Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,534,890 G→A 9.7% intergenic (+21/+75) nhoA → / ← yddE N‑hydroxyarylamine O‑acetyltransferase/PhzC‑PhzF family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,534,8900GA 9.7% 145.6 / 4.3 51intergenic (+21/+75)nhoA/yddEN‑hydroxyarylamine O‑acetyltransferase/PhzC‑PhzF family protein
Reads supporting (aligned to +/- strand):  ref base G (25/21);  new base A (2/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCGGT  >  NC_000913/1534744‑1535034
                                                                                                                                                  |                                                                                                                                                
tCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATgcg                                                                                                                                                <  7:239532/149‑1 (MQ=255)
  atcatatGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATgcggc                                                                                                                                              >  4:115396/4‑149 (MQ=255)
    tataTGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATgcggcg                                                                                                                                             >  7:130303/1‑148 (MQ=255)
           tgtgATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGcc                                                                                                                                     >  1:171119/1‑149 (MQ=255)
           tgtgATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGcc                                                                                                                                     >  4:65141/1‑149 (MQ=255)
             tgATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGcc                                                                                                                                     <  4:202366/147‑1 (MQ=255)
             tgATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGGGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATAAGGCGTTTACGcc                                                                                                                                     <  7:229185/147‑1 (MQ=255)
                 gCAAGCACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAATTAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                               >  7:85803/1‑149 (MQ=255)
                 gCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                               >  7:184779/1‑149 (MQ=255)
                  cAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                              <  4:158932/149‑1 (MQ=255)
                     gAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCa                                                                                                                           <  1:123080/149‑1 (MQ=255)
                             tGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTa                                                                                                                    <  3:6335/148‑1 (MQ=255)
                                         ggATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGa                                                                                                       >  8:126051/1‑149 (MQ=255)
                                           atgatgCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGAcc                                                                                                     <  6:91927/149‑1 (MQ=255)
                                            tgatgCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTTATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCt                                                                                                    >  5:161761/1‑149 (MQ=255)
                                             gatgCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCt                                                                                                    >  7:222552/1‑148 (MQ=255)
                                             gatgCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACACGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATAGATTATCCCGGTGGTAGACCTg                                                                                                   >  2:249378/1‑149 (MQ=255)
                                               tgCGAAACATGGCTTTACCGTGGATTAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGAc                                                                                                 <  3:37987/149‑1 (MQ=255)
                                                 cGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAg                                                                                               <  8:139828/149‑1 (MQ=255)
                                                  gAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAgg                                                                                              >  7:58663/1‑149 (MQ=255)
                                                  gAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAgg                                                                                              >  8:78537/1‑149 (MQ=255)
                                                       aTGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAg                                                                                         >  7:207631/1‑149 (MQ=255)
                                                       aTGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATAAGGCGttt                                                                                                                                          <  7:151507/100‑3 (MQ=255)
                                                       aTGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATAAGGCGttt                                                                                                                                          <  5:220373/100‑3 (MQ=255)
                                                       aTGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATAAGGCGttt                                                                                                                                          >  8:151507/1‑98 (MQ=255)
                                                       aTGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATAAGGCGttt                                                                                                                                          >  6:220373/1‑98 (MQ=255)
                                                          gCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAgcgc                                                                                      <  3:69496/149‑1 (MQ=255)
                                                           cTTTACCGTGGATGAGTTAGCGCTGGTGATGGAGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAgcgc                                                                                      >  2:50273/1‑148 (MQ=255)
                                                            tttACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAg                                                                                               >  3:195279/1‑138 (MQ=255)
                                                            tttACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAg                                                                                               <  4:195279/138‑1 (MQ=255)
                                                            gttACCGTGGATGAGTTAGCGCTGCTGATGGCGGCGTTTGATCCGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGGTGTAGACCTGACAGGCGAAGCGCAt                                                                                    <  6:97918/148‑1 (MQ=255)
                                                               aCCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCa                                                                                  <  8:130303/148‑1 (MQ=255)
                                                                     gATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAAAGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACg                                                                           >  3:117293/1‑149 (MQ=255)
                                                                           ttAGCGCTGGTGATGGCGGCGTTTTATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCa                                                                     <  6:161761/149‑1 (MQ=255)
                                                                           ttAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCa                                                                     <  8:222552/149‑1 (MQ=255)
                                                                           ttAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCa                                                                     <  2:171119/149‑1 (MQ=255)
                                                                                          gcggcgTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCa                                                      >  8:41081/1‑149 (MQ=255)
                                                                                           cggcgTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCAt                                                     >  5:211717/1‑149 (MQ=255)
                                                                                                tttGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGc                                                <  6:202367/149‑1 (MQ=255)
                                                                                                        gCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAAc                                         >  2:62519/1‑148 (MQ=255)
                                                                                                        gCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGGTTCAGAGTTCAATTGCCCATTCGGCATGAAAc                                         >  2:176339/1‑148 (MQ=255)
                                                                                                                    gggAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCAtt                                                    >  6:39034/1‑125 (MQ=255)
                                                                                                                    gggAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCAtt                                                    <  5:39034/125‑1 (MQ=255)
                                                                                                                    gggAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCtg                            >  3:114340/1‑149 (MQ=255)
                                                                                                                      gAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAAtt                                                           >  3:197271/1‑116 (MQ=255)
                                                                                                                      gAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAAtt                                                           <  4:197271/116‑1 (MQ=255)
                                                                                                                      gAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCtgtg                          >  2:83285/1‑149 (MQ=255)
                                                                                                                       aaaaTAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGc                         <  8:207631/149‑1 (MQ=255)
                                                                                                                            aatTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGa                     >  4:170817/1‑148 (MQ=255)
                                                                                                                                    tCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGAc            <  7:126051/149‑1 (MQ=255)
                                                                                                                                     cAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACt           >  8:218373/1‑149 (MQ=255)
                                                                                                                                        gTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGaaa                                          >  1:141028/1‑115 (MQ=255)
                                                                                                                                        gTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGaaa                                          <  2:141028/115‑1 (MQ=255)
                                                                                                                                              ggATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCggt  <  8:85803/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCATTATATGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCGGT  >  NC_000913/1534744‑1535034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑