Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,230,550 A→G 6.1% intergenic (‑156/‑217) hlyE ← / → umuD hemolysin E/translesion error‑prone DNA polymerase V subunit; RecA‑activated auto‑protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,230,5500AG 6.1% 196.1 / 2.9 65intergenic (‑156/‑217)hlyE/umuDhemolysin E/translesion error‑prone DNA polymerase V subunit; RecA‑activated auto‑protease
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base G (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GCCTCTTTAAATATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCT  >  NC_000913/1230403‑1230695
                                                                                                                                                   |                                                                                                                                                 
gCCTCTTTAAATATATAAATTGTACTGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATCAATATGATAAATATCAAACAATGTTTAATGTCa                                                                                                                                                   <  3:160149/148‑1 (MQ=255)
 ccTCTTTAAATATATAAATTGTAATGAAACTCGTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCAtt                                                                                                                                                 <  5:93495/149‑1 (MQ=255)
     tttAAATATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTAt                                                                                                                                               <  2:85767/147‑1 (MQ=255)
        aaaTATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCg                                                                                                                                           >  7:88517/1‑148 (MQ=255)
         aaTATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGaa                                                                                                                                         <  4:157044/149‑1 (MQ=255)
         aaTATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGGCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGaa                                                                                                                                         <  5:173121/149‑1 (MQ=255)
              ataAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCGTTATGGCGAATGCt                                                                                                                                     >  6:66567/1‑148 (MQ=255)
               taAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCtt                                                                                                                                    <  8:152754/148‑1 (MQ=255)
                 aaTTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCACTGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCttcta                                                                                                                                 <  5:189487/149‑1 (MQ=255)
                   ttGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCttctatt                                                                                                                               >  4:142323/1‑149 (MQ=255)
                      tAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCttctattcta                                                                                                                            >  8:145771/1‑149 (MQ=255)
                      tAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCttct                                                                                                                                  <  6:154432/143‑1 (MQ=255)
                      tAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCttct                                                                                                                                  >  5:154432/1‑143 (MQ=255)
                        aTGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTtctattctat                                                                                                                           <  7:82328/148‑1 (MQ=255)
                          gAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTAtttt                                                                                                                        >  6:143779/1‑149 (MQ=255)
                             aCTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCGTTATGGCGAATGCTTCTATTCTATTTTTTa                                                                                                                     <  5:66567/149‑1 (MQ=255)
                             aCTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTa                                                                                                                     <  3:218800/149‑1 (MQ=255)
                                 cTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGcc                                                                                                                  <  7:244350/148‑1 (MQ=255)
                                         cAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGatat                                                                                                         >  6:214794/1‑149 (MQ=255)
                                               ttAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCa                                                                                                   <  7:145771/149‑1 (MQ=255)
                                                   taaATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTc                                                                                               >  3:64763/1‑149 (MQ=255)
                                                   taaATTTTACTTCATATAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTGTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTc                                                                                               >  3:193284/1‑149 (MQ=255)
                                                       ttttACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTctgct                                                                                           <  2:15404/149‑1 (MQ=255)
                                                             ttCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCGTTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGAt                                                                                       >  6:168448/1‑147 (MQ=255)
                                                                 tCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATGAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGagt                                                                                 >  5:63281/1‑149 (MQ=255)
                                                                    aaTTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGcgtcgt                                                                              >  8:51926/1‑149 (MQ=255)
                                                                     aTTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGACTGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGcgtc                                                                                <  5:70223/146‑1 (MQ=255)
                                                                         aTAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGATTCTATTCTATTTTTTATCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGc                                                                           >  2:151976/1‑147 (MQ=255)
                                                                            gTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAg                                                                        >  8:186109/1‑147 (MQ=255)
                                                                             ttAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGt                                                                                                              <  1:132611/108‑1 (MQ=255)
                                                                             ttAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGt                                                                                                              >  2:132611/1‑108 (MQ=255)
                                                                                gCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGcc                                                                  <  4:64763/149‑1 (MQ=255)
                                                                                  cgggcgggATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCAc                                                                <  7:34269/149‑1 (MQ=255)
                                                                                         gATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGca                                                         >  7:92262/1‑149 (MQ=255)
                                                                                             cGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACaa                                                      <  4:193284/148‑1 (MQ=255)
                                                                                              gTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGa                                                                                     >  2:223320/1‑116 (MQ=255)
                                                                                              gTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGa                                                                                     <  1:223320/116‑1 (MQ=255)
                                                                                                 aaTGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATaa                                                 <  5:214794/149‑1 (MQ=255)
                                                                                                  aTGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGAt                                                   >  4:177565/1‑146 (MQ=255)
                                                                                                  aTGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGAt                                                   >  1:242311/1‑146 (MQ=255)
                                                                                                  aTGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAag                                                <  7:53729/149‑1 (MQ=255)
                                                                                                   tGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTATGCTGGATGAGCGTCGTCGCCAGAAGTCCACGTGAGCACAAGAt                                                   >  1:224687/1‑145 (MQ=255)
                                                                                                    ttCTTTATTTCTATTAATATTATAAATATCAAACAATTTTTAATGTCATTATGTCTAATTCTTATATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAaga                                               <  8:174481/147‑1 (MQ=255)
                                                                                                    gTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGTAGGCCACGTGAGCACaa                                                      >  7:231028/1‑141 (MQ=255)
                                                                                                    gTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACaa                                                      <  8:231028/141‑1 (MQ=255)
                                                                                                      cTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTAATTATGTATAGTTCTTCTATTCTATTTTTTAGCAGGGTGATATTTATAATTTATCATTGATGATCGTCTTCGCCAGAAGGCAAAGTGATCACAAGATAaga                                               >  7:174481/1‑146 (MQ=255)
                                                                                                       tttATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATaa                                                 <  2:38015/143‑1 (MQ=255)
                                                                                                       tttATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATaa                                                 >  1:38015/1‑143 (MQ=255)
                                                                                                       tttATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAAc                                           >  3:152533/1‑149 (MQ=255)
                                                                                                         tATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCGTTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAAc                                           <  5:168448/147‑1 (MQ=255)
                                                                                                          aTTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCGTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGa                                         <  6:135456/148‑1 (MQ=255)
                                                                                                                 ttAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAAt                                    <  7:186109/146‑1 (MQ=255)
                                                                                                                 ttAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATcag                                 >  4:196491/1‑149 (MQ=255)
                                                                                                                   aaTATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATcagc                                <  2:242311/148‑1 (MQ=255)
                                                                                                                    atatGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATcagcag                              >  6:180254/1‑149 (MQ=255)
                                                                                                                             atatCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAg                     <  3:177565/149‑1 (MQ=255)
                                                                                                                               atCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCg                   >  5:42308/1‑149 (MQ=255)
                                                                                                                                   aaCAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACa                >  2:57606/1‑148 (MQ=255)
                                                                                                                                   aaCAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACa                >  6:15348/1‑148 (MQ=255)
                                                                                                                                    aCAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGc                                                                                   >  3:187145/1‑80 (MQ=255)
                                                                                                                                    aCAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGc                                                                                   <  4:187145/80‑1 (MQ=255)
                                                                                                                                      aaTGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAata            >  1:75006/1‑149 (MQ=255)
                                                                                                                                        tGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATAtt          <  8:88517/149‑1 (MQ=255)
                                                                                                                                              aTGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTTATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATAtt          <  7:113159/143‑1 (MQ=255)
                                                                                                                                              aTGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGTATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATAtt          >  8:113159/1‑143 (MQ=255)
                                                                                                                                                 tCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCt  <  3:196491/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCCTCTTTAAATATATAAATTGTAATGAAACTCCTGTTTTACAACTATTAATAAATTTTACTTCATCTAATTCATAGTTAGCCGGGCGGGATGCGTCAATGTCTTTATTTCTATTAATATGATAAATATCAAACAATGTTTAATGTCATTATGGCGAATGCTTCTATTCTATTTTTTAGCCGGGTGATATTTTTCATTTCTGCTGGATGAGCGTCGTCGCCAGAAGGCCACGTGAGCACAAGATAAGAGAACGAAAAATCAGCAGCCTATGCAGCGACAAATATTGATAGCCT  >  NC_000913/1230403‑1230695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: