Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,939 A→G 8.9% I229T (ATC→ACC)  purU ← formyltetrahydrofolate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,9390AG 8.9% 126.8 / 3.2 45I229T (ATC→ACC) purUformyltetrahydrofolate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (18/23);  new base G (2/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGC  >  NC_000913/1287792‑1288086
                                                                                                                                                   |                                                                                                                                                   
atCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCAtgatga                                                                                                                                                     <  8:157524/148‑1 (MQ=255)
 tCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGAtt                                                                                                                                                   <  5:70961/149‑1 (MQ=255)
            ccGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGt                                                                                                                                         <  1:230083/148‑1 (MQ=255)
             cGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACAGGAATAACGTCCTGCATGATGATTGGGCCTTCGtg                                                                                                                                        >  3:198849/1‑147 (MQ=255)
              gTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTc                                                                                                                                        >  3:21398/1‑147 (MQ=255)
                    aaaGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGGTTGGGCCTTCGTc                                                                                                                                        >  3:163776/1‑141 (MQ=255)
                    aaaGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGGTTGGGCCTTCGTc                                                                                                                                        >  3:166837/1‑141 (MQ=255)
                    aaaGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGGTTGGGCCTTCGTc                                                                                                                                        <  4:166837/141‑1 (MQ=255)
                    aaaGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGGTTGGGCCTTCGTc                                                                                                                                        <  4:163776/141‑1 (MQ=255)
                    aaaGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGt                                                                                                                                >  4:87332/1‑149 (MQ=255)
                     aaGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTc                                                                                                                               <  3:87332/149‑1 (MQ=255)
                          gcgcTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTca                                                                                                                          >  5:159853/1‑149 (MQ=255)
                              tgtgCCAGTACTTAGTATAGTGCACTACTATAGACTTTTTTCTCGACGTCATGACCTGCGCGCATCATATCTTCTGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTcaca                                                                                                                        <  8:216841/147‑1 (MQ=255)
                                tgCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGt                                                                                                                    <  8:24296/149‑1 (MQ=255)
                                        cTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGAAGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGtt                                                                                                            <  4:167942/149‑1 (MQ=255)
                                               taGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCaa                                                                                                     >  2:230138/1‑149 (MQ=255)
                                                        aCTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTca                                                                                            <  7:61121/149‑1 (MQ=255)
                                                             aGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCa                                                                                                                                      >  3:167250/1‑102 (MQ=255)
                                                             aGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCa                                                                                                                                      <  4:167250/102‑1 (MQ=255)
                                                              gACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCAcaccac                                                                                      >  6:12919/1‑149 (MQ=255)
                                                                 gTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGtt                                                                                   <  8:103977/149‑1 (MQ=255)
                                                                 gTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGtt                                                                                   <  8:218966/149‑1 (MQ=255)
                                                                          gACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAtt                                                                                                                                  >  3:215332/1‑93 (MQ=255)
                                                                          gACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAtt                                                                                                                                  <  4:215332/93‑1 (MQ=255)
                                                                          gACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAgg                                                                             <  5:12919/146‑1 (MQ=255)
                                                                           ccGTCACGAGCTGGGCGCATCATATCTTCAGCTGTGTAGGTATCACCGACATGAATAAGGGCCTGCATGATGATTGGGACTTCGTCCAGATTGTCATTCACTTAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTg                                                                         <  6:29331/148‑1 (MQ=255)
                                                                             gTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGt                                                                       <  8:71158/149‑1 (MQ=255)
                                                                                  gACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATaa                                                                  <  4:21398/149‑1 (MQ=255)
                                                                                             atcatATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTgcgc                                                        >  1:138081/1‑148 (MQ=255)
                                                                                                             tgtAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCa                                         <  7:18332/147‑1 (MQ=255)
                                                                                                             tgtAGGTATGATCGACATGAAAAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCAAAAAGTGAGCGGTTGCGCCAAAAATCTTCAAACCACGATCATAGGCCAGGTGATAAGGAAGTGCGCCAAAAAACgcaggcag                                        >  6:27049/1‑148 (MQ=255)
                                                                                                                 ggTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGgaat                                    >  7:124910/1‑148 (MQ=255)
                                                                                                                    aTGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGAc                                                              <  5:119492/119‑1 (MQ=255)
                                                                                                                    aTGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGAc                                                              >  6:119492/1‑119 (MQ=255)
                                                                                                                     tGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTg                                                           >  3:183392/1‑121 (MQ=255)
                                                                                                                     tGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTg                                                           <  4:183392/121‑1 (MQ=255)
                                                                                                                      gATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTcac                                                                                           >  7:153446/1‑88 (MQ=255)
                                                                                                                      gATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTcac                                                                                           <  8:153446/88‑1 (MQ=255)
                                                                                                                          gACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGaa                          <  1:230138/149‑1 (MQ=255)
                                                                                                                           aCATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGaa                          >  8:249176/1‑148 (MQ=255)
                                                                                                                             aTGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAatat                       >  4:204916/1‑149 (MQ=255)
                                                                                                                               gAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATAtt                      >  2:42331/1‑148 (MQ=255)
                                                                                                                                 aTACCGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATtgat                   <  6:159853/149‑1 (MQ=255)
                                                                                                                                         cTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTa            <  2:138081/148‑1 (MQ=255)
                                                                                                                                         cTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTAt           >  1:19133/1‑149 (MQ=255)
                                                                                                                                            cATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTAtt          <  8:124910/147‑1 (MQ=255)
                                                                                                                                               gatgatTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCggg      >  6:5025/1‑148 (MQ=255)
                                                                                                                                                  gatTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGCTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGATTATTGATGATCTTATTCGGGAAGc  >  3:1755/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGTGCGCCAATAAACGCTGGCAGGAATGAATGGTGAATATTGATGATCTTATTCGGGAAGC  >  NC_000913/1287792‑1288086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑