Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,355,673 A→G 8.5% S480P (TCT→CCT)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,355,6730AG 8.5% 133.2 / 3.8 47S480P (TCT→CCT) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (18/25);  new base G (2/2);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

AAACGGGCTAAGTACCAGACCTTTGATATCGTACCGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATC  >  NC_000913/1355527‑1355820
                                                                                                                                                  |                                                                                                                                                   
aaaCGGGCTAAGTACCAGACCTTTGATATCGTACCGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAaga                                                                                                                                                     >  3:112672/1‑147 (MQ=255)
        tAAGTACCAGACCTTTGATATCGTACCGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTa                                                                                                                                           >  6:196780/1‑149 (MQ=255)
                          tatCGTACCGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAAt                                                                                                                         >  6:14437/1‑149 (MQ=255)
                           atCGTACCGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCa                                                                                                                                       <  1:181705/134‑1 (MQ=255)
                           atCGTACCGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCa                                                                                                                                       >  2:181705/1‑134 (MQ=255)
                            tCGTACCGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAAttt                                                                                                                       >  1:139593/1‑149 (MQ=255)
                              gTACCGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCg                                                                                                                     <  5:196780/149‑1 (MQ=255)
                                  cGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCg                                                                                                                     <  5:80645/145‑1 (MQ=255)
                                  cGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCg                                                                                                                     >  6:80645/1‑145 (MQ=255)
                                       ggCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCAcc                                                                                                            <  7:96203/149‑1 (MQ=255)
                                             cAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTggg                                                                                                      >  5:174927/1‑149 (MQ=255)
                                             cAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTg                                                                                                        <  5:54024/147‑1 (MQ=255)
                                               aaCGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCg                                                                                                    <  7:50099/149‑1 (MQ=255)
                                                    aaTGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGtt                                                                                               >  4:98136/1‑149 (MQ=255)
                                                    aaTGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGtt                                                                                               >  5:135154/1‑149 (MQ=255)
                                                     aTGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTg                                                                                              <  5:14437/149‑1 (MQ=255)
                                                        acgacgCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTc                                                                                           <  3:153660/149‑1 (MQ=255)
                                                         cgacgCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTTTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCt                                                                                          >  5:234269/1‑149 (MQ=255)
                                                         cgacgCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCt                                                                                          >  3:10383/1‑149 (MQ=255)
                                                                   ggCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTTTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCgaatgaat                                                                                <  6:234269/149‑1 (MQ=255)
                                                                   ggCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCgaatgaat                                                                                >  6:88873/1‑149 (MQ=255)
                                                                       aaaTGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGcc                                                                            >  7:233362/1‑149 (MQ=255)
                                                                         aTGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCcgc                                                                          <  2:139593/149‑1 (MQ=255)
                                                                              cAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGc                                                                     <  8:136369/149‑1 (MQ=255)
                                                                                 ttCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATTCGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCa                                                                  <  1:181717/149‑1 (MQ=255)
                                                                                 ttCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGc                                                                                                     >  7:207184/1‑114 (MQ=255)
                                                                                 ttCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCAACAGTGTGc                                                                                                     <  8:207184/114‑1 (MQ=255)
                                                                                    cTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGt                                                                >  6:47597/1‑148 (MQ=255)
                                                                                    cTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGGTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTa                                                               <  8:233362/149‑1 (MQ=255)
                                                                                         ccAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAacgga                                                          <  6:174927/149‑1 (MQ=255)
                                                                                            gAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAacgcacg                                                        >  5:81947/1‑148 (MQ=255)
                                                                                              aTACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGgaa                                                     <  4:112672/149‑1 (MQ=255)
                                                                                                  tctGCGGTTCGTCATAGGCTTCAATACGCGGCGCCTGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCgaatga                                                                                  <  6:229404/116‑1 (MQ=255)
                                                                                                  tctGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCgaatga                                                                                  >  5:229404/1‑116 (MQ=255)
                                                                                                    tGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGaaa                                                 <  4:193800/147‑1 (MQ=255)
                                                                                                        cTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTcc                                           <  8:34316/149‑1 (MQ=255)
                                                                                                            gTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGt                                       <  7:2463/149‑1 (MQ=255)
                                                                                                                  ggCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTa                                  <  5:47597/148‑1 (MQ=255)
                                                                                                                   gCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCa                                                                  <  7:4358/115‑1 (MQ=255)
                                                                                                                   gCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCa                                                                  >  8:4358/1‑115 (MQ=255)
                                                                                                                    cTTCAATACGCGCCGCCAGCTGCTGCGAAGGGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCgg                                         >  6:190828/1‑139 (MQ=255)
                                                                                                                    cTTCAATACGCGCCGCCAGCTGCTGCGAAGGGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCgg                                         <  5:190828/139‑1 (MQ=255)
                                                                                                                          tACGCGCCGCCAGCTGCTGCGAAGGGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTg                                                                              >  3:56860/1‑96 (MQ=255)
                                                                                                                          tACGCGCCGCCAGCTGCTGCGAAGGGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTg                                                                              <  4:56860/96‑1 (MQ=255)
                                                                                                                                  gccAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAAcc                 <  6:135154/149‑1 (MQ=255)
                                                                                                                                             cGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGt        >  4:27519/1‑147 (MQ=255)
                                                                                                                                               aaGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGa    <  7:897/149‑1 (MQ=255)
                                                                                                                                               aaGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGa    <  4:201129/149‑1 (MQ=255)
                                                                                                                                                 gagagCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATc  <  5:88873/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AAACGGGCTAAGTACCAGACCTTTGATATCGTACCGATAGGCCTGCAAACGCAATGACGACGCCAGCGGCAAAATGGGCAATTCCTGCGCCAGAATACTCTGCGCTTCGTCATAGGCTTCAATACGCGCCGCCAGCTGCTGCGAAGAGAGCGCCTTACGCAATACGCTGTCGAATTTCGGATCGCACCAGTGGGCGAGGTTGGTCTGCGAATGAATTGCCGCGCAGCTCAGTAACGGACGGAAGAAACTGTCCGGGTCGTTACTGTCCGTCGCCCAACCGGATAACGTCAGATC  >  NC_000913/1355527‑1355820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑