Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,224,478 T→C 5.4% Q23R (CAG→CGG)  minE ← cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,4780TC 5.4% 229.7 / 2.4 74Q23R (CAG→CGG) minEcell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base T (34/36);  new base C (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

TATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGTTCTTCT  >  NC_000913/1224332‑1224618
                                                                                                                                                  |                                                                                                                                            
tatCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTg                                                                                                                                             >  6:4365/1‑148 (MQ=255)
  tCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAg                                                                                                                                          <  4:62188/149‑1 (MQ=255)
      cATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGt                                                                                                                                      >  6:180900/1‑149 (MQ=255)
            ttttatCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTc                                                                                                                                    <  2:196668/140‑1 (MQ=255)
              tGCTCAAGCTGTACGTTTAACATCTCAGGATCAATTTGTACTTATTTACAAATGACCTCAAGATTATCTTTACGCATCTGCGGCAGATATTGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTg                                                                                                                               <  3:171024/148‑1 (MQ=255)
              tGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTg                                                                                                                               <  8:183223/148‑1 (MQ=255)
                cTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCaa                                                                                                                            >  2:215761/1‑149 (MQ=255)
                 tCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAAt                                                                                                                           >  2:2029/1‑149 (MQ=255)
                   aaGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGt                                                                                                                         >  3:194589/1‑149 (MQ=255)
                   aaGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGt                                                                                                                         >  1:41536/1‑149 (MQ=255)
                   aaGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGt                                                                                                                         >  7:74473/1‑149 (MQ=255)
                     gCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGtt                                                                                                                        <  8:63489/148‑1 (MQ=255)
                     gCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGtt                                                                                                                        >  7:63489/1‑148 (MQ=255)
                      cTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTgg                                                                                                                      <  7:60736/149‑1 (MQ=255)
                            ggTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTgtgt                                                                                                                >  2:202022/1‑149 (MQ=255)
                                 ccATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTttc                                                                                                           <  6:7874/149‑1 (MQ=255)
                                 ccATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTttc                                                                                                           <  7:171205/149‑1 (MQ=255)
                                  cctctcAGGATCAAGTTGTACGGATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGGTCTGCATCGCTGCGACGGCGTGCAGCAACAATAAGCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGGTTtgct                                                                                                          <  1:52549/147‑1 (MQ=255)
                                    tctcAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTttcttc                                                                                                        >  3:116605/1‑149 (MQ=255)
                                       cAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGAAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTCCAGCCGTTCTTATGCAATGTTGGCTGTGTTTTTCTTCagc                                                                                                     >  4:113297/1‑149 (MQ=255)
                                        aGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCcgc                                                                                                     <  1:202022/148‑1 (MQ=255)
                                        aGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCcgc                                                                                                     <  7:96333/148‑1 (MQ=255)
                                           aTCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCgaga                                                                                                 <  5:4365/149‑1 (MQ=255)
                                               ctttGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCgagaga                                                                                               >  7:175928/2‑147 (MQ=255)
                                               aTTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCgagaga                                                                                               >  3:86029/1‑147 (MQ=255)
                                                tttGTAGGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCTGAATATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCgaga                                                                                                 <  5:217346/144‑1 (MQ=255)
                                                    tACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAAt                                                                                        <  2:41536/149‑1 (MQ=255)
                                                           ttACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGCCGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTaa                                                                                 >  6:172335/1‑149 (MQ=255)
                                                                      cTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGAGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAAc                                                                        <  6:231712/147‑1 (MQ=255)
                                                                      cTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGAGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAAc                                                                        >  5:231712/1‑147 (MQ=255)
                                                                        cAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCCGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTAt                                                                    >  2:130720/1‑149 (MQ=255)
                                                                             atatCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGCTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTAtcctcc                                                               <  6:33258/149‑1 (MQ=255)
                                                                                tCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGa                                                             <  5:132935/148‑1 (MQ=255)
                                                                                tCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCATCCGaa                                                            >  1:74554/1‑149 (MQ=255)
                                                                                 cTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGaa                                                            >  8:229963/1‑148 (MQ=255)
                                                                                  tttACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGCCGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGacc                                                           >  2:194178/1‑146 (MQ=255)
                                                                                    tACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAg                                                        >  3:76831/1‑149 (MQ=255)
                                                                                           cTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGa                                                 <  7:229963/149‑1 (MQ=255)
                                                                                              cGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAg                                                <  5:95707/147‑1 (MQ=255)
                                                                                                gCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGc                                                                              <  2:79544/115‑1 (MQ=255)
                                                                                                gCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGc                                                                              >  1:79544/1‑115 (MQ=255)
                                                                                                gCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAg                                            >  4:92759/1‑149 (MQ=255)
                                                                                                    aTAAAGCGGTTCTGCATCGCTGCGCCGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAAACTTGGCTGTGTTTTTCTTCCCCGAGAGAAAGAAATCGAGTAATGCCATAACTTAACCTCCGCACAACCCTTTCAGGAAGCCtt                                        >  8:112243/1‑149 (MQ=255)
                                                                                                     tAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCttt                                       <  5:180900/149‑1 (MQ=255)
                                                                                                      aaTGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCCGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTttc                                      >  4:85201/1‑149 (MQ=255)
                                                                                                          cGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATTATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCGGCGAGAGAAAGAAATCGAGTAATTCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTtcttct                                  <  7:112243/149‑1 (MQ=255)
                                                                                                             ttCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTtcttctctt                               <  8:74473/149‑1 (MQ=255)
                                                                                                             ttCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTtcttctctt                               <  4:86029/149‑1 (MQ=255)
                                                                                                             ttCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTtcttctct                                >  4:196708/1‑148 (MQ=255)
                                                                                                             ttCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCCGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTtcttctctt                               <  3:85201/149‑1 (MQ=255)
                                                                                                                 gCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGt                                                     >  2:21603/1‑123 (MQ=255)
                                                                                                                 gCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGt                                                     >  4:73887/1‑123 (MQ=255)
                                                                                                                 gCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGt                                                     <  3:73887/123‑1 (MQ=255)
                                                                                                                 gCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGt                                                     <  1:21603/123‑1 (MQ=255)
                                                                                                                 gCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTcttctt                            >  1:147374/1‑148 (MQ=255)
                                                                                                                  cATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTcttctt                            <  1:215761/147‑1 (MQ=255)
                                                                                                                   atggCTGCGACGGCGTTCAGCAACAATAATCTGCAGGCGTTATTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTTATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCaa                         <  3:113297/146‑1 (MQ=255)
                                                                                                                   aTCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCaa                         >  6:191134/1‑149 (MQ=255)
                                                                                                                    tCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAAt                        <  4:76831/149‑1 (MQ=255)
                                                                                                                    tCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAAt                        <  3:232827/149‑1 (MQ=255)
                                                                                                                     cGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAAt                        >  7:57692/1‑148 (MQ=255)
                                                                                                                        tGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAAt                                                                                <  8:155439/89‑1 (MQ=255)
                                                                                                                        tGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAAt                                                                                >  7:155439/1‑89 (MQ=255)
                                                                                                                              ggCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGaaa              <  8:57692/149‑1 (MQ=255)
                                                                                                                               gCGTTCAGCAACAATAATCCGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAg             <  1:130720/149‑1 (MQ=255)
                                                                                                                                 gTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGa           <  3:92759/149‑1 (MQ=255)
                                                                                                                                    cAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACg         <  5:61963/148‑1 (MQ=255)
                                                                                                                                     aGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCAGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGtt       >  4:186426/1‑149 (MQ=255)
                                                                                                                                     aGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCAGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGAGGAAAGGACGt        >  4:90777/1‑148 (MQ=255)
                                                                                                                                      gCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGa                                                 >  5:44249/1‑106 (MQ=255)
                                                                                                                                      gCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGa                                                 <  6:44249/106‑1 (MQ=255)
                                                                                                                                        aacaaTAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGttctt    >  5:191551/1‑149 (MQ=255)
                                                                                                                                         acaaTAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGttcttc   >  7:130351/1‑149 (MQ=255)
                                                                                                                                          caaTAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGTtcttct  <  3:196708/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTTCTTTTGCAATGTTGGCTGTGTTTTTCTTCCGCGAGAGAAAGAAATCGAGTAATGCCATAACTTATCCTCCGAACAAGCGTTTGAGGAAGCCTTTCTTCTCTTCTTCAATGAAGCGGAAAGGACGTTCTTCT  >  NC_000913/1224332‑1224618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: