Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,161,653 G→A 8.5% R339R (CGC→CGT)  ftsP ← septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,161,6530GA 8.5% 140.0 / 2.5 47R339R (CGC→CGT) ftsPseptal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base A (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTT  >  NC_000913/3161506‑3161801
                                                                                                                                                   |                                                                                                                                                    
tACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgc                                                                                                                                                     <  7:32046/149‑1 (MQ=255)
   cGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCa                                                                                                                                                  >  1:22400/1‑149 (MQ=255)
       cAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCgg                                                                                                                                              <  2:92472/149‑1 (MQ=255)
         gCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGaa                                                                                                                                            <  1:222207/149‑1 (MQ=255)
           gTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGa                                                                                                                                          <  2:147236/149‑1 (MQ=255)
                 aCGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGAAAGActgtct                                                                                                                                    >  6:148708/1‑149 (MQ=255)
                    ttCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGAAAGACTGTCtgtg                                                                                                                                 <  5:148708/149‑1 (MQ=255)
                                       aTCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCTTCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCgg                                                                                                              >  3:94026/1‑149 (MQ=255)
                                        tCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGt                                                                                                             <  3:74080/149‑1 (MQ=255)
                                           aTACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTAATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTgg                                                                                                          >  4:63690/1‑149 (MQ=255)
                                            tACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGc                                                                                                                 <  5:110441/141‑1 (MQ=255)
                                            tACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGc                                                                                                                 >  6:110441/1‑141 (MQ=255)
                                               ggTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGgcg                                                                                                       >  4:218870/1‑148 (MQ=255)
                                                gTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGgcg                                                                                                       >  6:29302/1‑147 (MQ=255)
                                                    aCGTCCCACAGCTGTCCATTACTACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGt                                                                                                 <  8:197772/149‑1 (MQ=255)
                                                    aCGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGt                                                                                                 <  5:144082/149‑1 (MQ=255)
                                                     cGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGtt                                                                                                <  1:174773/149‑1 (MQ=255)
                                                        cccACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGACAGCAAGCGCATCGGAAGACTGTCTGTGCCCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAg                                                                                              >  5:208574/1‑148 (MQ=255)
                                                            caGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCa                                                                                         <  5:29302/149‑1 (MQ=255)
                                                               cTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAggg                                                                                      <  1:6326/149‑1 (MQ=255)
                                                                    cATTAATACCCGGGTCATCACCCAGACTGATATAGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGa                                                                                  >  8:136180/1‑148 (MQ=255)
                                                                            cccGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGaa                                                                          >  7:59622/1‑148 (MQ=255)
                                                                             ccGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCa                                                                             >  8:69545/1‑144 (MQ=255)
                                                                             ccGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCa                                                                             <  7:69545/144‑1 (MQ=255)
                                                                              cGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAACGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATAc                                                                       >  3:126788/1‑149 (MQ=255)
                                                                              cGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAACGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATAc                                                                       >  3:129998/1‑149 (MQ=255)
                                                                               gggTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCTTCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACt                                                                      <  4:94026/149‑1 (MQ=255)
                                                                                     tcaCCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATgg                                                                <  2:22400/149‑1 (MQ=255)
                                                                                       aCCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCt                                                              >  6:230975/1‑149 (MQ=255)
                                                                                       aCCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCt                                                              >  7:188885/1‑149 (MQ=255)
                                                                                        cccAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCt                                                              >  1:34513/1‑148 (MQ=255)
                                                                                        cccAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGACAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTc                                                             <  6:208574/149‑1 (MQ=255)
                                                                                            gACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTg                                                                     >  5:38649/1‑137 (MQ=255)
                                                                                            gACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTg                                                                     <  6:38649/137‑1 (MQ=255)
                                                                                                 atatCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAggg                                                                                      <  5:222276/115‑1 (MQ=255)
                                                                                                 atatCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAggg                                                                                      >  6:222276/1‑115 (MQ=255)
                                                                                                    tCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCAc                                                  >  2:21371/1‑148 (MQ=255)
                                                                                                        gACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAACGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATa                                             <  4:129998/149‑1 (MQ=255)
                                                                                                        gACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAACGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATa                                             <  4:126788/149‑1 (MQ=255)
                                                                                                           tGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGa                                          >  7:20092/1‑149 (MQ=255)
                                                                                                                        aCCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTcg                             <  3:218870/149‑1 (MQ=255)
                                                                                                                         gcAGCAATGATTTCACTTGGCAGGAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAACGCCGGTTGGGCGCAGCGTTAGCACCAGGGTTGAAACCAGAATTCTGGATGGCTCAAAGAAGCCACCAATTCGATCAACAATGCTcgc                            <  4:50441/148‑1 (MQ=255)
                                                                                                                               aTGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTg                                                                     <  4:59370/102‑1 (MQ=255)
                                                                                                                               aTGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTg                                                                     >  3:59370/1‑102 (MQ=255)
                                                                                                                                       aGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAgg              <  8:59622/149‑1 (MQ=255)
                                                                                                                                         ttGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGt             >  7:74382/1‑148 (MQ=255)
                                                                                                                                            gcagcaAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGAt          <  5:230975/148‑1 (MQ=255)
                                                                                                                                            gcagcaAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATc         <  3:63690/149‑1 (MQ=255)
                                                                                                                                                   gcgcATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACtt  <  1:21371/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCACCAGGGTAGAAACCAGAATACTGGATGGCTCAAAGAAGCCACGAATACGATCAACAATGCTCGCCGCTTCGCCACAGGTGATCGACACTT  >  NC_000913/3161506‑3161801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑