Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,884,401 G→A 5.0% M22I (ATG→ATA)  rpmH → 50S ribosomal subunit protein L34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,884,4010GA 5.0% 255.4 / 2.2 80M22I (ATG→ATA) rpmH50S ribosomal subunit protein L34
Reads supporting (aligned to +/- strand):  ref base G (36/39);  new base A (2/2);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

CGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTC  >  NC_000913/3884260‑3884546
                                                                                                                                             |                                                                                                                                                 
cGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGctac                                                                                                                                              <  7:121433/147‑1 (MQ=255)
       cATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATgg                                                                                                                                     >  6:83287/1‑149 (MQ=255)
       cATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTACGGCTACTAAAAATgg                                                                                                                                     >  4:216769/1‑149 (MQ=255)
        aTCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGgt                                                                                                                                    >  8:229384/1‑149 (MQ=255)
            tttAATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTTTACTGAAGCGAAACCGTTCTCACGGCTTCCGTGCTAGTATGGCTACTAAAAATGgtcct                                                                                                                                 >  4:13519/1‑146 (MQ=255)
              taaTTTTTCGGCGGATATACATTAAAGCCATTGAATTTATTCAAGTTTAGGTTGAACTCGGCATGAAACTCACTTTTCAACCGTCTGTACTGAAGCGCTACGGTTCTCACGGCTTGCGTGCTCGTATGGCTTCTAAAAATGGTCGTCa                                                                                                                               <  1:35242/146‑1 (MQ=255)
                 tttttCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGtt                                                                                                                           >  8:28201/1‑149 (MQ=255)
                   tttCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTc                                                                                                                          <  4:136462/148‑1 (MQ=255)
                      cgtctgATATCCAATAAAGCCATTGAATTTATTCAAGTTTATGTAGAAATCTCAATGAAACGCACTTTTCAACCTTATGTACTTAAGCTCAACCGTTCTCACGGCTTCCTTGATCGTATTGCTACTAAAAATGGTCGTCAGGTTCTgg                                                                                                                       <  7:173440/143‑1 (MQ=255)
                      cggcggATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTgg                                                                                                                       <  5:153845/148‑1 (MQ=255)
                       ggcggATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGc                                                                                                                      <  2:82361/148‑1 (MQ=255)
                       ggcggATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCa                                                                                                                     >  1:211575/1‑149 (MQ=255)
                               tCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTAGGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgtcgtc                                                                                                              <  4:155436/148‑1 (MQ=255)
                                ccAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCAcgtcgtcgt                                                                                                            >  3:191655/1‑149 (MQ=255)
                                      aaGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACTCACTTTTCAACCTTCTGTACTGAAGCGCAACCGTTCTCCCGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTa                                                                                                        >  4:24727/1‑147 (MQ=255)
                                      aaGCCATTGAATTTAATCAAGTTTAGGTAGAAATCGCCATGATACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTaaa                                                                                                      <  4:2308/149‑1 (MQ=255)
                                       aGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAg                                                                                                     >  5:191131/1‑149 (MQ=255)
                                         ccATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGc                                                                                                   >  6:7601/1‑149 (MQ=255)
                                           aTTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTaaa                                                                                                      >  7:214815/1‑144 (MQ=255)
                                           aTTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTaaa                                                                                                      <  8:214815/144‑1 (MQ=255)
                                            ttGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCcgc                                                                                                <  2:157067/149‑1 (MQ=255)
                                            ttGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCcgc                                                                                                >  7:38880/1‑149 (MQ=255)
                                               aaTTTATTCAAGTTTAGGTAGAAATAGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCt                                                                                             >  5:155539/1‑149 (MQ=255)
                                                aTTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTc                                                                                            >  8:169641/1‑149 (MQ=255)
                                                     ttCAAGTTTAGGTAGAACTCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCt                                                                                        <  4:11095/148‑1 (MQ=255)
                                                     ttCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCt                                                                                        <  2:229412/148‑1 (MQ=255)
                                                     ttCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCt                                                                                        >  1:225944/1‑148 (MQ=255)
                                                        aaGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGAcc                                                                                    <  5:83287/149‑1 (MQ=255)
                                                        aaGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGAcc                                                                                    <  7:28201/149‑1 (MQ=255)
                                                         aGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCg                                                                                   <  8:94524/149‑1 (MQ=255)
                                                          gTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGt                                                                                  <  1:15087/149‑1 (MQ=255)
                                                            ttAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGttt                                                                                >  8:34988/1‑149 (MQ=255)
                                                               ggTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCACACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCCCTCGTCTGACCGATTCt                                                                              >  1:172624/1‑148 (MQ=255)
                                                                 tAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAg                                                                           <  2:211575/149‑1 (MQ=255)
                                                                  aGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAg                                                                           >  6:134817/1‑148 (MQ=255)
                                                                       tCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGtaataa                                                                     <  2:225944/149‑1 (MQ=255)
                                                                       tCGCCATGAAACGCACTTTTAAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGGCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGtaataa                                                                     <  7:219006/149‑1 (MQ=255)
                                                                         gCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGGTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATaaa                                                                    <  6:155539/148‑1 (MQ=255)
                                                                         gCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTACGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAg                                                                   <  3:216769/149‑1 (MQ=255)
                                                                             tGAAACGCACTTTTCCACCGTCTGTACTGAGTCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTaa                                                               >  6:216076/1‑149 (MQ=255)
                                                                             tGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTaa                                                               <  6:191131/149‑1 (MQ=255)
                                                                              gAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAAc                                                              >  3:134798/1‑149 (MQ=255)
                                                                                aaCGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATAGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAAccc                                                            >  4:108896/1‑149 (MQ=255)
                                                                                aaCGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATAGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAAccc                                                            >  1:73807/1‑149 (MQ=255)
                                                                                 aCGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAAcccc                                                           >  7:89072/1‑149 (MQ=255)
                                                                                   gCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCt                                                          >  7:13428/1‑148 (MQ=255)
                                                                                       ttttCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTg                                                     >  1:216554/1‑149 (MQ=255)
                                                                                         ttCAACCGTCTGTCCTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGGCGGCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTgg                                                    <  6:145347/148‑1 (MQ=255)
                                                                                         ttCAACCGTCTGTACTGAAGCGCAACCGTTCTCACTGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGt                                                   >  1:164148/1‑149 (MQ=255)
                                                                                            aaCCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTaa                                                <  7:34988/149‑1 (MQ=255)
                                                                                            aaCCGTCTGTACTGAAGCGCAACAGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTaa                                                >  2:121003/1‑149 (MQ=255)
                                                                                                gTCTGTACTTAAGCGCTACCGTTCTCCCGGCGTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTc                                            <  7:229384/149‑1 (MQ=255)
                                                                                                     tACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCAtt                                       >  1:206751/1‑149 (MQ=255)
                                                                                                     tACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCAt                                        <  1:22020/148‑1 (MQ=255)
                                                                                                        tGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTcc                                    >  7:81512/1‑149 (MQ=255)
                                                                                                        tGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTcc                                    >  5:233839/1‑149 (MQ=255)
                                                                                                        tGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTcc                                    >  2:79506/1‑149 (MQ=255)
                                                                                                        tGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTcc                                    >  8:93938/1‑149 (MQ=255)
                                                                                                         gAAGCGCACCCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGGTAAGCTCGCATTTcc                                    >  1:207652/1‑148 (MQ=255)
                                                                                                         gAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTcc                                    >  8:142534/1‑148 (MQ=255)
                                                                                                          aaGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTc                                     >  3:132789/1‑146 (MQ=255)
                                                                                                          aaGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTc                                     <  4:132789/146‑1 (MQ=255)
                                                                                                            gcgcAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAgg                                <  8:89072/149‑1 (MQ=255)
                                                                                                              gcAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGa                              <  2:85798/149‑1 (MQ=255)
                                                                                                              gcAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTAATAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGa                              <  5:86641/149‑1 (MQ=255)
                                                                                                                 aCCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGtt                           <  1:134534/149‑1 (MQ=255)
                                                                                                                   cGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTAc                         <  7:169641/149‑1 (MQ=255)
                                                                                                                        tcACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTg                    >  7:50580/1‑149 (MQ=255)
                                                                                                                          aCGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGGCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGtt                  <  2:98673/149‑1 (MQ=255)
                                                                                                                          aCGGCTTCCGTGCTCGTATAGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCtt                     <  2:73807/146‑1 (MQ=255)
                                                                                                                          aCGGCTTCCGTGCTCGTATAGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCtt                     <  3:108896/146‑1 (MQ=255)
                                                                                                                             gCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTATCCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAAc               <  5:216076/149‑1 (MQ=255)
                                                                                                                               ttCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGt                                                      >  8:135787/1‑108 (MQ=255)
                                                                                                                               ttCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGt                                                      <  7:135787/108‑1 (MQ=255)
                                                                                                                                 ccGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCGCTGAGTGGTTAAGCTGGCATTTCCCAGGGAGTTACGCTTGTTAACTcc            <  2:164148/148‑1 (MQ=255)
                                                                                                                                 ccGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTcc            <  8:13428/148‑1 (MQ=255)
                                                                                                                                    tGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGt        <  2:216554/149‑1 (MQ=255)
                                                                                                                                    tGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGt        <  5:134817/149‑1 (MQ=255)
                                                                                                                                       tCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCaa     >  7:96503/1‑149 (MQ=255)
                                                                                                                                          tATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTc  <  8:50580/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
CGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTC  >  NC_000913/3884260‑3884546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑