Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	696,793	T→A	7.1%	noncoding (38/75 nt)	glnW ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,793	0	T	A	7.1%	208.4 / 3.4	67	noncoding (38/75 nt)	glnW	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base T (28/33);  new base A (3/2);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAG  >  NC_000913/696647‑696891                                                                                                                                                   |                                                                                                   tCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTAt                                                                                                  >  1:191411/1‑149 (MQ=255) tCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTAt                                                                                                  >  7:96227/1‑149 (MQ=255)  cGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTAt                                                                                                  <  8:524694/148‑1 (MQ=255)    aTACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaa                                                                                               <  8:83658/149‑1 (MQ=255)       cATTCAGAATTTGGTGGCTACGACGGGATTCTAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGGACCTGGATTCGAACCAGGGAATGCCGGTATCaaaaa                                                                                            <  3:140829/149‑1 (MQ=255)        aTTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAc                                                                                           <  8:96227/149‑1 (MQ=255)        aTTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAc                                                                                           <  2:191411/149‑1 (MQ=255)         ttCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc                                                                                          >  8:56867/1‑149 (MQ=255)            aGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                                                       >  6:81403/1‑149 (MQ=255)                   ggtggCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTa                                                                                 <  7:56867/148‑1 (MQ=255)                    gtggCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTAc                                                                                <  2:83467/148‑1 (MQ=255)                     tggCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCg                                                                              >  3:498407/1‑149 (MQ=255)                         tACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACCGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTTGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTg                                                                          >  7:66841/1‑149 (MQ=255)                           cgacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTg                                                                          <  6:184386/147‑1 (MQ=255)                            gacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                                                        >  7:321918/1‑148 (MQ=255)                            gacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                                                        >  1:298477/1‑148 (MQ=255)                            gacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACAAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                                                        >  2:231929/1‑148 (MQ=255)                                  aTTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATAccc                                                                 <  5:81403/149‑1 (MQ=255)                                      gAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTgg                                                                         >  1:466946/1‑137 (MQ=255)                                      gAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTgg                                                                         <  2:466946/137‑1 (MQ=255)                                       aaCCTGTGACCCCATCATTATGACTGATGAGCTCAAACCAAGTGAGCGACGTAGCCAGCTTGTTTCTGCGAGGGCTGGGGTCGCGGGATTCGAACCAGGGAATGCCGGTAACCCAAACAGGTGACTGAACGCGTGGCGAGACCCCaag                                                             >  1:353603/1‑147 (MQ=16)                                             tgACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCggg                                                      >  2:272466/1‑149 (MQ=255)                                              gACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGATCGAGGAAATGCCCTAATCAAAAAACAGTGCCTTACCGCTTCTCGATTCACCAATAAcctg                                                       >  1:151934/1‑145 (MQ=25)                                                 cccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGt                                                  <  7:74192/149‑1 (MQ=255)                                                 cccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGt                                                  >  4:392026/1‑149 (MQ=255)                                                  ccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCgg                                                       >  2:49997/1‑143 (MQ=255)                                                  ccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCgg                                                       <  1:49997/143‑1 (MQ=255)                                                     tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGt                                                  >  3:141032/1‑145 (MQ=255)                                                     tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGt                                                  <  4:141032/145‑1 (MQ=255)                                                     tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc                                              >  5:389630/1‑149 (MQ=255)                                                     tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc                                              >  5:363310/1‑149 (MQ=255)                                                     tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc                                              >  1:16490/1‑149 (MQ=255)                                                     tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc                                              <  4:498407/149‑1 (MQ=255)                                                     tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc                                              <  1:231929/149‑1 (MQ=255)                                                     tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc                                              >  3:154422/1‑149 (MQ=255)                                                       atTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGaa                                               <  6:517293/146‑1 (MQ=255)                                                       atTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGaa                                               >  5:517293/1‑146 (MQ=255)                                                              gTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTc                                     <  3:392026/149‑1 (MQ=255)                                                                    tgCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaagaa                               <  6:389630/149‑1 (MQ=255)                                                                    tgCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaaga                                <  8:321918/148‑1 (MQ=255)                                                                      ctctAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTAc                                       <  7:164297/139‑1 (MQ=255)                                                                      ctctAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTAc                                       >  8:164297/1‑139 (MQ=255)                                                                      ctctAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAagaaga                             <  1:272466/149‑1 (MQ=255)                                                                       tctAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGa                                                                      >  1:267039/1‑107 (MQ=255)                                                                       tctAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGa                                                                      <  2:267039/107‑1 (MQ=255)                                                                           aCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGa                                                                      >  4:97532/1‑103 (MQ=255)                                                                           aCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGa                                                                      <  3:97532/103‑1 (MQ=255)                                                                           aCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACt                                      <  1:422242/135‑1 (MQ=255)                                                                           aCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACt                                      >  2:422242/1‑135 (MQ=255)                                                                           aCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaag                                 >  8:388015/1‑140 (MQ=255)                                                                           aCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaag                                 <  7:388015/140‑1 (MQ=255)                                                                           aCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATgg                          <  2:16490/147‑1 (MQ=255)                                                                           aCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGc                         <  4:154422/148‑1 (MQ=255)                                                                            ccAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTg                       <  2:63440/149‑1 (MQ=255)                                                                               aCTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAATAAGATGGCTgggg                    <  6:363310/149‑1 (MQ=255)                                                                                    gCTGCGTAGCGAGTTTGTTTCTTAGATGGCTGGGGTACCTTGTTTCGATCCAGGGAATGCCGGGATCAAAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCt               <  7:234237/149‑1 (MQ=25)                                                                                      tACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATa                                                                    >  8:278795/1‑94 (MQ=255)                                                                                      tACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATa                                                                    <  7:278795/94‑1 (MQ=255)                                                                                                 aTTGTTTCTTCGATGTCGTGGGTACCTTGATGTGATCCAGGTAATGCCTGTATCAATACCCGGTTCCTTAACGCTTGTCGTTACCCAAATAACCGCGCGTTGAACCGATTACTCGTATTAGATGGCTGGGGTACCTGGATTCGAACCAg  <  8:66841/149‑1 (MQ=255)                                                                                                        cttcGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGt                                                                                                    <  4:384747/44‑1 (MQ=40)                                                                                                        cttcGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGt                                                                                                    >  3:384747/1‑44 (MQ=40)                                                                                                                    gggTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTc                                     >  4:348840/1‑95 (MQ=255)                                                                                                                    gggTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTc                                     <  3:348840/95‑1 (MQ=255)                                                                                                                       tacgaggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATgg                          >  6:423855/6‑103 (MQ=17)                                                                                                                       tacgaggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATgg                          <  5:423855/98‑1 (MQ=17)                                                                                                                                  gAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCt               <  6:56602/103‑1 (MQ=255)                                                                                                                                  gAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCt               >  5:56602/1‑103 (MQ=255)                                                                                                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTg                       <  2:261589/91‑1 (MQ=255)                                                                                                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTg                       >  1:261589/1‑91 (MQ=255)                                                                                                                                                   |                                                                                                   TCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAG  >  NC_000913/696647‑696891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑