Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,390,718 C→A 7.0% Q437H (CAG→CAT)  tldD ← putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,390,7180CA 7.0% 184.0 / 2.7 57Q437H (CAG→CAT) tldDputative peptidase
Reads supporting (aligned to +/- strand):  ref base C (26/27);  new base A (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

AAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTT  >  NC_000913/3390577‑3390861
                                                                                                                                             |                                                                                                                                               
aaaTTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCGAACCATCGAAATCTGCTGCa                                                                                                                                          >  2:222812/1‑149 (MQ=255)
 aaTTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCAt                                                                                                                                         <  2:211320/149‑1 (MQ=255)
   ttattaCGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATgg                                                                                                                                       <  8:176941/149‑1 (MQ=255)
      ttaCGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGttt                                                                                                                                    <  2:58687/149‑1 (MQ=255)
      ttaCGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGttt                                                                                                                                    <  4:74318/149‑1 (MQ=255)
         cGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCg                                                                                                                                  <  7:171020/148‑1 (MQ=255)
         cGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGGTGCCAACCATCGAAATCTGCTGCATGGTTTCGa                                                                                                                                 >  7:95884/1‑149 (MQ=255)
          gctttACGGCCAACAGTCTGGTTATCGACTTTCAACGTTGGCTGGCCCACGGCAACCGGCAAACTTTGGCCTTCTTTACCGCAGACACCCACGGCGTTATCCTGTTTCAGGTCGTTGCGAACCATCGAAATCTGCTGCATGGTTTCGa                                                                                                                                 <  1:83778/144‑1 (MQ=255)
            actcccGCCACCACTCAGGTTATCGACCTTCAACGTCGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGCTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATAc                                                                                                                              <  6:192257/145‑1 (MQ=255)
             gTACCGCCAACAGTCAGGTTATCGTCTTTCAACGTTGGGTGGCCCTCGCCAACCGGCAATCTTTGCCCTTCTTTAGCGCAGACACCCACGCCGTTATCCAGTTTGAGGTCGTTGCCAACCTTCGAAATCTGCTGCATGGTTTCGATAcc                                                                                                                             <  5:158501/149‑1 (MQ=255)
                 cGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGACAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGaa                                                                                                                         <  1:74853/149‑1 (MQ=255)
                  gCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAAc                                                                                                                        <  1:247099/149‑1 (MQ=255)
                   ccAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAAc                                                                                                                        >  3:44169/1‑148 (MQ=255)
                             ggTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGtt                                                                                                             <  8:95884/149‑1 (MQ=255)
                              gTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTg                                                                                                            >  6:4457/1‑149 (MQ=255)
                                  tCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTgcgc                                                                                                         <  3:54414/148‑1 (MQ=255)
                                  tCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGGCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTgcgc                                                                                                         <  1:215660/148‑1 (MQ=255)
                                         tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCAcc                                                                                                 >  5:178588/1‑149 (MQ=255)
                                         tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATATGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCttt                                                                                                     >  4:231555/1‑145 (MQ=255)
                                         tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATATGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCttt                                                                                                     <  3:231555/145‑1 (MQ=255)
                                         tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATATGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCttt                                                                                                     <  7:100334/145‑1 (MQ=255)
                                         tCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATATGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCttt                                                                                                     >  8:100334/1‑145 (MQ=255)
                                          cAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCg                                                                                                >  2:134803/1‑149 (MQ=255)
                                            aCGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTGCCGGAGCCACCCACGACGTTATCAAGTTTCAGGTCGTTGCCAACCATAGAACTCGGCTGCATGGTGTCGATACCGGAACCAATCAACGTTGCGCCTTTCCCAGGc                                                                                              >  2:228159/1‑149 (MQ=255)
                                            aCGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGc                                                                                              >  2:20792/1‑149 (MQ=255)
                                                        ccACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACAGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTa                                                                                  >  2:61652/1‑149 (MQ=255)
                                                          aCGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTAcc                                                                                >  3:63079/1‑149 (MQ=255)
                                                               aaCCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGtttt                                                                           <  1:61652/149‑1 (MQ=255)
                                                                   ggCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCaa                                                                        >  6:73323/1‑148 (MQ=255)
                                                                    gCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATc                                                                      <  4:92451/149‑1 (MQ=255)
                                                                     cAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAAt                                                                       >  1:143640/1‑147 (MQ=255)
                                                                       aaCTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGa                                                                   <  1:33440/149‑1 (MQ=255)
                                                                             gCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGc                                                              <  5:4457/148‑1 (MQ=255)
                                                                              cccTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCtt                                                            <  6:102276/149‑1 (MQ=255)
                                                                               ccTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCGGATATGCTTc                                                           >  3:151180/1‑149 (MQ=255)
                                                                                  tcttTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTg                                                         >  2:213781/1‑148 (MQ=255)
                                                                                    tttACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAg                                                      >  4:195622/1‑149 (MQ=255)
                                                                                        ccGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAAc                                                                                                                        <  8:230202/79‑1 (MQ=255)
                                                                                        ccGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAAc                                                                                                                        >  7:230202/1‑79 (MQ=255)
                                                                                              acacCCACGCCGTTATCCAGTTTCAGGTCGTTGCGAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAAc                                            <  1:222812/149‑1 (MQ=255)
                                                                                                  ccACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAAt                                                                                                                    <  4:174467/73‑1 (MQ=255)
                                                                                                  ccACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAAt                                                                                                                    >  3:174467/1‑73 (MQ=255)
                                                                                                     cGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTg                                     <  1:134803/149‑1 (MQ=255)
                                                                                                          ttATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCGTTCACCGGCTTCGTTACTTTACCCTTTTCAATCAGATATGCTTCGGAAGTGGAGAAAACGAATTTGCCg                                  >  5:235854/1‑147 (MQ=255)
                                                                                                              ccAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGt                             >  3:113843/1‑148 (MQ=255)
                                                                                                                 gTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGata                         <  1:20792/149‑1 (MQ=255)
                                                                                                                        gTCGTTGCCACCCATCGAAATCTGCTGCATGGGTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCt                  >  2:164720/1‑149 (MQ=255)
                                                                                                                        gTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCt                  >  1:163202/1‑149 (MQ=255)
                                                                                                                               ccAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGcc           <  6:178588/149‑1 (MQ=255)
                                                                                                                               ccAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGc            >  8:19129/1‑148 (MQ=255)
                                                                                                                                cAACCATCGAAATCTGCGGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCCCCGGCTTCGTTACTTTACCGTTTTCAATCAGATAAGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGcca          <  4:44169/149‑1 (MQ=255)
                                                                                                                                   gcttCGAAATCTGCTTCATTGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCTGCTTCGTTACTTTACCTTTTTCAATCTGATATGCTTCTTAAGTGGAGAAAACGAATTTGCCGGAGGt                             <  6:206623/124‑1 (MQ=255)
                                                                                                                                   ccATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTATCACCGGCTTCGTTACTTTACCGTTTTAAATCACATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGt                             >  5:206623/1‑127 (MQ=255)
                                                                                                                                   ccATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTATCACCGGCTTCGTTACATTACCGTTTTCAATCAGATATGATTCTGAAGTGGATAAAACGAATTTGCCGGAGGTGATATCCTCCTGACCGccacc        >  5:79220/1‑148 (MQ=255)
                                                                                                                                     aTCGAAATCTGCTGCATGGTTTCGATAACGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCaat     <  2:38216/149‑2 (MQ=255)
                                                                                                                                       cGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGt   >  7:250783/1‑149 (MQ=255)
                                                                                                                                        gAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGtt  >  5:23663/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                               
AAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTACCGCAGACACCCACGCCGTTATCCAGTTTCAGGTCGTTGCCAACCATCGAAATCTGCTGCATGGTTTCGATACCGGAACCAATCAACGTTGCGCCTTTCACCGGCTTCGTTACTTTACCGTTTTCAATCAGATATGCTTCTGAAGTGGAGAAAACGAATTTGCCGGAGGTGATATCCACCTGACCGCCACCAAAGTT  >  NC_000913/3390577‑3390861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑