Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,807,3170CT21.1% 73.1 / 14.9 38intergenic (+18/‑83)pfkB/ydiZ6‑phosphofructokinase II/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (15/15);  new base T (4/4);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACT  >  NC_000913/1807170‑1807462
                                                                                                                                                   |                                                                                                                                                 
aGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCa                                                                                                                                                  >  3:94148/1‑149 (MQ=255)
     aTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                                                                                                             <  7:90158/149‑1 (MQ=255)
        ccTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggggg                                                                                                                                           <  8:67574/148‑1 (MQ=255)
                                   gCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGt                                                                                                               >  5:44237/1‑149 (MQ=255)
                                         cTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTcc                                                                                                         <  4:94148/149‑1 (MQ=255)
                                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAc                                                                                                                                  >  8:80574/1‑112 (MQ=255)
                                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAc                                                                                                                                  <  7:80574/112‑1 (MQ=255)
                                                            acTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAt                                                                                      >  7:95189/1‑149 (MQ=255)
                                                                aaTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGa                                                                                  >  6:147777/1‑149 (MQ=255)
                                                                  tCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGAt                                                                                 >  8:130682/1‑148 (MQ=255)
                                                                        aaCACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAg                                                                          >  2:48279/1‑149 (MQ=255)
                                                                         acacGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgg                                                                         <  8:95189/149‑1 (MQ=255)
                                                                          cacGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAggg                                                                        <  7:130682/149‑1 (MQ=255)
                                                                            cGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCCTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGt                                                                      <  2:37662/149‑1 (MQ=255)
                                                                             gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGt                                                                      >  6:93327/1‑148 (MQ=255)
                                                                                    cTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATg                                                              <  6:44237/149‑1 (MQ=255)
                                                                                    cTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATg                                                              <  1:48279/149‑1 (MQ=255)
                                                                                           gACGATACGCAAAAAATTTACGCTTACATTTCCCGCTAACAAAAACATTCACCCAGCATTGGGGGAATCATCACAAACCTGTCGGCAAAGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTg                                                       >  5:90343/1‑149 (MQ=255)
                                                                                                   gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTc                                                                                                                         >  3:51904/1‑75 (MQ=255)
                                                                                                   gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTc                                                                                                                         <  4:51904/75‑1 (MQ=255)
                                                                                                     aaaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAggg                                                                        >  1:89730/1‑122 (MQ=255)
                                                                                                     aaaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAggg                                                                        <  2:89730/122‑1 (MQ=255)
                                                                                                     aaaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGt                                                                      >  7:13527/1‑124 (MQ=255)
                                                                                                     aaaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGt                                                                      <  8:13527/124‑1 (MQ=255)
                                                                                                          aTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGtt                                        <  5:93327/149‑1 (MQ=255)
                                                                                                                      cTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCTTCAACGCGTTTCTCCGACTATGCTCAAAATTCATGTGATAACAAAGGTTTTAACTATGGCCAGTGGCGATCTTGTACGTTATTTCATATCCg                            <  6:90343/149‑1 (MQ=255)
                                                                                                                                   aaaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATa                                                                                >  4:10138/1‑84 (MQ=255)
                                                                                                                                   aaaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATa                                                                                <  3:10138/84‑1 (MQ=255)
                                                                                                                                     gatgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATg                                                              >  4:86652/5‑100 (MQ=38)
                                                                                                                                     gatgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATg                                                              <  3:86652/96‑1 (MQ=38)
                                                                                                                                     gatgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa               >  5:120051/5‑147 (MQ=255)
                                                                                                                                     gatgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa               <  6:120051/143‑1 (MQ=255)
                                                                                                                                     aaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAg              >  7:8698/1‑148 (MQ=255)
                                                                                                                                      atgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGc                                                     >  2:149197/4‑108 (MQ=255)
                                                                                                                                      atgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGc                                                     <  1:149197/105‑1 (MQ=255)
                                                                                                                                        gaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa               >  5:128567/2‑144 (MQ=255)
                                                                                                                                        gaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa               <  6:128567/143‑1 (MQ=255)
                                                                                                                                                cAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACt  >  7:16432/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACT  >  NC_000913/1807170‑1807462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑