattga < 4:223526/129‑1 (MQ=255) aCGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGGACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtgattga < 4:219959/129‑1 (MQ=255) aCGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGGACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtgattga > 3:223526/1‑129 (MQ=255) aCGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGGACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtgattga > 3:219959/1‑129 (MQ=255) gTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATgc < 2:526/130‑1 (MQ=255) gTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATgc > 1:526/1‑130 (MQ=255) cTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATccc > 4:90682/1‑149 (MQ=255) tGATCGTAACCGATGAAGATACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCca > 3:227680/1‑149 (MQ=255) gATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCca > 4:144294/1‑148 (MQ=255) aTCGTAACCGATGAAGCTACTGCTCATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCcac < 6:112579/148‑1 (MQ=255) aTCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCcac < 7:245197/148‑1 (MQ=255) tAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGc > 4:183544/1‑148 (MQ=255) tAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCg > 7:182231/1‑149 (MQ=255) aaCCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCgc > 2:65833/1‑148 (MQ=255) aaGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATcc > 6:200263/1‑132 (MQ=255) aaGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATcc < 5:200263/132‑1 (MQ=255) gCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAAtctc > 5:72130/1‑147 (MQ=255) gCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTcc < 2:103605/148‑1 (MQ=255) aaTATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTggg > 4:225314/1‑149 (MQ=255) gCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGTGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTa > 1:180107/1‑149 (MQ=255) cTGACCGATAAACTGCGCGGGGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTAt < 5:173914/149‑1 (MQ=255) tGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtgattg < 3:119913/87‑1 (MQ=255) tGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtgattg > 4:119913/1‑87 (MQ=255) aCCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAg > 8:30433/1‑149 (MQ=255) ccGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGcc < 3:190208/39‑1 (MQ=255) ccGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGcc > 4:190208/1‑39 (MQ=255) cGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATTCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCt > 2:95717/1‑149 (MQ=255) gATAAACTGCGCGGTGAAGCGGAACTGATCAATAGCCAAAAGTTCCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCACCAGCGGGCAGATTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGAATCAGCtt > 8:226856/1‑149 (MQ=255) gATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCtt > 3:96272/1‑149 (MQ=255) aTAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCttt < 3:225314/149‑1 (MQ=255) aaCTGCGCGGTGAAGCGGAACTGAACAATCGCCACCAGTGGCGGGCATTAAACATTGAAGCGGGTTTCCGGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTaa < 6:228528/148‑1 (MQ=255) aaCTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTaa < 1:203662/148‑1 (MQ=255) aCTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAg > 5:91139/1‑148 (MQ=255) gcgGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGc < 7:30433/149‑1 (MQ=255) cgGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCt < 3:144294/149‑1 (MQ=255) gTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 4:227680/149‑1 (MQ=255) tGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt > 5:175278/1‑148 (MQ=255) aGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTACCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTtata > 5:49268/1‑149 (MQ=255) cGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATAcc < 1:65833/149‑1 (MQ=255) aCTGAACAATAGCCAACAGTGGCTGGCTTTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGAc < 6:175278/149‑1 (MQ=255) tGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGAc > 3:8971/1‑147 (MQ=255) aaCAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATTCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAag < 1:95717/148‑1 (MQ=255) aaCAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTACCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAag < 6:49268/148‑1 (MQ=255) | AGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAG > NC_000913/3041729‑3042009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |