Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,239,380 G→A 7.8% G168R (GGA→AGA)  dadX → alanine racemase, catabolic, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,239,3800GA 7.8% 150.7 / 3.0 51G168R (GGA→AGA) dadXalanine racemase, catabolic, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base A (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

GGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTC  >  NC_000913/1239232‑1239525
                                                                                                                                                    |                                                                                                                                                 
ggATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATg                                                                                                                                                   >  4:31112/1‑149 (MQ=255)
        aTCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTc                                                                                                                                            <  4:217984/148‑1 (MQ=255)
         tCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCg                                                                                                                                          >  5:74458/1‑149 (MQ=255)
                gTGAACAGTGGGATGACTCGGTTGGGCTGCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGAt                                                                                                                                   <  5:3911/149‑1 (MQ=255)
                     cAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGgcgc                                                                                                                              >  5:206367/1‑149 (MQ=255)
                         gggATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc                                                                                                                                        <  6:235140/135‑1 (MQ=255)
                         gggATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGgc                                                                                                                                        >  5:235140/1‑135 (MQ=255)
                          ggATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTAtt                                                                                                                         <  6:38107/149‑1 (MQ=255)
                          ggATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTAACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTAGCATTTTGCCGAAGCGGAACGTCCTGATGGAATTTCCGGCGCGATGGCGCGTAtt                                                                                                                         >  2:51933/1‑149 (MQ=255)
                            aggAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGa                                                                                                                       <  6:64859/147‑1 (MQ=255)
                            aTGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGa                                                                                                                       <  7:168229/149‑1 (MQ=255)
                            aTGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGa                                                                                                                       <  3:31112/149‑1 (MQ=255)
                                 tCGGTTGGGCTTCCAGCCCGATCGCGTGCTAACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAgg                                                                                                                  >  2:87233/1‑149 (MQ=255)
                                   ggTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAggcg                                                                                                                >  6:106057/1‑149 (MQ=255)
                                    gTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAggcgg                                                                                                               >  7:170878/1‑149 (MQ=255)
                                        ggCTTCCAGCTCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGa                                                                                                           <  5:48231/149‑1 (MQ=255)
                                               aGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGCATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTg                                                                                                    >  3:181783/1‑149 (MQ=255)
                                               aGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTg                                                                                                    <  4:197939/149‑1 (MQ=255)
                                                 cccGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGa                                                                                                  <  2:69016/149‑1 (MQ=255)
                                                             cTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATATCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGcgttc                                                                                      >  6:86244/1‑149 (MQ=255)
                                                              ttACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGcgttcg                                                                                     >  7:168977/1‑149 (MQ=255)
                                                                aCCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATACTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGcgttcgtt                                                                                   >  3:214053/1‑149 (MQ=255)
                                                                 ccGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACGTCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGcgttcgtt                                                                                   <  1:51933/148‑1 (MQ=255)
                                                                 ccGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGcgttcgtt                                                                                   <  4:181783/148‑1 (MQ=255)
                                                                  cGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTTTTGAGCAGGCGGCGGAGGGGGTGGAGTGTCGGCGTTCGTTGt                                                                                 <  6:206367/149‑1 (MQ=255)
                                                                   gTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTc                                                                                <  8:178899/149‑1 (MQ=255)
                                                                        gcagcaGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAAt                                                                            <  1:87233/148‑1 (MQ=255)
                                                                        gcagcaGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATAGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAAtt                                                                           >  6:41801/1‑149 (MQ=255)
                                                                        gcagcaGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATAGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAAtt                                                                           >  6:45271/1‑149 (MQ=255)
                                                                               ttGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCggcggc                                                                    <  5:106057/149‑1 (MQ=255)
                                                                                       aaTGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCtgt                                                            <  8:168977/149‑1 (MQ=255)
                                                                                       aaTGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCtgt                                                            >  7:195191/1‑149 (MQ=255)
                                                                                           gCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCa                                                        >  4:88373/1‑149 (MQ=255)
                                                                                                  ttGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGaa                                                 <  5:86244/149‑1 (MQ=255)
                                                                                                     gCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATAGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAgc                                               <  5:45271/148‑1 (MQ=255)
                                                                                                     gCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATAGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAgc                                               <  5:41801/148‑1 (MQ=255)
                                                                                                             aCCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGa                                      >  1:135697/1‑149 (MQ=255)
                                                                                                              cccTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCTGCGGCGACTCtgtg                                                           >  6:95807/1‑127 (MQ=255)
                                                                                                               ccTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACt                                    >  2:153419/1‑149 (MQ=255)
                                                                                                               ccTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACt                                    <  6:74458/149‑1 (MQ=255)
                                                                                                                cTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTg                                   >  4:95116/1‑149 (MQ=255)
                                                                                                                        gCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGc                           <  8:195191/149‑1 (MQ=255)
                                                                                                                              tGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTTTGTCACCAGGAAGCGCATTTTGACTGGGTTCGGCCTGGc                      <  2:143798/148‑1 (MQ=255)
                                                                                                                                ccGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCt                         <  2:43057/143‑1 (MQ=255)
                                                                                                                                ccGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCt                         >  1:43057/1‑143 (MQ=255)
                                                                                                                                           cATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGAGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATgg        >  8:121928/1‑149 (MQ=255)
                                                                                                                                               cTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCCTTTTGACTGGGTTCGGCCGGGAATTATTTTGTATGGCGCt    >  6:216073/1‑149 (MQ=255)
                                                                                                                                               atGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGGGGCACCCGGAAGGGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCt    >  7:174922/2‑149 (MQ=255)
                                                                                                                                                tGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGTAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCt    >  3:34170/1‑148 (MQ=255)
                                                                                                                                                tGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCtt   >  5:209328/1‑149 (MQ=255)
                                                                                                                                                  aTGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTc  <  4:214053/148‑1 (MQ=255)
                                                                                                                                                  aTGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTc  <  1:8384/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCCAGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTTGCCGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCGTTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTC  >  NC_000913/1239232‑1239525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑