Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,345,295 A→G 6.2% K193K (AAA→AAG rpoN → RNA polymerase, sigma 54 (sigma N) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,345,2950AG 6.2% 197.9 / 2.6 66K193K (AAA→AAGrpoNRNA polymerase, sigma 54 (sigma N) factor
Reads supporting (aligned to +/- strand):  ref base A (34/27);  new base G (2/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGTCTG  >  NC_000913/3345147‑3345439
                                                                                                                                                    |                                                                                                                                                
tcgtcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCaaaa                                                                                                                                                  <  5:66648/149‑1 (MQ=255)
  gtcgATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATCAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGCCGGCAAAAg                                                                                                                                                 >  6:63966/1‑148 (MQ=255)
        gCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAGGATCTGCg                                                                                                                                          >  4:45887/1‑149 (MQ=255)
              gatgaAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTg                                                                                                                                    >  2:131610/1‑149 (MQ=255)
                         ttATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAGGATCTGCGTGACTGTCTGCTGATcc                                                                                                                         <  3:45887/149‑1 (MQ=255)
                         ttATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATc                                                                                                                          >  2:220686/1‑148 (MQ=255)
                               gACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAActct                                                                                                                   >  7:81730/1‑149 (MQ=255)
                                 cTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTcc                                                                                                                 <  8:107075/149‑1 (MQ=255)
                                 cTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTcc                                                                                                                 <  8:81730/149‑1 (MQ=255)
                                    tCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATc                                                                                                                          >  7:170674/1‑137 (MQ=255)
                                    tCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATc                                                                                                                          <  8:170674/137‑1 (MQ=255)
                                    tCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTccc                                                                                                                <  4:123526/147‑1 (MQ=255)
                                      ccGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAAtt                                                                                                            >  6:87005/1‑149 (MQ=255)
                                       cGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTc                                                                                                           <  1:131610/149‑1 (MQ=255)
                                            gAAGATATTCTCGAAAGTATAGGCGATGACGAGATCGACATCGACGAAGTTGAAGCCGGCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGAt                                                                                                        <  5:227913/147‑1 (MQ=255)
                                            gAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATaa                                                                                                      <  7:98096/149‑1 (MQ=255)
                                             aaGATATTCTCGAAAGTATAGGCGATTAAGAGATCGACATCGCCGAATTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCGGCTCCAAATCTCCCAATTCGAt                                                                                                        >  5:139527/1‑146 (MQ=255)
                                              aGATATTCTCGAAAGTATAGGCGATGATGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGa                                                                                                    <  4:117450/149‑1 (MQ=255)
                                                  atTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCAc                                                                                                >  7:242899/1‑149 (MQ=255)
                                                         aaaGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTgg                                                                                         <  1:129077/149‑1 (MQ=255)
                                                             tataGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCTTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTg                                                                                      >  3:136475/1‑148 (MQ=255)
                                                                    gATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGc                                                                              >  3:91081/1‑149 (MQ=255)
                                                                      tGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGGGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCa                                                                            <  4:145735/149‑1 (MQ=255)
                                                                          gagaTAGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAGGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACt                                                                        >  7:91427/1‑149 (MQ=255)
                                                                             atcgacatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGc                                                                              >  6:136865/1‑140 (MQ=255)
                                                                             atcgacatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGc                                                                              <  5:136865/140‑1 (MQ=255)
                                                                              tcgacatcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATc                                                                    <  7:52640/149‑1 (MQ=255)
                                                                                   atcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTac                                                               >  1:57287/1‑148 (MQ=255)
                                                                                    tcgacGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGc                                                              >  6:103993/1‑149 (MQ=255)
                                                                                      gacgaAGTTGAAGCCGTCCTTAAGCGGGTCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGa                                                            >  6:158588/1‑149 (MQ=255)
                                                                                      gacgaAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGa                                                            >  5:124721/1‑149 (MQ=255)
                                                                                      gacgaAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGGCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGa                                                            <  5:158588/149‑1 (MQ=255)
                                                                                         gacgTTGAAGCCGTCCTTATGCGGATCGAACGGTTTGATCCGGTCGGTGTGGCGGCATATGATCTGCGTGTCTGTCTGCTGATCCAACTCTCGCAATTCGATAAGACCACGCGGTGGCTGGATGAGGCCAGACAGATCATTAGCGatca                                                         <  6:165417/146‑1 (MQ=255)
                                                                                            gTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATGTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCAtct                                                      <  8:118735/149‑1 (MQ=255)
                                                                                            gTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCAtct                                                      >  4:164599/1‑149 (MQ=255)
                                                                                                aaGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGa                                                   <  7:228089/148‑1 (MQ=255)
                                                                                                     gTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGtt                                             <  1:10459/149‑1 (MQ=255)
                                                                                                     gTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGt                                              >  5:211070/1‑148 (MQ=255)
                                                                                                        cTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGatcatc                                                       >  4:191015/1‑136 (MQ=255)
                                                                                                        cTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGGCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGatcatc                                                       <  3:191015/136‑1 (MQ=255)
                                                                                                          tAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGcc                                         >  4:199730/1‑148 (MQ=255)
                                                                                                            aGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCaa                                       <  1:220686/148‑1 (MQ=255)
                                                                                                             gCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAAt                                      >  1:124097/1‑148 (MQ=255)
                                                                                                             gCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATc                                     <  4:126469/149‑1 (MQ=255)
                                                                                                             gCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACACTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTATCGATCATCTCGATCTGTTAGCCAAt                                      >  2:165280/1‑148 (MQ=255)
                                                                                                                 aTCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGCTCATTAGCGCTCATCTCGATCTGTTAGCCAATCACGa                                 >  2:127736/1‑149 (MQ=255)
                                                                                                                     aaCGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGCGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTc                             >  4:44680/1‑149 (MQ=255)
                                                                                                                     aaCGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATATGCGTGACTGTCTGCTGATCCATCTCTCCCAATTCGATAAGACCACGCCGTTGCTGGAAGAGTCCAGACTGAACATTAGCGAACATCTCGATCTGTTAGCCAATCACGACTTc                             >  1:80917/1‑149 (MQ=255)
                                                                                                                      aCGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTcc                            >  8:241867/1‑149 (MQ=255)
                                                                                                                         gTTTGATCCGGTCGGTGTGGCGGCAAAGGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCa                         <  8:91427/149‑1 (MQ=255)
                                                                                                                         gTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCa                         >  6:191334/1‑149 (MQ=255)
                                                                                                                          tttGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCAc                        >  4:73866/1‑149 (MQ=255)
                                                                                                                            tGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACt                       <  3:164599/148‑1 (MQ=255)
                                                                                                                              aTCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACttt                     >  1:113491/1‑148 (MQ=255)
                                                                                                                              aTCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACttt                     >  6:107638/1‑148 (MQ=255)
                                                                                                                               tCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTaa                   <  7:241867/149‑1 (MQ=255)
                                                                                                                                 cggtcggtGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAAt                  <  6:139527/148‑1 (MQ=255)
                                                                                                                                  ggtcggtGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATgc                <  2:124097/149‑1 (MQ=255)
                                                                                                                                   gtcggtGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCa                                        >  2:47650/1‑124 (MQ=255)
                                                                                                                                   gtcggtGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCa                                        <  1:47650/124‑1 (MQ=255)
                                                                                                                                    tcggtGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATgcgc              <  5:63966/149‑1 (MQ=255)
                                                                                                                                    tcggtGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATgcgc              <  8:242899/149‑1 (MQ=255)
                                                                                                                                       gtgtGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTc           <  7:161835/149‑1 (MQ=255)
                                                                                                                                          tgGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCAcg        >  4:157173/1‑149 (MQ=255)
                                                                                                                                              ggcAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGTc    >  8:214/1‑149 (MQ=255)
                                                                                                                                               gcAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGTCt   >  7:246419/1‑149 (MQ=255)
                                                                                                                                                cAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGTCt   >  2:137550/1‑148 (MQ=255)
                                                                                                                                                 aaaaGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGTCTg  >  7:228461/1‑148 (MQ=255)
                                                                                                                                                 aaaaGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGTCTg  >  3:169228/1‑148 (MQ=255)
                                                                                                                                                 aaaaGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGTCTg  >  8:72353/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCCGGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGGAAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGTCTG  >  NC_000913/3345147‑3345439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: