Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 792,922 C→T 7.8% W289* (TGG→TGA)  modF ← molybdate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913792,9220CT 7.8% 152.0 / 3.0 51W289* (TGG→TGA) modFmolybdate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (26/21);  new base T (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAG  >  NC_000913/792779‑793065
                                                                                                                                               |                                                                                                                                               
tGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAgtg                                                                                                                                            <  5:137512/149‑1 (MQ=255)
  cTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAgtg                                                                                                                                            <  2:245101/147‑1 (MQ=255)
  cTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAgtg                                                                                                                                            >  1:245101/1‑147 (MQ=255)
        gATATCCCAGAGGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGGGGTAACCGTGCGGATGATCGCCAGTAACCAGGCTTAAAAACGGCGATTATCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCt                                                                                                                                     <  5:39135/148‑1 (MQ=255)
            tCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGTCAGTGTTCGCCTGGAtt                                                                                                                                >  2:164541/1‑149 (MQ=255)
            tCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCGTAATAACGTCGATATTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGCAtt                                                                                                                                >  1:228086/1‑149 (MQ=255)
               cAGATGGTATCGCCGCTCCTCCGACCTCGTCCGAAAAGCGACAACTCGTTTCTGTAACCTAGCGGATGATCGCCAGTAACCAGCCTTAAGAACGTCGATTTTCCAGCACCAATTGGCCCGACAAATACCCAGTGTTATCCTGGATTCAc                                                                                                                             >  2:218331/1‑149 (MQ=255)
                     gTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGc                                                                                                                         >  4:216955/1‑147 (MQ=255)
                        tCGCCGCTGCAGCGACGACGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGTCCCGACAATTTGCCAGTGTTAGACTGGATTCACCTGCCAGc                                                                                                                     >  8:184188/1‑148 (MQ=255)
                                 ccGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGttatt                                                                                                           <  4:184491/149‑1 (MQ=255)
                                     gACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGAGTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGAGTCACCTGCCAGCTAAGGTTATTTAg                                                                                                        >  5:72751/1‑148 (MQ=255)
                                       cgtcgtCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAAt                                                                                                     <  1:190124/149‑1 (MQ=255)
                                        gtcttcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGTCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATg                                                                                                    <  1:164541/149‑1 (MQ=255)
                                           gtcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATggg                                                                                                  <  3:60772/148‑1 (MQ=255)
                                               gAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGAt                                                                                             >  7:87280/1‑149 (MQ=255)
                                                   aGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGtt                                                                                         <  1:208460/149‑1 (MQ=255)
                                                                    gTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCAt                                                                        >  5:68850/1‑149 (MQ=255)
                                                                         cTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGtt                                                                    >  6:169895/1‑148 (MQ=255)
                                                                            gCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAg                                                                 <  8:87280/148‑1 (MQ=255)
                                                                             cGGATGATCTCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGc                                                                <  5:24429/148‑1 (MQ=255)
                                                                             cGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGca                                                               <  3:216955/149‑1 (MQ=255)
                                                                             cGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGc                                                                <  7:184188/148‑1 (MQ=255)
                                                                                      gCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATaa                                                                                     >  3:39924/1‑118 (MQ=255)
                                                                                      gCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATaa                                                                                     <  4:39924/118‑1 (MQ=255)
                                                                                       ccAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCgg                                                     >  4:195484/1‑149 (MQ=255)
                                                                                          gTAACCAGGCTTAATAACGGCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTc                                                  <  6:10472/149‑1 (MQ=255)
                                                                                               cAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCGCGACAATTTGCCACTGTTCGCCTCGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGGGGTGCGTTgg                                             >  1:110339/1‑149 (MQ=255)
                                                                                               cAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGtt                                                                    >  6:178857/1‑126 (MQ=255)
                                                                                               cAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTgg                                             >  1:248817/1‑149 (MQ=255)
                                                                                               cAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTgg                                             >  1:149949/1‑149 (MQ=255)
                                                                                                aGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGc                                            >  2:81038/1‑149 (MQ=255)
                                                                                                      aataaCGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCa                                                                         <  5:129505/114‑1 (MQ=255)
                                                                                                      aataaCGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCa                                                                         >  6:129505/1‑114 (MQ=255)
                                                                                                      aataaCGTCGATTTTCCTGCACCATATGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGGTATTAAGAATGTGGCGATCGTTATAAGAAACCCCGCCATTGATCAGCACAATGCGCGGTTCGTTGGCgggg                                        >  6:163324/1‑146 (MQ=255)
                                                                                                        taaCGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGc                                                                                                                                       >  2:119418/1‑50 (MQ=255)
                                                                                                        taaCGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGc                                                                                                                                       <  1:119418/50‑1 (MQ=255)
                                                                                                         taCGGCGATTTTCCGGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGc                                   <  6:72751/148‑1 (MQ=255)
                                                                                                         aaCGTCGATTTTCCTGCACCATTTGGCCCGAGATTTAGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTTTAAGAAACCACGCCATTTTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGc                                   <  5:163324/149‑1 (MQ=255)
                                                                                                         aaCGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGc                                   <  6:68850/149‑1 (MQ=255)
                                                                                                          aCGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGTCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCg                                  >  8:179290/1‑149 (MQ=255)
                                                                                                            gTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt                                 >  3:166200/1‑148 (MQ=255)
                                                                                                            gTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGt                                 >  1:211609/1‑148 (MQ=255)
                                                                                                             tCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCCGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCgaga                               >  3:178345/1‑149 (MQ=255)
                                                                                                                 ttttCCTGCACCATTTGGCACGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTATGAATGGGGCGATCGTTATAAGAAACCACGGCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTACGGCGTGACGTg                           <  1:218331/149‑1 (MQ=255)
                                                                                                                  tttCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGc                          >  3:45615/1‑149 (MQ=255)
                                                                                                                       tGCACCATTTGGCCCGACAATTTGCCAGTGATCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATCGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAg                     >  6:241342/1‑149 (MQ=255)
                                                                                                                         cACCATTTGGCCCGACAATTTGTCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAg                     <  7:179290/147‑1 (MQ=255)
                                                                                                                            cATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCa                >  2:141526/1‑149 (MQ=255)
                                                                                                                                   cccGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCAtccggct         <  2:211609/149‑1 (MQ=255)
                                                                                                                                    ccGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCAtccggctc        >  2:58476/1‑149 (MQ=255)
                                                                                                                                          aTTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAg  <  1:81038/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAG  >  NC_000913/792779‑793065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑