Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,590,647 | G→C | 6.4% | Y211* (TAC→TAG) | yjiY ← | putative transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,590,647 | 0 | G | C | 6.4% | 206.5 / 5.5 | 63 | Y211* (TAC→TAG) | yjiY | putative transporter |
Reads supporting (aligned to +/- strand): ref base G (32/26); new base C (2/2); total (35/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01 |
GAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAG > NC_000913/4590500‑4590792 | gAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATg > 2:87011/1‑148 (MQ=255) gTAATGGTGGTGTCTTTAAAGGTCAGTGCCGTACCCCAGTACGGATCCTGCGCAATCACGCCACCGAAGTAGATAGATGCAACCAGCAGCACGATACCAATGACAGAGACTTCCCCCCCACGCCCCGGATGAATAAAGCGCATGTaca > 1:41753/1‑146 (MQ=255) gTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGa > 8:231326/1‑148 (MQ=255) ttggtgTCTTTAAAGGTAAGAGCCGGACCCCAGAACGGATCCTGAGCAATCACGCCACCGAAGAAGATAGAGGCAACCAGCAGCACGAAACCAATCACAGATACTTCAACCACACGCCCCCCACGAAAAAATCGCATGTAGAAAcaca > 8:74664/2‑148 (MQ=255) gtggtgTATTTAATGGTCAGTTCCGGACCCCAGTACGGATCGTTAGCAATCACGCCACCGAATTAGATAGAGGAAACCAGCAGCAAGATAACACTGACAGAGACTTCACCCACACGACCCGGACGAATAAAGCGCATGTAGATACCCAt < 8:229548/149‑1 (MQ=255) tCTTTTAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCCCAGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGATACTTCACCCACACGCCCCGGATGAATAAAGCGCATGTAGATACCCATAAACa < 2:41753/148‑1 (MQ=255) ttAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAgcg > 3:207015/1‑148 (MQ=255) tAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAgc < 4:32231/146‑1 (MQ=255) tCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCGATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAgc > 6:215535/1‑140 (MQ=255) tCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCGATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAgc < 5:215535/140‑1 (MQ=255) cAGTGCCGGCCCCCAGTCCGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATCCCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTa < 2:152225/149‑1 (MQ=255) cAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTa > 5:61361/1‑149 (MQ=255) aGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTa < 8:9455/148‑1 (MQ=255) ggACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGa < 7:231326/149‑1 (MQ=255) gACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAg < 6:61361/149‑1 (MQ=255) cAGTACGGATCGTGCGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAAc > 2:115963/1‑149 (MQ=255) cAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCACCCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAAc > 3:40263/1‑149 (MQ=255) aGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACg > 7:186962/1‑149 (MQ=255) aGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACg > 7:133918/1‑149 (MQ=255) tACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGt < 7:74664/149‑1 (MQ=255) ggATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACCAATAAAGCGCATTTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTg > 5:100576/1‑147 (MQ=255) gAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACAccc > 1:40547/1‑149 (MQ=255) gCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCa > 5:107178/1‑149 (MQ=255) aCGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCCCACGCCCCGGCCGAATAACGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGATGAGCAACAGGTGAAGACCCCCCACGGACt > 2:178154/1‑149 (MQ=255) gCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACCCGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACttt > 4:110210/1‑149 (MQ=255) ccGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGc < 1:87011/149‑1 (MQ=255) atagaGGCAACCAGCAGCACGATACCAATGACAGAGCCTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCtt > 2:135846/1‑148 (MQ=255) atagaGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCttt < 1:115963/149‑1 (MQ=255) atagaGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCtt > 8:90070/1‑148 (MQ=255) atagaGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGATTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCttt < 3:9690/149‑1 (MQ=255) gagGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTaa < 7:144343/148‑1 (MQ=255) ggCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAgg > 8:124889/1‑139 (MQ=255) ggCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAgg < 7:124889/139‑1 (MQ=255) aaCCAGCAGCGCGATACCAATGACAGAGACATCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAAAAGCGCAATCGGTACGGTTGAGCAAAAGGTGAAGACACCCCACGGACTTTCGGCCAGGGATTTAACCACaa > 8:183963/1‑149 (MQ=255) gATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGa > 5:84465/1‑149 (MQ=255) cAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGc > 6:138336/1‑148 (MQ=255) cAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGc > 6:15511/1‑148 (MQ=255) cAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTACATACCCATAAACACCGCAATCGCAACGGTACAGCAAACGGTGAAGACACCCCACGGACTTTCGTCCAGGGCTTTAACCACAATCACCGCCAGGACGGc > 1:219950/1‑148 (MQ=255) aaTGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTa < 7:95708/124‑1 (MQ=255) aaTGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTa > 8:95708/1‑124 (MQ=255) aaTGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATCTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCg > 8:142047/1‑112 (MQ=255) aaTGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATCTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCg > 8:145514/1‑112 (MQ=255) aaTGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATCTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCg < 7:142047/112‑1 (MQ=255) aaTGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATCTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCg < 7:145514/112‑1 (MQ=255) aTGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAg > 2:41460/1‑149 (MQ=255) tGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAgg < 5:138336/149‑1 (MQ=255) agagACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTg > 4:12948/1‑85 (MQ=255) agagACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTg < 3:12948/85‑1 (MQ=255) tCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCAt > 7:52334/1‑149 (MQ=255) aCCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGa < 6:84465/149‑1 (MQ=255) ccACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAACCCGCGCAATCGGTACGGTTGCGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTGTAACCACAATCAGCGCCCGGACGGCGAGGATGATGATCATGAt > 3:140467/1‑148 (MQ=255) ccACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGCGCAAACGGTGAAGCCACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGAt > 5:78654/1‑148 (MQ=255) ccACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGTTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGAt > 1:143402/1‑148 (MQ=255) acacGCCCCGGTCGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGGTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTTACCACAATAAGCGCCAGGACGGCGAGGATGATGATCATGATTaa < 8:186962/149‑1 (MQ=255) acacGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCAAGGACGGCGAGGATGATGATCATGAtt > 2:105627/1‑147 (MQ=255) cccGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACa < 7:90070/149‑1 (MQ=255) ccGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAg < 1:41460/149‑1 (MQ=255) cGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGc < 5:15511/149‑1 (MQ=255) cGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGc < 4:40263/149‑1 (MQ=255) aCGAATAATGCGCATGTTGATACCCATAAACAGCTCAATCGGTACGGTTGAGCAAACTGTGAAGACAACCCACTGTCTTTCGGGCAGGGCTTTAAGCACAATCAGCGCCAGGTCGGCGAGGATGATGATCATGTTTAAGATACAGCCaa < 6:100576/149‑1 (MQ=255) gcgcATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGa < 6:107178/149‑1 (MQ=255) cATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAg > 8:168702/1‑149 (MQ=255) aTGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCttt < 2:216111/80‑1 (MQ=255) aTGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCttt > 1:216111/1‑80 (MQ=255) | GAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAGAGGCAACCAGCAGCACGATACCAATGACAGAGACTTCACCCACACGCCCCGGACGAATAAAGCGCATGTAGATACCCATAAACAGCGCAATCGGTACGGTTGAGCAAACGGTGAAGACACCCCACGGACTTTCGGCCAGGGCTTTAACCACAATCAGCGCCAGGACGGCGAGGATGATGATCATGATTAAGAAACAGCCAAACAGCGCGATAG > NC_000913/4590500‑4590792 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |