Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,534,754 C→T 7.8% A244V (GCT→GTT)  nhoA → N‑hydroxyarylamine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,534,7540CT 7.8% 152.5 / 2.8 51A244V (GCT→GTT) nhoAN‑hydroxyarylamine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (21/26);  new base T (2/2);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

CGCAGTCGCATTTTCGCCATCATTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCG  >  NC_000913/1534607‑1534895
                                                                                                                                                   |                                                                                                                                             
cGCAGTCGCATTTTCGCCATCATTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCt                                                                                                                                              <  2:26286/149‑1 (MQ=255)
cGCAGTCCCATTTTCGCCATCATTTGCTGCAGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCt                                                                                                                                              <  4:35871/149‑1 (MQ=255)
       gCATTTTCGCCATCATTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGc                                                                                                                                       <  7:206491/149‑1 (MQ=255)
         aTTTTCGCCATCATTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCaa                                                                                                                                     <  5:69710/149‑1 (MQ=255)
             tCGCCATCATTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGaa                                                                                                                                  <  8:28158/148‑1 (MQ=255)
               gCCATCATTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAg                                                                                                                               >  1:1402/1‑149 (MQ=255)
                ccATCATTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGt                                                                                                                              >  8:111380/1‑149 (MQ=255)
                 aataatTTGCTGATGTGCCGCCATTTGCCGGACTGAGGCATGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATTGGCACGCGGTTGAGCAGCGAAATCTACCGGATGTGGCGTCTTTATATGCTGTGATGCAAGAACAGtt                                                                                                                             <  1:154709/145‑1 (MQ=255)
                  atcatTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGttt                                                                                                                            <  7:79187/149‑1 (MQ=255)
                   tcatTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTg                                                                                                                           >  6:151878/1‑149 (MQ=255)
                   tcatTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTg                                                                                                                           <  7:111380/149‑1 (MQ=255)
                     atTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGt                                                                                                                         <  2:1402/149‑1 (MQ=255)
                          cTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTgg                                                                                                                     <  2:198947/148‑1 (MQ=255)
                            gATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCATCATTATATGTTGTGATGc                                                                                                                                       >  6:11666/1‑128 (MQ=255)
                            gATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCATCATTATATGTTGTGATGc                                                                                                                                       <  5:8524/128‑1 (MQ=255)
                            gATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCATCATTATATGTTGTGATGc                                                                                                                                       >  6:8524/1‑128 (MQ=255)
                            gATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCATCATTATATGTTGTGATGc                                                                                                                                       <  5:11666/128‑1 (MQ=255)
                               gtgCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTg                                                                                                                <  2:86675/148‑1 (MQ=255)
                                tgCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGga                                                                                                              <  6:209893/149‑1 (MQ=255)
                                   cgccATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGTAACGCGGTGGAGCAGCTAAATCTACCGGATGTTGCTTCATTATATGCTGTGATTCAAGAACAGTTTGGTCTGGGAGTGgatga                                                                                                           >  6:66334/1‑149 (MQ=255)
                                      cATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGc                                                                                                        >  1:205592/1‑149 (MQ=255)
                                      cATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGc                                                                                                        <  5:151878/149‑1 (MQ=255)
                                        tttGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCg                                                                                                       >  7:38535/1‑148 (MQ=255)
                                           gCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGaaa                                                                                                    <  4:170607/148‑1 (MQ=255)
                                            ccGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAAc                                                                                                   <  3:200302/148‑1 (MQ=255)
                                             cGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGgatgat                                                                                                          <  4:125080/140‑1 (MQ=255)
                                             cGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGgatgat                                                                                                          >  3:125080/1‑140 (MQ=255)
                                             cGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACAt                                                                                                 >  4:6335/1‑149 (MQ=255)
                                               gACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATgg                                                                                               <  4:153501/149‑1 (MQ=255)
                                                      gcAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTAcc                                                                                        <  3:133922/149‑1 (MQ=255)
                                                           cTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGa                                                                                   <  7:51681/149‑1 (MQ=255)
                                                            tGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGAt                                                                                  >  3:158932/1‑149 (MQ=255)
                                                            tGACACTGACCAATTATCATTTTACCCATAATGAAAATGGGCACGCGGTGGAGCACAGAAATCTACCGGATCTGGCGTCAAAATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGTATGATGCGAAACATGGCATTACCGTGCAt                                                                                  >  4:37987/1‑149 (MQ=255)
                                                               cacTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAg                                                                               <  3:127268/149‑1 (MQ=255)
                                                                      cAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAgcgc                                                                        >  8:229185/1‑149 (MQ=255)
                                                                       aaTTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGACCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCTAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCt                                                                       <  5:232986/149‑1 (MQ=255)
                                                                           ttCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTg                                                                   >  5:97918/1‑149 (MQ=255)
                                                                              aTTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATg                                                                <  2:205592/149‑1 (MQ=255)
                                                                                     ccATTATGAAAATGGGCACGCGGTTGAGCAGCGAAATCTACCGTATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTTGTCTGGGCGGGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCTGCGt                                                         <  5:66334/149‑1 (MQ=255)
                                                                                     ccATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGt                                                         >  5:91927/1‑149 (MQ=255)
                                                                                         tATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGt                                                                                      <  5:203610/116‑1 (MQ=255)
                                                                                         tATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGt                                                                                      >  6:203610/1‑116 (MQ=255)
                                                                                             aaaaTGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACg                                                 >  7:139828/1‑149 (MQ=255)
                                                                                                      aCGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATTAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAg                                        >  8:239532/1‑149 (MQ=255)
                                                                                                                  agcGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAaataa                            <  8:38535/149‑1 (MQ=255)
                                                                                                                         tCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGt                     >  5:202367/1‑149 (MQ=255)
                                                                                                                           tACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGt                     >  4:69496/1‑147 (MQ=255)
                                                                                                                                gATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTg              >  3:202366/1‑149 (MQ=255)
                                                                                                                                         tCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATgcg     <  7:239532/149‑1 (MQ=255)
                                                                                                                                           atcatatGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATgcggc   >  4:115396/4‑149 (MQ=255)
                                                                                                                                             tataTGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATgcggcg  >  7:130303/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CGCAGTCGCATTTTCGCCATCATTTGCTGATGTGCCGCCATTTGCCGGACGGCGGCAAGCTGACACTGACCAATTTTCATTTTACCCATTATGAAAATGGGCACGCGGTGGAGCAGCGAAATCTACCGGATGTGGCGTCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCG  >  NC_000913/1534607‑1534895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑