Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 580,020 G→A 7.1% R47R (AGG→AGA)  ybcW → DLP12 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,0200GA 7.1% 212.2 / 5.3 71R47R (AGG→AGA) ybcWDLP12 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (28/37);  new base A (2/3);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

CGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGG  >  NC_000913/579873‑580164
                                                                                                                                                   |                                                                                                                                                
cGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAggg                                                                                                                                                 <  2:96232/149‑1 (MQ=255)
       aTGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGa                                                                                                                                          <  7:52858/149‑1 (MQ=255)
           aTAAAGCACAATCTGCTGATGATCCCTCCGTTGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCa                                                                                                                                      <  1:241208/149‑1 (MQ=255)
              aaGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCg                                                                                                                                   >  5:14413/1‑149 (MQ=255)
               aGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAggg                                                                                                                                                 >  6:240337/1‑134 (MQ=255)
               aGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAggg                                                                                                                                                 <  5:240337/134‑1 (MQ=255)
                 aaCAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTg                                                                                                                                <  7:75527/149‑1 (MQ=255)
                  aCAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTgg                                                                                                                               <  7:239497/149‑1 (MQ=255)
                   cAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGt                                                                                                                              <  1:172145/149‑1 (MQ=255)
                    aaTCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTgg                                                                                                                               >  8:181280/1‑147 (MQ=255)
                    aaTCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTgg                                                                                                                               <  7:181280/147‑1 (MQ=255)
                    aaTCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGt                                                                                                                              >  3:112781/1‑148 (MQ=255)
                      tCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACACTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTg                                                                                                                             >  5:54638/1‑147 (MQ=255)
                       ctgctgATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAg                                                                                                                          >  8:241150/1‑149 (MQ=255)
                            gatgatCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAAGTGAGGCAGCGTTGGTGAAGCACGa                                                                                                                     >  3:46827/1‑149 (MQ=255)
                             atgatCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGat                                                                                                                    <  7:187531/149‑1 (MQ=255)
                                 tCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGataata                                                                                                                <  8:230959/149‑1 (MQ=255)
                                 tCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGataata                                                                                                                <  6:207811/149‑1 (MQ=255)
                                   ccTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATATAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGataataa                                                                                                               >  8:180839/1‑148 (MQ=255)
                                    cTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATCGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGataataata                                                                                                             <  6:107225/149‑1 (MQ=255)
                                    cTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGataataata                                                                                                             <  8:70782/149‑1 (MQ=255)
                                       cGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGa                                                                                                          <  1:82682/149‑1 (MQ=255)
                                        gTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGaa                                                                                                         <  5:173574/149‑1 (MQ=255)
                                         tGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGATGAAGCACGATAATAATATGAAg                                                                                                        <  8:217661/149‑1 (MQ=255)
                                                gATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGattatt                                                                                                 >  7:206224/1‑149 (MQ=255)
                                                 aTTAGTGTAAAAAATATGATTAATAGCACCATTTCTATGAGTTACCATGATGCTGTAATTGCATGTATAGAAAATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGat                                                                                                     <  2:24676/144‑1 (MQ=255)
                                                   tCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTcc                                                                                               <  6:14413/148‑1 (MQ=255)
                                                     gtgtAAAAAATATGCTTAATAGCACCATTTCTTTGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCtg                                                                                            <  5:55138/149‑1 (MQ=255)
                                                         aaaaaaTATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCtggtgg                                                                                        >  4:72847/1‑149 (MQ=255)
                                                                   cTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGAt                                                                               <  7:241150/148‑1 (MQ=255)
                                                                    ttAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTg                                                                                                                                <  6:144863/98‑1 (MQ=255)
                                                                    ttAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTg                                                                                                                                >  5:144863/1‑98 (MQ=255)
                                                                      aaTAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCAc                                                                            >  4:118669/1‑148 (MQ=255)
                                                                            aCCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACt                                                                     >  2:74092/1‑149 (MQ=255)
                                                                             ccATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTg                                                                    >  6:83614/1‑149 (MQ=255)
                                                                               aTTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCtgg                                                                                           <  8:178585/124‑1 (MQ=255)
                                                                               aTTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCtgg                                                                                           >  7:178585/1‑124 (MQ=255)
                                                                               aTTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGc                                                                   >  3:115070/1‑148 (MQ=255)
                                                                                tttCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCt                                                                  >  6:202715/1‑148 (MQ=255)
                                                                                   cTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGa                                                                                                      <  5:26794/109‑1 (MQ=255)
                                                                                   cTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGa                                                                                                      >  6:26794/1‑109 (MQ=255)
                                                                                        aGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGAATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTc                                                          <  2:123949/148‑1 (MQ=255)
                                                                                          ttACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCaaa                                                       <  6:54638/149‑1 (MQ=255)
                                                                                            aCCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGTCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAac                                                      <  6:205439/148‑1 (MQ=255)
                                                                                              ccTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAactac                                                   >  7:94537/1‑149 (MQ=255)
                                                                                              ccTGATGTGGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAactac                                                   <  6:181993/149‑1 (MQ=255)
                                                                                               cTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAactatt                                                  <  1:227325/149‑3 (MQ=255)
                                                                                                   tgttgtAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAAc                                              <  8:206224/149‑1 (MQ=255)
                                                                                                    gttgtAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAAc                                              <  5:83614/148‑1 (MQ=255)
                                                                                                       gtAAATGCAACTATAGAACATAAGGTGACTCTGGAAGCATACAGCGCAATTGAGGCAGCGTTGGTGCAGCACGATAATAATATGAAGGATAATTCCCAGGTCGTTGACTGATCACCATAACTGATAATCATTCAAACTACTTAACCagt                                          >  8:121275/1‑149 (MQ=255)
                                                                                                        tAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCtgtg                                         <  4:115070/149‑1 (MQ=255)
                                                                                                         aaTTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCtgtg                                         >  3:68465/1‑148 (MQ=255)
                                                                                                               aTGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGc                                  <  8:178838/149‑1 (MQ=255)
                                                                                                                tGTATATAACATAAGGTGTCTCTTGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAATGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGcc                                 <  5:183148/149‑1 (MQ=255)
                                                                                                                 gTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGcc                                 >  3:2132/1‑148 (MQ=255)
                                                                                                                   ataGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCaa                               >  3:206798/1‑148 (MQ=255)
                                                                                                                    taGATCATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAaca                             >  6:48911/1‑149 (MQ=255)
                                                                                                                    taGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGTCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCATGGAGGTTGAATGATCACAATAAATGCTAATCATTCAAACTACTTACACTGTGACAGAGacaaca                             >  8:62033/1‑149 (MQ=255)
                                                                                                                    taGAACATAAGGTGTCTCTGGAAGCATACACGCCAAATGACGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTCGTCGTTGACTGATCACCATAACTCCTAATCATTCAAACTACATAACCTGTGACAGAGCCAaca                             >  8:65547/1‑149 (MQ=255)
                                                                                                                        aCATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACg                           >  7:182798/1‑147 (MQ=255)
                                                                                                                           tAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTc                      >  7:206899/1‑149 (MQ=255)
                                                                                                                               gtgtCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTctgt                   >  4:82827/1‑148 (MQ=255)
                                                                                                                                tgtCTCTGGAAGCATTCACGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTAATCCCTGGTGGTTTACTCATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACACAGCCAACACGCAGTctgtca                 <  4:526/149‑1 (MQ=255)
                                                                                                                                  tctcTTGAAGCATTCAGGGCAATTGAGGCATCGTTGGTGAAGCACGATAATAATATGAATGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTctgtcact               <  8:56641/149‑1 (MQ=255)
                                                                                                                                  tctcTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTctgtcact               <  5:202715/149‑1 (MQ=255)
                                                                                                                                    tctGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTctgtcactgt             >  4:178978/1‑149 (MQ=255)
                                                                                                                                    tctGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTctgtcactgt             >  6:51254/1‑149 (MQ=255)
                                                                                                                                    tctGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTctgtcactgt             >  5:210847/1‑149 (MQ=255)
                                                                                                                                      tGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACAATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTctgtcactgt             <  4:68465/147‑1 (MQ=255)
                                                                                                                                        gAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTctgtcactgtca           <  8:41449/147‑1 (MQ=255)
                                                                                                                                           gCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGaaa      <  4:2132/149‑1 (MQ=255)
                                                                                                                                              ttCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTg   <  8:94537/149‑1 (MQ=255)
                                                                                                                                              ttCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTg   <  7:180839/149‑1 (MQ=255)
                                                                                                                                                cAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTgg  <  4:112781/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGG  >  NC_000913/579873‑580164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑