Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,929,682 A→C 5.1% L471R (CTG→CGG)  ravA ← hexameric AAA+ MoxR family ATPase, putative molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,929,6820AC 5.1% 293.2 / 3.8 92L471R (CTG→CGG) ravAhexameric AAA+ MoxR family ATPase, putative molecular chaperone
Reads supporting (aligned to +/- strand):  ref base A (42/43);  new base C (3/2);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAAC  >  NC_000913/3929540‑3929830
                                                                                                                                              |                                                                                                                                                    
tctcTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACAtt                                                                                                                                                >  3:99211/1‑149 (MQ=255)
   ctTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACAttttt                                                                                                                                             <  6:70015/149‑1 (MQ=255)
    ttCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTg                                                                                                                                            >  3:205501/1‑149 (MQ=255)
         tCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACCGACATTTTTGCTGtt                                                                                                                                       >  4:182061/1‑149 (MQ=255)
         tCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACCGACATTTTTGCTGtt                                                                                                                                       >  4:178849/1‑149 (MQ=255)
           aaTCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTc                                                                                                                                      <  5:217530/148‑1 (MQ=255)
           aaTCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCg                                                                                                                                     >  5:144985/1‑149 (MQ=255)
             tCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTc                                                                                                                                      <  2:231283/146‑1 (MQ=255)
             tCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTc                                                                                                                                      >  1:231283/1‑146 (MQ=255)
              cccTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCt                                                                                                                                   >  3:19631/1‑148 (MQ=255)
                ctctTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGaa                                                                                                                                <  4:99211/149‑1 (MQ=255)
                ctctTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGaa                                                                                                                                >  4:217861/1‑149 (MQ=255)
                   ttCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAAt                                                                                                                               <  3:202567/147‑1 (MQ=255)
                          aCGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCgt                                                                                                                        <  5:144854/147‑1 (MQ=255)
                           cGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGtt                                                                                                                     <  7:229969/149‑1 (MQ=255)
                            ggCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGttg                                                                                                                    <  1:233378/149‑1 (MQ=255)
                               cAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACCGACATTTTTGCTGTTCGCTGAATAAAGCGTGttgctt                                                                                                                 >  5:103078/1‑149 (MQ=255)
                               cAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACCGACATTTTTGCTGTTCGCTGAATAAAGCGTGttgctt                                                                                                                 <  3:182061/149‑1 (MQ=255)
                               cAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACCGACATTTTTGCTGTTCGCTGAATAAAGCGTGttgctt                                                                                                                 <  3:178849/149‑1 (MQ=255)
                               cAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGttgctt                                                                                                                 <  1:238589/149‑1 (MQ=255)
                                aGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGttgcttg                                                                                                                <  3:135848/149‑1 (MQ=255)
                                aGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGttgcttg                                                                                                                <  5:61496/149‑1 (MQ=255)
                                   agcacaTTAAGCGTATCCAGCGTTATCTTTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGTGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTgcgc                                                                                                             <  3:6644/147‑1 (MQ=255)
                                    tCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTgcgc                                                                                                             >  1:205433/1‑148 (MQ=255)
                                    tCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTgcgc                                                                                                             >  5:94850/1‑148 (MQ=255)
                                     cacaTTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCgtgt                                                                                                                      >  5:69399/1‑138 (MQ=255)
                                     cacaTTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCgtgt                                                                                                                      <  6:69399/138‑1 (MQ=255)
                                        aTTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTc                                                                                                        >  6:115257/1‑149 (MQ=255)
                                        aTTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTc                                                                                                        >  1:182496/1‑149 (MQ=255)
                                        aTTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTc                                                                                                        >  5:164029/1‑149 (MQ=255)
                                             gCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGt                                                                                                                                        >  1:100926/1‑112 (MQ=255)
                                             gCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGt                                                                                                                                        <  2:100926/112‑1 (MQ=255)
                                              cGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATCCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTc                                                                                                  <  2:190716/149‑1 (MQ=255)
                                                tATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGTCAGTCGCTTTCaa                                                                                                <  4:19631/149‑1 (MQ=255)
                                                  tCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTgcg                                                                                                              >  5:77251/1‑133 (MQ=255)
                                                  tCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTgcg                                                                                                              <  6:77251/133‑1 (MQ=255)
                                                    cAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGctc                                                                                            <  5:115257/149‑1 (MQ=255)
                                                    cAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGct                                                                                             <  4:154058/148‑1 (MQ=255)
                                                             ctttgttgTGCCTGGAGAATCTGTGCACCGACATCCTGTAGTCTGGCTTCAATGCGACCTAACCAGTCGCCAGTGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAgttg                                                                                   <  1:94433/147‑1 (MQ=255)
                                                             cATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACGGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAgttg                                                                                   >  5:211623/1‑149 (MQ=255)
                                                               ttgttgTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAgttgtt                                                                                 >  1:133655/1‑149 (MQ=255)
                                                                 gttgtGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAgttgttg                                                                                <  2:182496/148‑1 (MQ=255)
                                                                  ttgtGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAgttgttgtt                                                                              <  7:6435/149‑1 (MQ=255)
                                                                  ttgtGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGGCGCTTTCAAGCTCTTCCAgttgttgtt                                                                              <  2:177793/149‑1 (MQ=255)
                                                                     tgCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGtttt                                                                            <  2:205433/148‑1 (MQ=255)
                                                                      gCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTAt                                                                          >  3:38686/1‑149 (MQ=255)
                                                                        cTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGCCCTAACCAGTCGCCAGGGATAAACAGCCATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTAttt                                                                        >  1:104557/1‑149 (MQ=255)
                                                                        cTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAgttg                                                                                   >  5:50575/1‑138 (MQ=255)
                                                                        cTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAgttg                                                                                   <  6:50575/138‑1 (MQ=255)
                                                                        cTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTa                                                                           >  5:74640/1‑146 (MQ=255)
                                                                        cTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTa                                                                           <  6:74640/146‑1 (MQ=255)
                                                                         tGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGc                                                                                              >  5:38297/1‑126 (MQ=255)
                                                                         tGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGc                                                                                              <  6:38297/126‑1 (MQ=255)
                                                                         tGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTAttt                                                                        >  8:208101/1‑148 (MQ=255)
                                                                          ggCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGCATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCa                                                                      >  7:146039/1‑149 (MQ=255)
                                                                             gAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCAc                                                                     >  7:89873/1‑147 (MQ=255)
                                                                             gAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCa                                                                   <  7:208101/149‑1 (MQ=255)
                                                                                           aCATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGc                                                     <  6:6490/149‑1 (MQ=255)
                                                                                             aTCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGcc                                                    >  2:46717/1‑148 (MQ=255)
                                                                                              tCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGa                                                  <  8:67010/149‑1 (MQ=255)
                                                                                                cTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGAt                                                 <  8:89873/148‑1 (MQ=255)
                                                                                                 tGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATg                                                >  1:195454/1‑148 (MQ=255)
                                                                                                     ggctggctTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCg                                           >  8:213088/1‑149 (MQ=255)
                                                                                                     ggctggctTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTCGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCg                                           >  2:52614/1‑149 (MQ=255)
                                                                                                         ggctTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGttgct                                                                                                                  <  7:189578/74‑1 (MQ=255)
                                                                                                         ggctTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGttgct                                                                                                                  >  8:189578/1‑74 (MQ=255)
                                                                                                          gctTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGaa                                       <  3:217861/148‑1 (MQ=255)
                                                                                                            ttCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCCGCCGATGAACCGGGAAGTg                                    >  2:67011/1‑149 (MQ=255)
                                                                                                            ttCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTg                                    >  3:66842/1‑149 (MQ=255)
                                                                                                               aaTGCGCACTAACCAGTCGCCAGGGATAATCAGACATTTTTGCTGTTCGCTGAATATAGCGTGTTGCTTGCTACAGTCGCTTTAAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCAGATGAACCGGGAAGTGCCa                                 <  4:288/149‑1 (MQ=255)
                                                                                                                aTGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACTTTCAGCCGATGAACCGGGAAGTGCCAg                                >  1:160007/1‑149 (MQ=255)
                                                                                                                 tGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGc                               >  7:162699/1‑149 (MQ=255)
                                                                                                                    gACCTACCCAGAAGCCACGGATAAACAGACATTTTTTCAGTTCGCTCAATAAAGCGAGTTGATAGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACAATGCAGACCTTCAGCCGATTAACAGGGAAGTGCCAGCga                             >  8:215578/1‑147 (MQ=255)
                                                                                                                    gACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTa                            <  5:197533/149‑1 (MQ=255)
                                                                                                                    gACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTa                            <  2:195454/149‑1 (MQ=255)
                                                                                                                     aCCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTAc                           >  7:143394/1‑149 (MQ=255)
                                                                                                                         aaCCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAg                                                                                               <  3:139902/77‑1 (MQ=255)
                                                                                                                         aaCCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAg                                                                                               >  4:139902/1‑77 (MQ=255)
                                                                                                                          aCCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCt                      >  6:19029/1‑149 (MQ=255)
                                                                                                                           ccAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCtt                     <  3:83028/149‑1 (MQ=255)
                                                                                                                                    aGGGATAAACGGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCTGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTa            <  6:211623/149‑1 (MQ=255)
                                                                                                                                       gATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCg                                           <  4:185546/115‑1 (MQ=255)
                                                                                                                                       gATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCg                                           >  3:185546/1‑115 (MQ=255)
                                                                                                                                       gATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACAt         >  5:138770/1‑149 (MQ=255)
                                                                                                                                         tAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATcg       <  7:164949/149‑1 (MQ=255)
                                                                                                                                           aaCAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTAGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATcgc      >  1:99796/1‑148 (MQ=255)
                                                                                                                                            aCAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCa                                 <  6:143400/120‑1 (MQ=255)
                                                                                                                                            aCAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCa                                 >  5:143400/1‑120 (MQ=255)
                                                                                                                                            aCAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCtt                     <  6:231664/132‑1 (MQ=255)
                                                                                                                                            aCAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCtt                     >  5:231664/1‑132 (MQ=255)
                                                                                                                                             cAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACTTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGaa   <  2:160007/149‑1 (MQ=255)
                                                                                                                                             cAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGaa   >  7:17452/1‑149 (MQ=255)
                                                                                                                                              aGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAAc  >  2:48882/1‑149 (MQ=255)
                                                                                                                                              aGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAAc  <  5:133728/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
TCTCTTCGATCAATCCCTCTTCGCTGACGGCCAGCATCACATTAAGCGTATCCAGCGTTAGCATTGTTGTGCCTGGCGAATCTGTGCACCGACATCCTGTAGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAAC  >  NC_000913/3929540‑3929830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: