Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 830,034 T→C 7.7% N204D (AAC→GAC)  ybiH ← DUF1956 domain‑containing tetR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913830,0340TC 7.7% 152.8 / 3.5 52N204D (AAC→GAC) ybiHDUF1956 domain‑containing tetR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (25/23);  new base C (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

TTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCC  >  NC_000913/829891‑830180
                                                                                                                                               |                                                                                                                                                  
tttGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGAt                                                                                                                                                 >  7:121287/1‑147 (MQ=255)
    aTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGtt                                                                                                                                            <  3:210272/148‑1 (MQ=255)
        caccaGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTAGCGATAAGCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGCCCAGTTCGGTc                                                                                                                                       >  8:81460/1‑149 (MQ=255)
                gccgccGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGCCAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGGCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTttcttc                                                                                                                               <  2:199596/149‑1 (MQ=255)
                   tCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATc                                                                                                                            >  7:57242/1‑149 (MQ=255)
                   tCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATc                                                                                                                            >  7:5044/1‑149 (MQ=255)
                   tCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATc                                                                                                                            >  2:50401/1‑149 (MQ=255)
                            cACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGt                                                                                                                   >  1:21009/1‑149 (MQ=255)
                              cggcggCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTcc                                                                                                                 <  2:82761/149‑1 (MQ=255)
                                 cggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                                                                                              >  2:103110/1‑149 (MQ=255)
                                     aaGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGt                                                                                                          <  8:121287/149‑1 (MQ=255)
                                          ccACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCTAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGt                                                                                                      >  2:227576/1‑148 (MQ=255)
                                            ccTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTaa                                                                                                    >  5:164216/2‑148 (MQ=255)
                                                ccGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGaa                                                                                               >  8:47672/1‑149 (MQ=255)
                                                ccGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGa                                                                                                <  7:58553/148‑1 (MQ=255)
                                                 cGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAAt                                                                                              >  8:14390/1‑149 (MQ=255)
                                                 cGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAAt                                                                                              >  5:42646/1‑149 (MQ=255)
                                                  gCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAAtt                                                                                             <  7:14390/149‑1 (MQ=255)
                                                      aTCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGttt                                                                                         >  6:167983/1‑149 (MQ=255)
                                                       tCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTc                                                                                        <  6:102508/149‑1 (MQ=255)
                                                         cGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCtt                                                                                      >  5:104268/1‑149 (MQ=255)
                                                          gATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCttt                                                                                     <  6:42646/149‑1 (MQ=255)
                                                                gACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGcc                                                                                  >  6:187011/1‑146 (MQ=255)
                                                                 aCAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGa                                                                              <  5:167983/149‑1 (MQ=255)
                                                                      ttttttCATCTCTACAAACTCCTTTGCGTTATTCATTGCATAATCAGGTCGATATGACAGGTGACCGTCTGGTTGATGAGTTGGGTCTTTTCTTAATCGAACGCGGTCCAGCCGGTACGTTTCAGTATTGTTTGTTTGGCAAGACGGaa                                                                         <  8:34363/149‑1 (MQ=255)
                                                                      ttttttCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGaa                                                                         >  4:110616/1‑149 (MQ=255)
                                                                             atcaCTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGGCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGa                                                                  <  2:97534/149‑1 (MQ=255)
                                                                                aCTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGCTCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAAtc                                                               >  3:54212/1‑149 (MQ=255)
                                                                                  tACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAAt                                                                                              >  3:98182/1‑116 (MQ=255)
                                                                                  tACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAAt                                                                                              <  4:98182/116‑1 (MQ=255)
                                                                                  tACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTCGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAAc                                                                                                   <  7:25662/111‑1 (MQ=255)
                                                                                  tACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTCGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAAc                                                                                                   >  6:148789/1‑111 (MQ=255)
                                                                                  tACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTCGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAAc                                                                                                   >  8:25662/1‑111 (MQ=255)
                                                                                  tACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTCGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAAc                                                                                                   <  5:148789/111‑1 (MQ=255)
                                                                                     aaaCTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCAcc                                                          <  2:41294/149‑1 (MQ=255)
                                                                                      aaCTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACCCCAGAATCTCAcc                                                          >  3:172304/1‑148 (MQ=255)
                                                                                                 gATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATg                                              >  2:15941/1‑149 (MQ=255)
                                                                                                  aTAATCCTTGCAAAATCAGGTCGATATGACAGGGCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATgg                                             <  6:164216/149‑1 (MQ=255)
                                                                                                      tCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCttt                                                                                     <  6:139707/105‑1 (MQ=255)
                                                                                                      tCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCttt                                                                                     >  5:139707/1‑105 (MQ=255)
                                                                                                          tGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATAAGTTCGGTCTTTTCTTCATCGAACGCCGTCCAGCAGGAACGTAACAGAATACTTTCATTTCCAAGACGGAACTCCAGAATCTCACCCTAAAAAGCATGGGTATGAAg                                     >  6:19253/1‑149 (MQ=255)
                                                                                                           gCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGcc                                                                                  >  7:108410/1‑103 (MQ=255)
                                                                                                           gCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGcc                                                                                  <  8:108410/103‑1 (MQ=255)
                                                                                                            cAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGa                                   <  1:50401/149‑1 (MQ=255)
                                                                                                             aaaaTCAGGTCGATATGACAGGTCACCGTCTGGTTGACCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGAt                                  <  7:81460/149‑1 (MQ=255)
                                                                                                                    ggTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCgg                           <  1:15941/149‑1 (MQ=255)
                                                                                                                      tCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGgt                         <  1:227576/149‑1 (MQ=255)
                                                                                                                            tGACAGGTCACCGTCTGGTTGATGAGTTCGGGGTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAAGAGAATTGTTTCTTTGCCAAGACTGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGTGTGTCAt                    <  8:57242/148‑1 (MQ=255)
                                                                                                                                  gTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTc             >  7:92222/1‑149 (MQ=255)
                                                                                                                                  gTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTc             >  1:34797/1‑149 (MQ=255)
                                                                                                                                       cGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGc        <  1:27175/149‑1 (MQ=255)
                                                                                                                                             gTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTcc  <  1:160740/149‑1 (MQ=255)
                                                                                                                                              ttGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTg                  <  6:142967/132‑1 (MQ=255)
                                                                                                                                              ttGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTg                  >  5:142967/1‑132 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCC  >  NC_000913/829891‑830180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑