Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,137,271 | G→A | 6.9% | R333H (CGC→CAC) | flgI → | putative flagellar basal body protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,137,271 | 0 | G | A | 6.9% | 178.7 / 2.7 | 58 | R333H (CGC→CAC) | flgI | putative flagellar basal body protein |
Reads supporting (aligned to +/- strand): ref base G (29/24); new base A (2/2); total (32/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 |
TTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTG > NC_000913/1137124‑1137408 | ttAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGAGAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTgcgc < 8:145485/149‑1 (MQ=255) gTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCt > 6:38114/1‑148 (MQ=255) ggcAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATgc > 6:106024/3‑149 (MQ=255) tCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGgc > 8:106127/1‑149 (MQ=255) gccagccaGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCACGCTCAATGCGCTGGGCGCt < 4:218065/149‑1 (MQ=255) ccagccagATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCCGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTa > 5:182983/1‑149 (MQ=255) agATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGa < 5:106024/149‑1 (MQ=255) gATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGAt > 8:104956/1‑149 (MQ=255) acCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATc > 6:91456/1‑149 (MQ=255) acCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCACGCGCTCAATGCGCTGGGCGCTACGCCGATGGATc > 3:4203/1‑149 (MQ=255) acCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGAATTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCACGCGCTCAATGCGCTGGGCGCTACGCCGATGGATc > 1:16127/1‑149 (MQ=255) ccGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCt < 4:132281/149‑1 (MQ=255) cGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTg < 5:38114/149‑1 (MQ=255) cGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTg < 7:104956/149‑1 (MQ=255) cGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTg < 7:4983/149‑1 (MQ=255) gTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGa < 5:44607/149‑1 (MQ=255) tggtggACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCa > 4:198416/1‑149 (MQ=255) gtggACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATa < 2:239525/149‑1 (MQ=255) gtggACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATa > 5:75884/1‑149 (MQ=255) gACAGACTGTGGTTACTCCACACACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTg > 6:44244/1‑149 (MQ=255) aCTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGcaat > 4:113175/1‑148 (MQ=255) aCTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGcaat > 4:189003/1‑148 (MQ=255) aCTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGcaat > 6:123463/1‑148 (MQ=255) tgGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCTTACCTTCCATCTCAAGAATCAATAAAGTGGTGATCGCGCTCAATGCTCTATGATCTACGCCGATGGATCTGATGTCCATACTGcaatcaat > 7:173658/1‑148 (MQ=255) tgGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGcaatcaa < 3:113175/147‑1 (MQ=255) tCCACAAACGCAGCTAGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCCGCGCCAGCCTCAATAACGTGGTGCGAGCGCTCAATGCGATGGGCGCTACGCCGCTGGATCAGCTGTCCATACTTCAATCAAGGCAAAGTg > 1:212686/1‑149 (MQ=255) cacaAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAACGCGTACGTTCCAGCGCCCGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCg > 3:183038/1‑149 (MQ=255) cacaAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCg > 8:119682/1‑149 (MQ=255) cacaAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCg > 1:112241/1‑149 (MQ=255) cacaAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCg > 4:64053/1‑149 (MQ=255) cacaAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACATGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCg > 4:92686/1‑149 (MQ=255) acaAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCgg < 2:130852/149‑1 (MQ=255) acaAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACATGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCgg < 3:92686/149‑1 (MQ=255) aGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTg < 3:67835/149‑1 (MQ=255) aTCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCg > 8:8563/1‑149 (MQ=255) aCGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCACGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCaaaa < 2:16127/148‑1 (MQ=255) aCGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCACGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCaaaa < 4:4203/148‑1 (MQ=255) gCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGc > 1:31686/1‑138 (MQ=255) gCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGc < 2:31686/138‑1 (MQ=255) cAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTgg > 6:44611/1‑148 (MQ=255) agagCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGa > 8:205904/1‑148 (MQ=255) gcggcgGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAatc < 5:123463/149‑1 (MQ=255) cggcggTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGGCTGCGGGCAAAACTGGAAatca < 3:64053/149‑1 (MQ=255) gcggTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAatcatc < 3:189003/149‑1 (MQ=255) ttCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCtgat < 3:198416/149‑1 (MQ=255) gCTGCAACGCGTACGTTCCAGCGCCAGCCTCAATCACGTGGTGCGCGCGCTCAATGCGCGGGGCGCTACGCCGATGGATCTGATGTCCTTAGTGCAATCAATGCAAAGTGCGGGATGGCTGCGGGCAAAACTGGAAATCATCTgatgat < 2:212686/149‑1 (MQ=255) cTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTgatgat > 3:167807/1‑148 (MQ=255) cTGCAAAGCGTACGGTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATc < 3:106406/149‑1 (MQ=255) aaaGCGTACGGTCCATCGCCAGCATCAATAACGTGGGGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACCGGAAATCATCTGATGATCAGCg < 4:183038/149‑1 (MQ=255) aaGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGa < 7:119682/149‑1 (MQ=255) gCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACa < 6:75884/149‑1 (MQ=255) cGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCaa < 5:44611/148‑1 (MQ=255) gTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAac > 8:123857/1‑149 (MQ=255) ttCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAac > 2:156377/1‑148 (MQ=255) tCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAacta > 4:18508/1‑149 (MQ=255) cAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAg > 5:122443/1‑149 (MQ=255) cTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCg > 4:91159/1‑149 (MQ=255) aTAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCt < 6:122443/149‑1 (MQ=255) tAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTg > 5:52023/1‑149 (MQ=255) | TTAATCGTCAGGCCAATGTCAGCCAGCCAGATACACCGTTTGGTGGTGGACAGACTGTGGTTACTCCACAAACGCAGATCGATTTACGCCAGAGCGGCGGTTCGCTGCAAAGCGTACGTTCCAGCGCCAGCCTCAATAACGTGGTGCGCGCGCTCAATGCGCTGGGCGCTACGCCGATGGATCTGATGTCCATACTGCAATCAATGCAAAGTGCGGGATGTCTGCGGGCAAAACTGGAAATCATCTGATGATCAGCGACAGCAAACTACTGGCAAGTGCGGCCTG > NC_000913/1137124‑1137408 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |