| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,891,502 | 0 | C | G | 20.7% | 64.9 / 14.3 | 29 | intergenic (+19/‑113) | tnaB/mdtL | tryptophan transporter of low affinity/multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (11/12); new base G (3/3); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCT > NC_000913/3891363‑3891646 | cAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTgg < 6:127929/149‑1 (MQ=255) tGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGc < 8:112708/149‑1 (MQ=255) tCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTAt < 3:127838/146‑1 (MQ=255) tCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTAt > 4:127838/1‑146 (MQ=255) cGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttctt < 8:63084/117‑1 (MQ=255) cGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttctt > 7:63084/1‑117 (MQ=255) cGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATgg > 8:26100/1‑148 (MQ=255) tCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttct < 5:105434/107‑1 (MQ=255) tCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttct > 6:105434/1‑107 (MQ=255) aTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCa < 3:37266/114‑1 (MQ=255) aTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCa > 4:37266/1‑114 (MQ=255) ttCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt > 3:60317/1‑149 (MQ=255) tctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGctt < 8:133833/99‑1 (MQ=255) tctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGctt > 7:133833/1‑99 (MQ=255) tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACtt < 5:53338/119‑1 (MQ=255) tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACtt > 6:53338/1‑119 (MQ=255) tGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctcc < 4:60317/147‑1 (MQ=255) ccGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCtctc < 8:130482/97‑1 (MQ=255) ccGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCtctc > 7:130482/1‑97 (MQ=255) ccGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGt < 7:26100/149‑1 (MQ=255) ttttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttctt < 1:83598/84‑1 (MQ=255) ttttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttctt > 2:83598/1‑84 (MQ=255) ttACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGc < 6:1389/84‑1 (MQ=255) ttACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGc > 5:1389/1‑84 (MQ=255) gCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCttt < 1:67916/148‑1 (MQ=255) ttCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTg > 7:94503/1‑147 (MQ=255) aagaagCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctcc < 4:43087/76‑1 (MQ=38) aagaagCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctcc > 3:43087/1‑76 (MQ=38) gaagCCAGCCATTTGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCt > 2:63336/1‑66 (MQ=255) cagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCt < 8:94503/149‑1 (MQ=255) | CAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCT > NC_000913/3891363‑3891646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |