Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,148,217 C→T 7.5% Y107Y (TAC→TAT)  gpr → L‑glyceraldehyde 3‑phosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,148,2170CT 7.5% 160.3 / 2.6 54Y107Y (TAC→TAT) gprL‑glyceraldehyde 3‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (21/28);  new base T (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCG  >  NC_000913/3148074‑3148364
                                                                                                                                               |                                                                                                                                                   
tttGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGctc                                                                                                                                                >  7:112634/1‑149 (MQ=255)
tttGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGAGTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGctc                                                                                                                                                >  2:130746/1‑149 (MQ=255)
   gATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTATGGCTCTgg                                                                                                                                             >  8:9011/1‑149 (MQ=255)
   gATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTATGGCTCTgg                                                                                                                                             >  8:5415/1‑149 (MQ=255)
   gATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTg                                                                                                                                              <  3:223209/148‑1 (MQ=255)
    aTTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTATGGCTCTgg                                                                                                                                             <  7:5415/148‑1 (MQ=255)
    aTTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTATGGCTCTgg                                                                                                                                             <  7:9011/148‑1 (MQ=255)
     tgtAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATGTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCg                                                                                                                                           <  3:99963/147‑1 (MQ=255)
       tAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCgg                                                                                                                                          <  7:114453/148‑1 (MQ=255)
         gCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTc                                                                                                                                       <  3:157917/149‑1 (MQ=255)
           caacaaTTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCAc                                                                                                                                     >  8:183758/1‑149 (MQ=255)
            aacaaTTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACg                                                                                                                                    >  7:102993/1‑149 (MQ=255)
            aacaaTTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACg                                                                                                                                    >  2:103195/1‑149 (MQ=255)
                          gccTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCg                                                                                                                      >  6:219815/1‑149 (MQ=255)
                               cAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGc                                                                                                                 <  7:96363/149‑1 (MQ=255)
                               cAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGc                                                                                                                 >  5:40606/1‑149 (MQ=255)
                                    agtgcAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGa                                                                                                            <  1:186657/146‑1 (MQ=255)
                                     gcgcAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGAc                                                                                                           >  2:88134/1‑149 (MQ=255)
                                         agaagaGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCaaa                                                                                                       <  2:115968/149‑1 (MQ=255)
                                         agaagaGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCaaa                                                                                                       <  2:112563/149‑1 (MQ=255)
                                         agaagaGAACTTTGGGAGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCTGTTCACGTAAATACCTGCTCGCCAGCCTCGACCaaa                                                                                                       <  1:208606/149‑1 (MQ=255)
                                             gagaACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCt                                                                                                   >  4:62592/1‑149 (MQ=255)
                                                 aCTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGaa                                                                                                <  1:103195/148‑1 (MQ=255)
                                                  cTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGc                                                                                              <  8:102993/149‑1 (MQ=255)
                                                       gTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTAt                                                                                          <  2:31683/148‑1 (MQ=255)
                                                            ctgctgCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGt                                                                                            <  7:140833/141‑1 (MQ=255)
                                                            ctgctgAGGCAGGATTTTGCAGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACCATATCAGTCCCGGCCCTTAAGCCTCATGCGGTTCACGTAAATACCTTATCGACAGCCTCCACAAAAGACTTAAGCGTaagatcg                                                                                     >  8:140833/1‑142 (MQ=255)
                                                               ctgcGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCtt                                                                                   <  7:40921/147‑1 (MQ=255)
                                                                   gggAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTAt                                                                             <  2:136979/149‑1 (MQ=255)
                                                                          ttttGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGata                                                                      >  4:104910/1‑149 (MQ=255)
                                                                                 gCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTa                                                               <  3:104910/149‑1 (MQ=255)
                                                                                 gCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTa                                                               <  1:88134/149‑1 (MQ=255)
                                                                                     aTCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTa                                                               <  5:81192/145‑1 (MQ=255)
                                                                                     aTCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTa                                                               >  6:81192/1‑145 (MQ=255)
                                                                                     aTCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCCTATGGGGCTTGAGTATGTCGATATCTTTTActct                                                           >  8:241057/1‑149 (MQ=255)
                                                                                        gcgATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCAt                                                        <  8:229474/149‑1 (MQ=255)
                                                                                         cgATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATc                                                       <  8:112634/149‑1 (MQ=255)
                                                                                           aTGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATcgc                                                     >  3:187668/1‑149 (MQ=255)
                                                                                                   aTTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATg                                              >  7:198691/1‑148 (MQ=255)
                                                                                                       tctcTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGaaaa                                          <  4:187668/148‑1 (MQ=255)
                                                                                                       tctcTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGACGCGATGagag                                          <  3:86445/148‑4 (MQ=255)
                                                                                                         tctACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAACTACCTGCTCGCCAGCCTCGACCAACGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATAc                                       >  1:164251/1‑149 (MQ=255)
                                                                                                           tACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATAc                                       <  7:67572/147‑1 (MQ=255)
                                                                                                             ccAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGc                                     <  8:198691/147‑1 (MQ=255)
                                                                                                                   ctggctACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGgaa                             <  1:130746/149‑1 (MQ=255)
                                                                                                                   ctggctACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGgaa                             <  3:62592/149‑1 (MQ=255)
                                                                                                                      gctACGATATTTGGCCCGTCCATTACGGCTCTGGCGGTTCACGTAAATACCTGATCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAATTACGCCGATGgaaga                           >  6:17026/1‑148 (MQ=255)
                                                                                                                      gctACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGgaagaa                          <  7:183758/149‑1 (MQ=255)
                                                                                                                           gATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGa                                  >  5:113768/1‑136 (MQ=255)
                                                                                                                           gATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGa                                  <  6:113768/136‑1 (MQ=255)
                                                                                                                                   gcccggcccTTACGGCTCTGGCGGGTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTgcgc             >  3:17775/1‑149 (MQ=255)
                                                                                                                                     ccggcccTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTg           >  3:62008/1‑149 (MQ=255)
                                                                                                                                      cggcccTTACGGCTCTGGCGGGTCACGTAAATACCTGCTCGCAAGCCTCGAACAAATCCTGACTCGTATGGGGCGTGATTAGGTAGATATCTTTTACTCTCAGAGCGTCGATGAAAATACGCCGATGGAAGAAACCTCCTCGGCTCTgt          >  6:99/1‑148 (MQ=255)
                                                                                                                                        gcccTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCATGAAGCGTATGGGGCATGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGAAGATGGAAGAAACCGCCACTGCGCTGGCt        >  8:30123/1‑149 (MQ=255)
                                                                                                                                               cGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCg  <  6:40606/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCG  >  NC_000913/3148074‑3148364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑