Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 435,172 T→C 8.3% C12C (TGT→TGC)  nusB → transcription antitermination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913435,1720TC 8.3% 135.6 / 3.6 50C12C (TGT→TGC) nusBtranscription antitermination protein
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base C (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

GAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCA  >  NC_000913/435025‑435316
                                                                                                                                                   |                                                                                                                                                
gAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAgtgt                                                                                                                                                  <  5:167904/148‑1 (MQ=255)
          gCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAgg                                                                                                                                        <  8:217990/148‑1 (MQ=255)
            aCCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGCGCCGTCCAGgcgc                                                                                                                                     >  8:88244/1‑149 (MQ=255)
                         acaaAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTgg                                                                                                                          >  5:58829/1‑147 (MQ=255)
                                tGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTcc                                                                                                                 >  1:59977/1‑149 (MQ=255)
                                  cAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTccc                                                                                                                <  7:2040/148‑1 (MQ=255)
                                    gAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTc                                                                                                                                            <  8:90331/118‑1 (MQ=255)
                                    gAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTc                                                                                                                                            >  7:90331/1‑118 (MQ=255)
                                     aaGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGCGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGaa                                                                                                            <  7:88244/149‑1 (MQ=255)
                                         tGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGa                                                                                                         >  3:133724/1‑148 (MQ=255)
                                            aCTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACa                                                                                                       >  2:18428/1‑147 (MQ=255)
                                            aCTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATc                                                                                                     >  2:62376/1‑149 (MQ=255)
                                                aCCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCt                                                                                                  <  8:218932/148‑1 (MQ=255)
                                                 ccGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGa                                                                                                <  5:215640/149‑1 (MQ=255)
                                                    cgcTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCTCTGATGt                                                                                             <  6:229961/149‑1 (MQ=255)
                                                       ttGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTg                                                                                           >  4:41773/1‑148 (MQ=255)
                                                       ttGAAATGATTAATGTATTGAAAGCCATCAAGGCCAGAAATGAGTAAGGGGAAATCCGTGAAACCTTCTGCTCGTCGCCGCGCACGTGAGTGAGCCGTCAAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTg                                                                                           >  3:161322/1‑148 (MQ=255)
                                                         gAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGCGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGtt                                                                                            >  8:138738/1‑145 (MQ=255)
                                                         gAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGCGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGtt                                                                                            <  7:138738/145‑1 (MQ=255)
                                                          aaaTGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATa                                                                                       >  3:70535/1‑149 (MQ=255)
                                                           aaTGATTACTGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAACCCTGCTGCTCGGCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATAc                                                                                      <  2:178133/149‑1 (MQ=255)
                                                           aaTGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATAc                                                                                      <  3:41773/149‑1 (MQ=255)
                                                              gATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAg                                                                                   >  3:218695/1‑149 (MQ=255)
                                                                   aTGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTcc                                                                               <  1:62376/148‑1 (MQ=255)
                                                                   aTGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCt                                                                              <  8:241855/149‑1 (MQ=255)
                                                                    tGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTg                                                                                                                           <  3:67630/103‑1 (MQ=255)
                                                                    tGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTg                                                                             >  1:49482/1‑149 (MQ=255)
                                                                    tGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTACTg                                                                             >  6:75825/1‑149 (MQ=255)
                                                                    tGTATTGAAAGCCAACAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTg                                                                                                                           >  4:67630/1‑103 (MQ=255)
                                                                             aGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGa                                                                                                <  2:233620/121‑1 (MQ=255)
                                                                             aGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGa                                                                                                >  1:233620/1‑121 (MQ=255)
                                                                                cATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGct                                                                                                                                    >  1:188443/1‑82 (MQ=255)
                                                                                cATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGct                                                                                                                                    <  2:188443/82‑1 (MQ=255)
                                                                                      ggCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTaaaa                                                           <  6:58829/149‑1 (MQ=255)
                                                                                       gCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCTCTCGTGATTGTGCCGACCAGGCTCTCTACTCCTGGCAGTTGTCACAGAACGACAACGCAGAACTTGAATACCAGTTCGTCGCTGAACAGGATCTaaaa                                                           >  8:67809/1‑148 (MQ=255)
                                                                                                   tAAGGGTAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATa                                                                                       <  2:238788/108‑1 (MQ=255)
                                                                                                        ggAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTAc                                         >  1:200040/1‑149 (MQ=255)
                                                                                                             tCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCg                                    >  4:217402/1‑149 (MQ=255)
                                                                                                                     cccTGCTGCTCGTCGCTGCGCTCGGGAGTGTGCCGTCCAGGCGCGCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAgctgc                            <  2:225544/148‑1 (MQ=255)
                                                                                                                     aCCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGATGACGTCCTGTACTTCCGTGAgctgc                            >  4:130535/1‑149 (MQ=255)
                                                                                                                        tgctgcTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTgg                         >  2:173515/1‑149 (MQ=255)
                                                                                                                             ctCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCggg                    >  1:68467/1‑149 (MQ=255)
                                                                                                                             ctCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCggg                    <  1:173515/149‑1 (MQ=255)
                                                                                                                                     cgcgCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGAc            <  2:200040/149‑1 (MQ=255)
                                                                                                                                         cTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGGTGGACCAGAACGTCATCGCTGATGTTGAATAACAGTTCCTGGCTTAACAGGATGTAAAAGACTTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAAt        <  5:75825/149‑1 (MQ=255)
                                                                                                                                            gTGAGTGTGCCGTCCAGGCGCTCTACTCCTTGCTGTTGTCCCAGAACGACATCGCTTATGTTGAATACCTGTTCCTGGCTTAACAGTATGTAAAAGACGTTGACGTCCTGTACTTCCGTGTGCTGCTGGCCGGGGTGGCGACTAATAcc     <  4:161322/149‑1 (MQ=255)
                                                                                                                                            gTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCt                                                                          <  7:217039/80‑1 (MQ=255)
                                                                                                                                            gTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCt                                                                          >  8:217039/1‑80 (MQ=255)
                                                                                                                                            gTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATAcc     >  4:135087/1‑149 (MQ=255)
                                                                                                                                              gAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGACCGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTTAGCTGCTGGCCGGGGTGGCGACTAATACCg    >  4:5530/1‑148 (MQ=255)
                                                                                                                                               aGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCa  <  4:133724/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCA  >  NC_000913/435025‑435316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑