Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 395,468 A→T 7.0% Y274N (TAT→AAT)  ampH ← D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913395,4680AT 7.0% 164.8 / 3.9 57Y274N (TAT→AAT) ampHD‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base T (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

CGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCC  >  NC_000913/395326‑395610
                                                                                                                                              |                                                                                                                                              
cgacCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATc                                                                                                                                          <  3:117582/149‑1 (MQ=255)
   cccTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGa                                                                                                                                       <  7:247100/149‑1 (MQ=255)
    ccTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGAt                                                                                                                                      <  7:2803/149‑1 (MQ=255)
           cGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACaaa                                                                                                                                <  7:209978/148‑1 (MQ=255)
                   tatataCCCAACCTAACCCAAGCGCATCCGCTTTGCCGGGCACATCCAAGCCAATCACTTTCCTAAACTGCCCCCGCTGATAAAACAACGTCTCCATCCGATCTGGCTGGTTACTGCGCTGATAAAAATCAGATGACAAATActgcag                                                                                                                        >  3:199246/1‑146 (MQ=255)
                        aCCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATc                                                                                                                    >  3:28548/1‑147 (MQ=255)
                          ccAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAgc                                                                                                                >  2:239202/1‑149 (MQ=255)
                               cTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGTCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGcat                                                                                                            >  5:219877/1‑148 (MQ=255)
                               cTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGcatc                                                                                                           <  3:61393/149‑1 (MQ=255)
                                      aaGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGc                                                                                                    <  8:54352/149‑1 (MQ=255)
                                            aTCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCg                                                                                              >  8:105633/1‑149 (MQ=255)
                                                gCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATACAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTgg                                                                                           >  8:175939/1‑148 (MQ=255)
                                                    tGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATa                                                                                       <  7:8577/148‑1 (MQ=255)
                                                     gCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTgg                                                                                           >  5:237260/1‑143 (MQ=255)
                                                     gCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTgg                                                                                           <  6:237260/143‑1 (MQ=255)
                                                     gCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTg                                                                                            <  3:85145/142‑1 (MQ=255)
                                                     gCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTg                                                                                            >  4:85145/1‑142 (MQ=255)
                                                      ccGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGTCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGa                                                                                     <  6:219877/148‑1 (MQ=255)
                                                           gACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGAcgccgc                                                                               >  3:83629/1‑149 (MQ=255)
                                                                 cATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGc                                                                         >  7:198730/1‑149 (MQ=255)
                                                                   tGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACTTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGcc                                                                        >  5:15193/1‑148 (MQ=255)
                                                                      cAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAgc                                                                                                                >  8:180337/1‑105 (MQ=255)
                                                                      cAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAgc                                                                                                                <  7:180337/105‑1 (MQ=255)
                                                                      cAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCAGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCAAATCGCCAGGCGAGGAATACACGCCGCCGCTGCCAAt                                                                     >  2:187365/1‑148 (MQ=255)
                                                                       aaTCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTg                                                                   <  1:239202/149‑1 (MQ=255)
                                                                        aTCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTgc                                                                  >  8:22099/1‑149 (MQ=255)
                                                                           aCTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGCCGCCGCCGCTGCCAATTgccgc                                                               >  2:231468/1‑149 (MQ=255)
                                                                            cTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTgccgc                                                               >  3:158410/1‑148 (MQ=255)
                                                                             tttCGTAAACTGCGCGTGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATTAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTgccgc                                                               >  8:137330/1‑147 (MQ=255)
                                                                             tttCGTAAACTGCGCGTGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATTAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTgccgc                                                               >  8:140826/1‑147 (MQ=255)
                                                                              ttCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAg                                                            >  4:36672/1‑149 (MQ=255)
                                                                                  tAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGAcgc                                                                                  <  7:124262/123‑1 (MQ=255)
                                                                                  tAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGAcgc                                                                                  >  8:124262/1‑123 (MQ=255)
                                                                                    aaCTGCGCGCGCTTTTAAATCAACGTCTGCAAGCGATCTGCCTGGTTACTGCTCTGATAATAATCAGATGAAAAATACTGCTGCATCCAGCTCATCATATCGCCAGGCGTTGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGtt                                                      <  7:205929/149‑1 (MQ=255)
                                                                                    aaCTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCgtgt                                                       <  7:109513/148‑1 (MQ=255)
                                                                                     aCTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTa                                                     >  8:102292/1‑149 (MQ=255)
                                                                                          gcgcgcTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCa                                                <  6:83745/149‑1 (MQ=255)
                                                                                            gcgcTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCgtgt                                                       >  4:34966/1‑140 (MQ=255)
                                                                                            gcgcTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCgtgt                                                       <  3:34966/140‑1 (MQ=255)
                                                                                             ctcTGATAAAGCAACGTCTTCATGCGAGCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCTCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACgg                                             <  6:175835/147‑1 (MQ=255)
                                                                                                 gATAAATCAACGTCTGCATGCGATCTGACAGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCTCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTg                                         >  1:121938/1‑149 (MQ=255)
                                                                                                     tatcatcGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCGAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCTCCATCGTGTTATTGCACGGACTCGCa                                      <  4:199246/147‑1 (MQ=255)
                                                                                                      aTCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCAcc                                    >  3:63707/1‑149 (MQ=255)
                                                                                                             tCTGCATGCGATCTTCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCa                             <  4:28548/149‑1 (MQ=255)
                                                                                                              cTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCACTCa                             <  6:15193/148‑1 (MQ=255)
                                                                                                                 cATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGc                           <  5:209678/147‑1 (MQ=255)
                                                                                                                   tGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAAc                        >  5:117961/1‑148 (MQ=255)
                                                                                                                     cGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCa                      <  7:22099/148‑1 (MQ=255)
                                                                                                                     cGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCa                      <  4:63707/148‑1 (MQ=255)
                                                                                                                          tGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAAc                >  2:119065/1‑149 (MQ=255)
                                                                                                                                  tACTGCGCTGATTAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCTCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCAc        <  7:140826/149‑1 (MQ=255)
                                                                                                                                   aCTGCGCTGATTAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCAc        <  7:137330/148‑1 (MQ=255)
                                                                                                                                   aCTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCAc        <  4:83629/148‑1 (MQ=255)
                                                                                                                                   aCTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCAAGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCAc        <  4:158410/148‑1 (MQ=255)
                                                                                                                                       cgcTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGa     <  7:105633/147‑1 (MQ=255)
                                                                                                                                       cgcTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATc   >  5:149555/1‑149 (MQ=255)
                                                                                                                                        gcTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATcc  <  8:198730/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAACTGCGCGCGCTGATAAATCAACGTCTGCATGCGATCTGCCTGGTTACTGCGCTGATAAAAATCAGATGACAAATACTGCTGCATCCAGCGCATCATATCGCCAGGCGTGGAATAGACGCCGCCGCTGCCAATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCC  >  NC_000913/395326‑395610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑