Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,970 A→G 7.5% intergenic (+368/+246) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9700AG 7.5% 177.2 / 4.7 66intergenic (+368/+246)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base A (26/35);  new base G (3/2);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGT  >  NC_000913/3269823‑3270106
                                                                                                                                                   |                                                                                                                                        
aaTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAg                                                                                                                                         >  1:440132/1‑149 (MQ=255)
 aTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAg                                                                                                                                         <  7:462271/148‑1 (MQ=255)
    aaGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCgg                                                                                                                                      <  4:400774/148‑1 (MQ=255)
      gcgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGtt                                                                                                                                   >  8:451296/1‑149 (MQ=255)
        gcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGtttt                                                                                                                                 <  6:429924/149‑1 (MQ=255)
          aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGACGTGATTGAAATTTGTGACGAGGCAAAAGTAAAAAACCGGGGGAGCGGgttgtt                                                                                                                                >  2:236055/1‑148 (MQ=18)
          aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttttt                                                                                                                                <  2:389949/148‑1 (MQ=255)
           cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                              <  3:179113/149‑1 (MQ=255)
           cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCCCCGAAGCGGGTTTTTAc                                                                                                                              <  4:129824/149‑1 (MQ=255)
            tCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTAc                                                                                                                              >  2:66271/1‑148 (MQ=255)
            tCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                             >  1:510965/1‑149 (MQ=255)
            tCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGTAAAAACACGCCGAAGAGGGTTTTTACg                                                                                                                             >  1:69792/1‑149 (MQ=255)
              aacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTAc                                                                                                                              <  1:66271/146‑1 (MQ=255)
              aacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                            <  7:451296/148‑1 (MQ=255)
                caaTCTGAATCCCGCATTAACGGTTTATTACGTACATAAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTTTTTGATGTTTCTTCCCGTACAAAAGTAAATACCTTCCGAAGCGGGTTTTTACGTaaa                                                                                                                         <  2:456182/149‑1 (MQ=17)
                caaTCTGAAACCCGCCGTAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAATCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTAATTCATATTTATGCCACTCCAAAAGTAAAAACCAAAATAAGCGTGTTTTTAAGTaa                                                                                                                          >  6:262871/1‑148 (MQ=17)
                 aaTCTGAAACCCGCCGGAACGGTTTATTTCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                                        <  4:50964/149‑1 (MQ=255)
                 aaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTGATTCATATATGTTCCGCTCCAAAATTAATAACCCTGCGAGCCGTGTTATTAAGTaag                                                                                                                         >  8:145118/1‑147 (MQ=17)
                 aaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                                        <  1:244651/149‑1 (MQ=255)
                 aaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                                        <  2:440132/149‑1 (MQ=255)
                  aTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAc                                                                                                                       <  3:173381/149‑1 (MQ=255)
                          cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                               <  5:129126/149‑1 (MQ=255)
                          cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGGAAAAACCCGCCGAAGCGGGTTTTTACGTAAAagagcggaa                                                                                                                >  3:76941/1‑143 (MQ=255)
                                ggAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                             >  5:65913/1‑129 (MQ=255)
                                ggAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                             <  6:65913/129‑1 (MQ=255)
                                 gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCAGCTTTCTTTTGGGTATAGTGTAGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCTCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                                        <  5:262871/149‑1 (MQ=255)
                                   aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                                                                                                      <  2:510965/149‑1 (MQ=255)
                                     ggattattaCGTACATCACGTAAAACTGACCGATAAGCCGCTTTCATTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                    <  2:69792/146‑1 (MQ=255)
                                         tattaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGTGTCGTGGACATTCATTAATAATTATGCCCCTCCAAAACAAAAAACACCCAGAAACGGGTTTTTACGTAAAACAGGTGAAACTGACCGAAAAGcgg                                                                                                >  4:111583/1‑147 (MQ=14)
                                           ttaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCgg                                                                                                                                      <  4:393328/109‑1 (MQ=255)
                                           ttaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCgg                                                                                                                                      >  3:393328/1‑109 (MQ=255)
                                                   aTCATGTAAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCATTCATCTTTCTGCCCCTCCAAAAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAGACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                      <  7:145118/149‑1 (MQ=11)
                                                        gTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAc                                                                                                                       >  5:361677/1‑111 (MQ=38)
                                                        gTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAc                                                                                                                       <  6:361677/111‑1 (MQ=38)
                                                           acaCTTACCGATAAGCCGCTTGCTTTTGTGGTTCGTGTCGCGGAATGTGTTTCTTCTATCTGCCATTCCACAATTACAAACCCGCCGAATCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg                                                                               <  8:156190/146‑1 (MQ=2)
                                                            aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                               <  6:529383/99‑1 (MQ=33)
                                                            aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                               >  5:529383/1‑99 (MQ=33)
                                                            aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATa                                                                                                     <  7:25413/125‑1 (MQ=35)
                                                            aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATa                                                                                                     >  8:25413/1‑125 (MQ=35)
                                                                    gATAAGCCGCGTTCTTTTGGGGATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAATAGTAAAAACCCGCCGTAGCGTGTTTTTACTTAACACTGGTGTAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                     <  2:495339/133‑1 (MQ=11)
                                                                                              tgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                       <  6:122681/105‑1 (MQ=35)
                                                                                                    ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattc                                                             <  2:126763/125‑1 (MQ=38)
                                                                                                    ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattc                                                             >  1:126763/1‑125 (MQ=38)
                                                                                                            attcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTg                                                    <  8:382491/126‑1 (MQ=38)
                                                                                                             ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                      >  7:273732/1‑107 (MQ=17)
                                                                                                             ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGtcattca                                                            >  4:92993/1‑117 (MQ=25)
                                                                                                                atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcatt                                                              <  2:510803/112‑1 (MQ=255)
                                                                                                                atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcatt                                                              >  1:510803/1‑112 (MQ=39)
                                                                                                                atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCt                                                     >  4:94962/1‑121 (MQ=38)
                                                                                                                atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCt                                                     <  3:94962/121‑1 (MQ=38)
                                                                                                                         ccccTCCAAAAGTAAAAACCCGCATAAGCGGGTTTTTACGTAAAAAAGATTAAACTGACCCATAAGCATCTTAATTTTGTCTATAGATTAGTTGAAATACATTCAAAATTATTCAAATACAAAAAAAAAAACCCGCCTAGGCGGGtttt                >  2:148167/1‑149 (MQ=255)
                                                                                                                           ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                <  7:35648/83‑1 (MQ=38)
                                                                                                                           ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                >  8:35648/1‑83 (MQ=38)
                                                                                                                            cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTg                                                    >  8:385076/1‑110 (MQ=40)
                                                                                                                            cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTg                                                    <  7:385076/110‑1 (MQ=40)
                                                                                                                            cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt               <  8:268078/147‑1 (MQ=37)
                                                                                                                            cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGGGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt               >  7:268078/1‑147 (MQ=37)
                                                                                                                            cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCg                                                                          <  1:294685/88‑1 (MQ=18)
                                                                                                                            cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCg                                                                          >  2:294685/1‑88 (MQ=18)
                                                                                                                             tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                    >  8:91667/1‑61 (MQ=255)
                                                                                                                             tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCttt                                                       <  2:535334/106‑1 (MQ=40)
                                                                                                                             tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCttt                                                       >  1:535334/1‑106 (MQ=40)
                                                                                                                             tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                <  1:274219/145‑1 (MQ=37)
                                                                                                                             tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                >  2:274219/1‑145 (MQ=37)
                                                                                                                                     tAAAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg    >  7:287888/1‑149 (MQ=255)
                                                                                                                                       aaaaCCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt  <  8:287888/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
AATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGT  >  NC_000913/3269823‑3270106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: