Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,760	Δ1 bp	coding (854/1767 nt)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,758	0	G	.	100.0%	123.9 / NA	28	coding (852/1767 nt)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/15);  total (13/15)

TGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGG  >  NC_000913/756613‑756892                                                                                                                                                  |                                                                                                                                       tGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCggcc                                                                                                                                     <  2:116623/149‑3 (MQ=255)  ggAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCggccg                                                                                                                                    >  2:174668/1‑146 (MQ=255)   gAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCggccgt                                                                                                                                   >  5:54322/1‑145 (MQ=255)           gCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGCTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGt                                                                                                                          >  6:82655/1‑149 (MQ=255)                 ccACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGTAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTgcgc                                                                                                                     <  8:138165/148‑1 (MQ=255)                    cccGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTc                                                                                                                 >  1:174317/1‑149 (MQ=255)                      cGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGACGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCca                                                                                                               <  3:101620/149‑1 (MQ=255)                          cGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGCTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCcatcat                                                                                                           <  5:82655/149‑1 (MQ=255)                            gCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGa                                                                                                         >  3:78365/1‑149 (MQ=255)                                        cGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGt                                                                                              >  1:101849/1‑148 (MQ=255)                                           gCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAg                                                                                          <  1:174668/149‑1 (MQ=255)                                                 tGGTTACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTcc                                                                                                                <  4:130143/121‑1 (MQ=255)                                                      aCCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCtgt                                                                               >  6:131082/1‑149 (MQ=255)                                                      aCCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCtgt                                                                               >  1:50526/1‑149 (MQ=255)                                                           aGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATgg                                                                          <  2:50526/149‑1 (MQ=255)                                                                 ccGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGAt                                                                                                        <  8:116815/113‑1 (MQ=255)                                                                 ccGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTg                                                                    <  6:54322/149‑1 (MQ=255)                                                                      gtgAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGc                                                               >  1:37592/1‑149 (MQ=255)                                                                           ggCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTcgcg                                                                                    <  7:82791/123‑1 (MQ=255)                                                                           ggCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTcgcg                                                                                    >  8:82791/1‑123 (MQ=255)                                                                                       ctgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCaaaactga                                                <  2:174317/147‑1 (MQ=255)                                                                                          aacaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTc                                           >  4:127976/1‑149 (MQ=255)                                                                                            caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGa                                         <  2:37592/149‑1 (MQ=255)                                                                                            caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGa                                         <  4:78365/149‑1 (MQ=255)                                                                                                 aTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCAcc                                    >  4:115827/1‑149 (MQ=255)                                                                                                    gCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCt                                   <  2:101849/147‑1 (MQ=255)                                                                                                                                   cGCCAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCggg  >  8:95779/1‑149 (MQ=255)                                                                                                                                      cAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaactgaaa                                              <  2:183316/102‑1 (MQ=255)                                                                                                                                      cAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCgaaactgaaa                                              >  1:183316/1‑102 (MQ=255)                                                                                                                                      cAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAGCTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCg           <  6:52449/137‑1 (MQ=255)                                                                                                                                      cAAAGACCTGGC‑GGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAGCTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCg           >  5:52449/1‑137 (MQ=255)                                                                                                                                                  |                                                                                                                                       TGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGG  >  NC_000913/756613‑756892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑