Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,434,980 C→T 5.0% T256M (ACG→ATG)  fmt → 10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,434,9800CT 5.0% 254.0 / 2.1 79T256M (ACG→ATG) fmt10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand):  ref base C (40/35);  new base T (2/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01

GTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGTC  >  NC_000913/3434834‑3435126
                                                                                                                                                  |                                                                                                                                                  
gTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACgg                                                                                                                                                  >  4:116761/1‑149 (MQ=255)
      tAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGcaacc                                                                                                                                             >  4:166864/1‑148 (MQ=255)
         agaagaagCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATATGGCAACCAACg                                                                                                                                         >  1:217410/1‑149 (MQ=255)
         agaagaagCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATATGGCAACCAACg                                                                                                                                         >  1:220886/1‑149 (MQ=255)
         agaagaagCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACg                                                                                                                                         >  7:235208/1‑149 (MQ=255)
         agaagaagCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACg                                                                                                                                         >  7:19013/1‑149 (MQ=255)
         agaagaagCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACg                                                                                                                                         <  2:120133/149‑1 (MQ=255)
          gaagaagCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGc                                                                                                                                        <  2:76980/149‑1 (MQ=255)
                gcgcGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGc                                                                                                                                                 <  7:59095/134‑1 (MQ=255)
                gcgcGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGc                                                                                                                                                 >  8:59095/1‑134 (MQ=255)
                         gACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGAt                                                                                                                         >  3:214027/1‑149 (MQ=255)
                           cTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATcc                                                                                                                       <  8:177675/149‑1 (MQ=255)
                               tCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTg                                                                                                                    >  4:42397/1‑148 (MQ=255)
                               tCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGa                                                                                                                   <  7:116693/149‑1 (MQ=255)
                               tCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGa                                                                                                                   <  8:237228/149‑1 (MQ=255)
                                 aCTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAATTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAg                                                                                                                 >  1:207850/1‑149 (MQ=255)
                                 aCTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGaa                                                                                                                  <  5:233518/148‑1 (MQ=255)
                                  cTTTCGGCAGCACAGCTTGAACGCTGCATTCTCGCTTTCAATCCATGGACAATGAGCTGGCTTGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCCACCAACGCTGCACCAGGAACGATACTTGCAga                                                                                                                >  5:32113/1‑148 (MQ=255)
                                      cGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCcaa                                                                                                             >  7:13635/1‑148 (MQ=255)
                                      cGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCcaa                                                                                                             <  3:195686/148‑1 (MQ=255)
                                      cGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCcaa                                                                                                             <  1:122318/148‑1 (MQ=255)
                                      cGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCcaa                                                                                                             <  2:222354/148‑1 (MQ=255)
                                       ggCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGCCAGCCGGTTAAAGTCTGGAAAGCATCGGTCATGGATACGGCAACCAACGCTGCACCAGGCACGATCCTTGAAGCctac                                                                                                            >  3:34910/1‑148 (MQ=255)
                                            cacaGCTTGAACGCTGCATCCGCGCTTTCTATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAacaaacaa                                                                                                      <  2:55987/149‑1 (MQ=255)
                                                  ttGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACATACAAGGCAtt                                                                                                >  1:228460/1‑149 (MQ=255)
                                                     aaCGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCa                                                                                              <  2:207850/148‑1 (MQ=255)
                                                      aCGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAgg                                                                                            <  4:34301/149‑1 (MQ=255)
                                                       cGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAAGGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGt                                                                                           <  5:182287/149‑1 (MQ=255)
                                                       cGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGt                                                                                           >  3:19701/1‑149 (MQ=255)
                                                        gCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGtt                                                                                          >  7:83322/1‑149 (MQ=255)
                                                        gCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGtt                                                                                          <  8:19013/149‑1 (MQ=255)
                                                        gCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGtt                                                                                          <  3:166864/149‑1 (MQ=255)
                                                            cATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGc                                                                                        <  4:19701/147‑1 (MQ=255)
                                                             aTTCGCGCTTTCAATCGATGGGCAATGAGCTGGCTGGAAATTGAAGGAGAGCCGGTTTAAGTCTGGAAAGAATCGGTAATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAAGAAACAAGGCATTCTGGTTGAGAc                                                                                     <  4:214027/149‑1 (MQ=255)
                                                             aTTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGAAAGCCGGTTAAAGTCGGGAAAGCATCGGTCATTGATACGGCAACCACCGCTGCACCAGGAACGAACCTTTAAGCCAACAAACAAGGCATTCAGGTTGCGAc                                                                                     >  4:226617/1‑149 (MQ=255)
                                                              ttCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGa                                                                                      >  3:73439/1‑147 (MQ=255)
                                                              ttCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACt                                                                                    >  5:226022/1‑149 (MQ=255)
                                                              ttCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACt                                                                                    >  4:233447/1‑149 (MQ=255)
                                                                 gcgcTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGt                                                                                 >  4:94562/1‑149 (MQ=255)
                                                                    cTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATATGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGAt                                                                              <  2:217410/149‑1 (MQ=255)
                                                                    cTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATATGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGAt                                                                              <  2:220886/149‑1 (MQ=255)
                                                                    cTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGAt                                                                              >  4:181038/1‑149 (MQ=255)
                                                                     tttCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATg                                                                             <  8:13635/149‑1 (MQ=255)
                                                                       tCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTg                                                                                         <  5:170120/135‑1 (MQ=255)
                                                                       tCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTg                                                                                         >  6:170120/1‑135 (MQ=255)
                                                                         aaTCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCCGGTTGCGACTgg                                                                                  >  3:16450/1‑140 (MQ=255)
                                                                         aaTCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTgg                                                                                  <  4:16450/140‑1 (MQ=255)
                                                                           tCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCaacaaac                                                                                                        >  8:141223/1‑116 (MQ=255)
                                                                           tCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCaacaaac                                                                                                        <  7:141223/116‑1 (MQ=255)
                                                                           tCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATcc                                                                       >  7:44934/1‑149 (MQ=255)
                                                                              aTGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTg                                                                     >  4:184227/1‑148 (MQ=255)
                                                                               tGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGaa                                                                   <  3:42397/149‑1 (MQ=255)
                                                                                   cAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTg                                                               >  1:194504/1‑149 (MQ=255)
                                                                                      tGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGctc                                                            <  2:12703/149‑1 (MQ=255)
                                                                                         gctggctgGAAATTGAAGGACAGACGGGTAAAGTCTGGAAAGAATCGGTCATTGATACTGCATCCAACTCTGGAACATGTACGATCCTTGAAGCCAACATACATTGCTTTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCtctc                                                          <  8:212919/148‑1 (MQ=255)
                                                                                             gctggAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTa                                                      >  7:132539/1‑148 (MQ=255)
                                                                                               tggAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGaa                                                                                                                  <  6:146324/86‑1 (MQ=255)
                                                                                               tggAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGaa                                                                                                                  >  5:146324/1‑86 (MQ=255)
                                                                                               tggAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACaa                                                   >  2:113614/1‑149 (MQ=255)
                                                                                                 gAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCAACTGGTGATGGCATCCTGAACCTGCTCTCGTTACaa                                                   <  3:116761/147‑1 (MQ=255)
                                                                                                  aaaTTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCt                                                >  5:43095/1‑149 (MQ=255)
                                                                                                   aaTTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTg                                               >  7:121722/1‑149 (MQ=255)
                                                                                                    aTTGAAGTACAGCCCCTTAAAGTCTGGAAAGCATCGGTCATTGATACGTCAACAAACTCTGCACCAGGAACGATCCTTGAAGCCAACAAACAACGCATTCAGCTTGCGACTGGTCATGGCATCCTGACCCTCATCTCGTAACAACCGGc                                              >  8:206145/1‑149 (MQ=255)
                                                                                                        aaGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGt                                          <  7:44204/149‑1 (MQ=255)
                                                                                                          ggACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGCACGCTCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGt                                          >  2:164075/1‑147 (MQ=255)
                                                                                                          ggACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTaa                                        <  6:43095/149‑1 (MQ=255)
                                                                                                           gACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGt                                          >  6:180586/1‑146 (MQ=255)
                                                                                                             cAGCCGGTTAAAGTCTGGAAAGCATCGGTCATAGATACGGCCACCAACGCTGCACCAGGAACTATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGCCTGGTCATGGCATCCTGAACCTGCACTCGTTACAACCTCCGGGTAAGa                                      >  1:42784/1‑148 (MQ=255)
                                                                                                              aGCCGTTTAACTTCTTGACACCATCGGTCCAGGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCCTTCAGGGTGCGACTGGTGCTGGCATCCGGAACCTGCTCTCGTTACACCCTGCTGGTacgcaa                                    <  6:32113/149‑6 (MQ=255)
                                                                                                                         gTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGACCCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAgcgc                         >  2:191456/1‑149 (MQ=255)
                                                                                                                         gTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAgcgc                         >  2:16995/1‑149 (MQ=255)
                                                                                                                             ggAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCaa                     <  4:73439/149‑1 (MQ=255)
                                                                                                                              gAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAg                    <  1:16995/149‑1 (MQ=255)
                                                                                                                                 aGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGAcc                 >  3:222982/1‑149 (MQ=255)
                                                                                                                                           aTTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCTTCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAActc       >  7:145693/1‑149 (MQ=255)
                                                                                                                                           aTTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCGCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAActc       >  5:182437/1‑149 (MQ=255)
                                                                                                                                              gATACGGCAACCAACGCTGCACCAGCAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACtctc     <  6:178516/148‑1 (MQ=255)
                                                                                                                                               aTACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCtcg    <  8:83322/148‑1 (MQ=255)
                                                                                                                                                tACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCtcgtc  <  2:194504/149‑1 (MQ=255)
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GTTGAGTAAAGAAGAAGCGCGTATTGACTGGTCACTTTCGGCAGCACAGCTTGAACGCTGCATTCGCGCTTTCAATCCATGGCCAATGAGCTGGCTGGAAATTGAAGGACAGCCGGTTAAAGTCTGGAAAGCATCGGTCATTGATACGGCAACCAACGCTGCACCAGGAACGATCCTTGAAGCCAACAAACAAGGCATTCAGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGTC  >  NC_000913/3434834‑3435126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑