| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 755,277 | +GG | coding (101/390 nt) | sdhC → | succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 755,275 | 1 | . | G | 97.6% | 144.4 / ‑2.9 | 42 | coding (99/390 nt) | sdhC | succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (18/23); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
| * | NC_000913 | 755,275 | 2 | . | G | 97.6% | 145.8 / ‑2.9 | 42 | coding (99/390 nt) | sdhC | succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (18/23); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCC‑‑GGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTA > NC_000913/755134‑755411 || gTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCC‑‑GGggtgt > 4:24213/1‑148 (MQ=255) cccAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCAc < 2:117138/149‑1 (MQ=255) cccAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCAc < 2:147291/149‑1 (MQ=255) cccAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCAc < 7:44712/149‑1 (MQ=255) gggAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCttt < 2:177775/149‑1 (MQ=255) aataatAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTttgtt > 5:3519/1‑149 (MQ=255) ataataAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTttgttg < 8:149331/149‑1 (MQ=255) gAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCt > 1:77172/1‑136 (MQ=255) gAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCt < 2:77172/136‑1 (MQ=255) gCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATcc > 6:89417/1‑149 (MQ=255) ttCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCC‑‑ggg < 1:153716/105‑2 (MQ=255) ttCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCC‑‑ggg > 2:153716/1‑104 (MQ=255) tCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTgg > 1:80990/1‑149 (MQ=255) tgAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCtt < 6:3519/149‑1 (MQ=255) aaaaCAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATCGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTcccc < 7:88735/149‑1 (MQ=255) aaaaCAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAg > 7:28263/1‑106 (MQ=255) aaaaCAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAg < 8:28263/106‑1 (MQ=255) aaaaCAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTcccc > 8:88735/1‑149 (MQ=255) cAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGaa < 2:80990/149‑1 (MQ=255) cTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTc > 6:88291/1‑148 (MQ=255) gTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCtgt > 8:124900/1‑108 (MQ=255) gTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCtgt < 7:124900/108‑1 (MQ=255) aTCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAg < 5:89417/149‑1 (MQ=255) aCAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGAt > 6:127395/1‑149 (MQ=255) aCCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCctgc > 6:39622/1‑87 (MQ=255) aCCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCctgc < 5:39622/87‑1 (MQ=255) aCCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATg < 3:24213/149‑1 (MQ=255) tCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCC‑‑g > 8:83746/1‑48 (MQ=255) tCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCC‑‑g < 7:83746/48‑1 (MQ=255) cGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGc < 5:88291/149‑1 (MQ=255) cccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAg > 1:137292/1‑111 (MQ=255) cccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAg < 2:137292/111‑1 (MQ=255) ccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCctc > 7:83043/1‑97 (MQ=255) ccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCctc < 8:83043/97‑1 (MQ=255) ccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTcc > 2:133810/1‑103 (MQ=255) ccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTcc < 1:133810/103‑1 (MQ=255) ccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCg > 8:32161/1‑149 (MQ=255) cACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTgg > 4:74037/1‑82 (MQ=255) cACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTgg < 3:74037/82‑1 (MQ=255) ggCGATAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAAtt < 7:32161/149‑1 (MQ=255) aTAGCGTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATc < 5:127395/149‑1 (MQ=255) gTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCttcttc < 5:135610/132‑1 (MQ=255) gTCCATTCTCCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCttcttc > 6:135610/1‑132 (MQ=255) tcCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTAGCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTggg < 8:16905/143‑1 (MQ=255) tcCATCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTAACAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTggg > 7:16905/1‑143 (MQ=255) aTCGCGTTTCCGGGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTa > 4:9205/1‑149 (MQ=255) || GTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTCATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCC‑‑GGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTA > NC_000913/755134‑755411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |