Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,005,777	A→G	5.5%	*337* (TAA→TGA)	pyrD →	dihydro‑orotate oxidase, FMN‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,005,777	0	A	G	5.5%	225.5 / 2.4	73	*337* (TAA→TGA)	pyrD	dihydro‑orotate oxidase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base A (35/34);  new base G (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

GTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGC  >  NC_000913/1005630‑1005922                                                                                                                                                    |                                                                                                                                                  gTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTaa                                                                                                                                                  <  7:193246/149‑1 (MQ=255)  tCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCATCACTGGTGCAAAATTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAat                                                                                                                                                 >  2:114143/1‑149 (MQ=255)    gCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAatt                                                                                                                                                <  6:221348/148‑1 (MQ=255)        aCCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTAttt                                                                                                                                            <  8:100993/148‑1 (MQ=255)        aCCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCACACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTAttt                                                                                                                                            <  5:49022/148‑1 (MQ=255)          cGATCATCGGTGTTGGCGGAATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATttctt                                                                                                                                         >  4:131346/1‑149 (MQ=255)           gttcatcGGTGTGGGCGTCATCGTCTCTGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTTGTTAAAATTTATTCTGGTTTTATTTTTAAAGTTCCGCCGCTGATTATATATATCGTTACCGATATCTAATTATttcttc                                                                                                                                        <  8:116307/147‑1 (MQ=255)                tcGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAAtt                                                                                                                                   >  2:191437/1‑149 (MQ=255)                   gtgtTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCct                                                                                                                                >  8:4733/1‑149 (MQ=255)                         gcggcATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTta                                                                                                                           >  8:167715/1‑148 (MQ=255)                            gcATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTtata                                                                                                                         <  6:160581/147‑1 (MQ=255)                            gcATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTtata                                                                                                                         >  5:160581/1‑147 (MQ=255)                              aTCGTCTCGGTTATCTCTGCGCGTGAAAAGATTGCTGCGTGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCAGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGAt                                                                                                                     <  2:197546/149‑1 (MQ=255)                              aTCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCTGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTGATTATTTCTTCGAATTCCTCTTTATAAGa                                                                                                                      >  3:204657/1‑148 (MQ=255)                              aTCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTGATTATTTCTTCGAATTCCTCTTTATAAGa                                                                                                                      >  1:224440/1‑148 (MQ=255)                                 gACTCGGTTATCGCTGCTCGTGAAAAGAGTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTATTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAAt                                                                                                                  <  6:160296/149‑1 (MQ=255)                                 gACTCGGTTATCGCTGCGCGTGAAAAGATTGGTGCGGGTGCCTCACTGGTGCATATTTTTTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATaa                                                                                                                   <  2:69103/148‑1 (MQ=255)                                    tCGGTTATCGCTGCGCGTGAAAAGGTTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAg                                                                                                                       >  6:222763/1‑141 (MQ=255)                                    tCGGTTATCGCTGCGCGTGAAAAGGTTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAg                                                                                                                       <  5:222763/141‑1 (MQ=255)                                    tCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAg                                                                                                               >  8:33578/1‑149 (MQ=255)                                             gCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTAtt                                                                                                      >  8:179759/1‑149 (MQ=255)                                             gCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTAtt                                                                                                      <  7:167715/149‑1 (MQ=255)                                                 cgcgTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTAtttt                                                                                                    <  8:224362/147‑1 (MQ=255)                                                    gTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGcc                                                                                               >  6:79126/1‑149 (MQ=255)                                                      gAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCt                                                                                             >  2:93485/1‑149 (MQ=255)                                                            aTTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGgttgt                                                                                       <  3:200423/149‑1 (MQ=255)                                                               gctgcGGTTGCCTCACTGGTGCAAATTTATTCTTGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGttt                                                                                     <  4:111074/148‑1 (MQ=255)                                                               gctgcGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGttt                                                                                     <  7:132645/148‑1 (MQ=255)                                                                ctgcGGGGGCCTCACTGGTGCAAATTTATTCTGGGGTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGtttt                                                                                    <  3:18678/148‑1 (MQ=255)                                                                  gcGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTtata                                                                                                                         <  6:65121/109‑1 (MQ=255)                                                                  gcGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTtata                                                                                                                         >  5:65121/1‑109 (MQ=255)                                                                  gcGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTtata                                                                                 <  3:131346/149‑1 (MQ=255)                                                                   cGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTtata                                                                                 <  6:60806/148‑1 (MQ=255)                                                                   cGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTtata                                                                                 <  1:191437/148‑1 (MQ=255)                                                                      gTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCa                                                                             <  7:33578/149‑1 (MQ=255)                                                                          cccACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATGTTCAGCCCTGGTTGTTTTATATTCATCGc                                                                         >  2:38275/3‑149 (MQ=255)                                                                          cTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGc                                                                         >  5:169061/1‑149 (MQ=255)                                                                           tCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCt                                                                        >  5:168/1‑149 (MQ=255)                                                                               tGGAGCAAATATATTCTGGTTTTAATTTTAAAGGTCCGCCGCTGATTAAAGAAATAGTTACCAATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATAGCTGTTg                                                                    >  2:178365/1‑149 (MQ=255)                                                                                 gggCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTACTTATTTCTTCGAATTCCTCTTTATAAGATAATCCGGGCGTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTg                                                                    <  1:105100/145‑1 (MQ=255)                                                                                   gCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTa                                                                >  7:51358/1‑149 (MQ=255)                                                                                   gCAAATTTATTCTGGTTTTATTTTTAAAGGTCAGCCTCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGATTTATTTTCAGCCCTGGTTGTTTTCTATTCATCGCTGTTGCtt                                                                 <  3:159216/148‑1 (MQ=255)                                                                                    cAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTAt                                                               >  2:113656/1‑149 (MQ=255)                                                                                     aaaTTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTAtt                                                              <  1:38275/149‑1 (MQ=255)                                                                                      aaTTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTAttt                                                             <  3:162861/149‑1 (MQ=255)                                                                                        tttATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAg                                                           <  1:113656/149‑1 (MQ=255)                                                                                        tttATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACACATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTAtt                                                              >  2:4802/1‑146 (MQ=255)                                                                                               tGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACAtttt                                                    >  7:117324/1‑149 (MQ=255)                                                                                                 gTTTTATTTTTAAAGGTCCTCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACAtttt                                                    <  2:172741/147‑1 (MQ=255)                                                                                                  ttttATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTa                                                 <  7:179759/149‑1 (MQ=255)                                                                                                   tttATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTAACAATATCTAATTATTTCTTCGAATTCCTATTTATAAGATAATCAGGGATTTATTTTCAGACCTGGTTGTTTTATATTCATAGATTTTGCTTATTTAGACATTATGTAc                                                >  5:145471/1‑149 (MQ=255)                                                                                                          ttAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTAtt                                         >  4:105440/1‑149 (MQ=255)                                                                                                             aaGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTAttgtt                                      <  5:79126/149‑1 (MQ=255)                                                                                                                gTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTGTTATTGTTGAg                                   >  8:115042/1‑149 (MQ=255)                                                                                                                     ccgcTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGttat                              >  8:241386/1‑149 (MQ=255)                                                                                                                       gcTGATTAAAGAAATCGTTTCCCATATCTAATTATTTCTTCTAATTCATCTTTTTAAGATTATGCGGTCTTTATTTTGAGCCCTGGTTGTATTATATTCATCGCTGTTGCTTATTTAGACATTATTTACTTTTATTTTTGATGttatta                            <  4:229577/149‑1 (MQ=255)                                                                                                                       gcTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCt                                                                        <  4:54128/105‑1 (MQ=255)                                                                                                                       gcTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCt                                                                        >  3:54128/1‑105 (MQ=255)                                                                                                                         tGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTAttg                                        <  1:200155/135‑1 (MQ=255)                                                                                                                         tGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTAttg                                        >  2:200155/1‑135 (MQ=255)                                                                                                                               aaGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTaagcgaagc                    >  1:48847/1‑149 (MQ=255)                                                                                                                                  aaaTCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGAc                 <  7:241386/149‑1 (MQ=255)                                                                                                                                       gTTACCCATATCTGATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGTCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATg             <  4:204657/148‑1 (MQ=255)                                                                                                                                       gTTACCCATATCTGATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGTCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATg             <  2:224440/148‑1 (MQ=255)                                                                                                                                        ttACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGa           >  7:157205/1‑149 (MQ=255)                                                                                                                                            ccATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATtgt       >  4:145255/1‑149 (MQ=255)                                                                                                                                            ccATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATtgt       >  2:1076/1‑149 (MQ=255)                                                                                                                                            ccATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATtgt       >  3:122203/1‑149 (MQ=255)                                                                                                                                               tatCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGAc                 <  5:196128/136‑1 (MQ=255)                                                                                                                                               tatCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGAc                 >  6:196128/1‑136 (MQ=255)                                                                                                                                               tatCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATCAAGCGAAGCGACAATGGATTGTGtt    >  1:63983/1‑149 (MQ=255)                                                                                                                                                 tCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGtta                               >  1:7737/1‑120 (MQ=255)                                                                                                                                                 tCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGtta                               <  2:7737/120‑1 (MQ=255)                                                                                                                                                 tCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgc  >  5:55543/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCGGTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGC  >  NC_000913/1005630‑1005922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑