Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,231,603 Δ1 bp 6.1% coding (418/1269 nt) umuC → translesion error‑prone DNA polymerase V subunit; DNA polymerase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,231,6030G. 6.1% 276.4 / 10.4 82coding (418/1269 nt)umuCtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity
Reads supporting (aligned to +/- strand):  ref base G (36/40);  new base . (2/3);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTG  >  NC_000913/1231457‑1231747
                                                                                                                                                   |                                                                                                                                                
tATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGcc                                                                                                                                                >  7:214804/1‑149 (MQ=255)
 aTCGCCACGCGACGAGATTTACACTATTGATGAGCCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTCGTGTGGGGATCGcc                                                                                                                                                >  2:403380/1‑148 (MQ=255)
  tCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGcc                                                                                                                                                >  3:80409/1‑147 (MQ=255)
  gcGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTTCGATCTGCCAGGGGGGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACACCGTACCCATCTTACTGTTGGTGTGGGGATCGCCCa                                                                                                                                              <  8:80841/148‑1 (MQ=255)
     ccccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGAc                                                                                                                                           >  5:136041/1‑149 (MQ=255)
      cccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGTATCGCCCAGAc                                                                                                                                           >  6:53141/1‑148 (MQ=255)
       ccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGAcc                                                                                                                                          >  5:76340/1‑148 (MQ=255)
          cgTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaaca                                                                                                                                      <  3:171118/149‑3 (MQ=255)
            tCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACg                                                                                                                                    >  3:87088/1‑149 (MQ=255)
               agaTTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTg                                                                                                                                 <  7:95693/149‑1 (MQ=255)
                gaTTTACAGTATTGATGAGGCTTTCTGCGATCTGACAGGTGTGCGTTATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTg                                                                                                                                 <  6:365762/148‑1 (MQ=255)
                    tACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaaca                                                                                                                                      <  7:109343/139‑3 (MQ=255)
                    tACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaaca                                                                                                                                      >  8:109343/1‑137 (MQ=255)
                     aCAGTATTGATGCGGCATTCTGCGATCTGACAGGGGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTaa                                                                                                                            <  1:273691/148‑1 (MQ=255)
                        gTATTGATGAGGCATTCTGCGAGCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCtt                                                                                                                        <  8:212420/149‑1 (MQ=255)
                                 aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                <  2:250418/148‑1 (MQ=255)
                                 aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                <  8:182337/148‑1 (MQ=255)
                                 aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                <  6:76340/148‑1 (MQ=255)
                                  ggCATTCTGCGATCTGACATGTGTGCGTAATTGTCGCGATCTGACTGATTTTGTCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATg                                                                                                              <  8:1206/149‑1 (MQ=255)
                                  ggCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaacag                                                                                                                                     <  4:55894/126‑4 (MQ=255)
                                  ggCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaacag                                                                                                                                     >  3:55894/1‑123 (MQ=255)
                                   gCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACATCGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGc                                                                                                             <  2:346421/149‑1 (MQ=255)
                                   gCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGc                                                                                                             >  3:21940/1‑149 (MQ=255)
                                    cATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACTGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCg                                                                                                            <  1:253696/149‑1 (MQ=255)
                                      ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATc                                                                                                                 <  3:59393/142‑1 (MQ=255)
                                      ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATc                                                                                                                 >  4:59393/1‑142 (MQ=255)
                                      ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                <  3:81772/143‑1 (MQ=255)
                                      ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                >  4:81772/1‑143 (MQ=255)
                                        cTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCa                                                                                                         >  2:271203/1‑148 (MQ=255)
                                         tGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaa                                                                                                        >  6:207271/1‑148 (MQ=255)
                                             aTCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa                                                                                                   >  2:300577/1‑149 (MQ=255)
                                                  aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa                                                                                                   <  3:266965/144‑1 (MQ=255)
                                                  aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa                                                                                                   <  8:339729/144‑1 (MQ=255)
                                                  aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAAt                                                                                                  <  6:323158/145‑1 (MQ=255)
                                                  aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATg                                                                                                 <  5:207271/146‑1 (MQ=255)
                                                  aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCa                                                                                              <  1:300577/149‑1 (MQ=255)
                                                   cAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAg                                                                                             <  1:403380/149‑1 (MQ=255)
                                                    aGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGc                                                                                            <  8:219964/149‑1 (MQ=255)
                                                         tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                <  5:344899/124‑1 (MQ=255)
                                                         tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                                >  6:344899/1‑124 (MQ=255)
                                                         tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAg                                                                                       >  5:271007/1‑149 (MQ=255)
                                                           cGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGAc                                                                                     >  8:318620/1‑149 (MQ=255)
                                                             tAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgg                                                                                   >  8:353210/1‑149 (MQ=255)
                                                               aTTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggt                                                                                 <  8:214804/149‑1 (MQ=255)
                                                                ttGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtg                                                                                <  2:189970/149‑1 (MQ=255)
                                                                   tCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtggg                                                                              <  7:44717/148‑1 (MQ=255)
                                                                   tCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtggg                                                                              <  4:21940/148‑1 (MQ=255)
                                                                     gcgATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCaa                                                                                                                                        <  8:352234/88‑1 (MQ=255)
                                                                     gcgATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCaa                                                                                                                                        >  7:352234/1‑88 (MQ=255)
                                                                           tgactgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCACACAAAAACGCAGGCTAAGCTACCCAATCATGCGGCAAAAAAATGGAAGCGGCATACGGCTGGGGATGTGGAt                                                                     >  3:44575/1‑149 (MQ=255)
                                                                             actgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAttt                                                                   <  4:80409/149‑1 (MQ=255)
                                                                             actgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGGTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGa                                                                      <  6:134577/146‑1 (MQ=255)
                                                                                      ggCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGTATCGCCCAGACCAAAACGCTGGCTAAGGTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATc                                                           <  5:53141/148‑1 (MQ=255)
                                                                                      ggCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCAAAGCTTGCCAATAATGCGGCAAAAAAATGGCATCGGCAGACGGGTGGGTTGGTGGATTTATCAAAtat                                                          >  6:280627/1‑147 (MQ=255)
                                                                                       gCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCt                                                          <  6:136041/148‑1 (MQ=255)
                                                                                        cAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTg                                                         >  2:403057/1‑148 (MQ=255)
                                                                                         agagAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGggtggt                                                                         >  2:75700/1‑131 (MQ=255)
                                                                                         agagAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGggtggt                                                                         <  1:75700/131‑1 (MQ=255)
                                                                                          gagaAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGaa                                                      >  3:10828/1‑149 (MQ=255)
                                                                                               aTTCGCGCAACGGTGCTACATGGTACGCATCTTAATGTTTGTTTGGGGATCGCACAGACCAAATCGCGTGCTATGATTGCCATTCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTTGTGGATTTATGATATCTGGAACGCCa                                                 <  4:44575/149‑1 (MQ=255)
                                                                                               aTTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGCCCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGc                                                   >  8:336709/1‑147 (MQ=255)
                                                                                                     gcAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTa                                            >  2:50471/1‑148 (MQ=255)
                                                                                                        aCGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGAAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAAt                                         >  1:294980/1‑148 (MQ=255)
                                                                                                          ggTGCTACCACGTATCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTaa                                      <  5:183375/149‑1 (MQ=255)
                                                                                                           gTGCTACAACGAACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAAt                                     <  3:9531/149‑1 (MQ=255)
                                                                                                            tGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATg                                    >  6:403056/1‑149 (MQ=255)
                                                                                                                           aTCTTACAGTTGGTCTGGGGATATACCACACCAAACCTCTGGCTAAGCTTGAAAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGCGAGGTGTATTTATCAAATCTCTAACGCCAGCGTAAATTAATTTATGCTCACCCAGTg                     >  2:279478/1‑149 (MQ=255)
                                                                                                                            tcataatGTTGGTGTGGCGATCGCCCAGACCAAAACGCAGGCTAAGCTTGCCAATCATTCGGCAAAAAAATGGCAGCGGCAGACGGGTGTGGTGGTGGATTTATCAAATCTGTAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTgg                    <  2:294980/143‑1 (MQ=255)
                                                                                                                            tCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTg                     >  7:71572/1‑148 (MQ=255)
                                                                                                                            tCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTg                     >  7:54113/1‑148 (MQ=255)
                                                                                                                                aCTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGa                   <  7:353210/146‑1 (MQ=255)
                                                                                                                                 cTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGa                <  8:71572/148‑1 (MQ=255)
                                                                                                                                    ttGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTc            <  7:318620/149‑1 (MQ=255)
                                                                                                                                    ttGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTc            >  7:64604/1‑149 (MQ=255)
                                                                                                                                        tgtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAtt                                                                    <  2:40264/89‑1 (MQ=255)
                                                                                                                                        tgtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAtt                                                                    >  1:40264/1‑89 (MQ=255)
                                                                                                                                         gtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAACATGGCAGCGGCAGCCGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCGCCGTGGAGGCCGTCTgggg       >  4:145798/1‑149 (MQ=255)
                                                                                                                                         gtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTgggg       >  1:401229/1‑149 (MQ=255)
                                                                                                                                          tgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCAGTGGATGACGTCTgggg       >  8:98410/1‑148 (MQ=255)
                                                                                                                                          tgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTATGCTCTCCCCGTGGATGACGTCTgggg       <  3:140273/148‑1 (MQ=255)
                                                                                                                                          tgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAAGGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTgggg       >  1:110934/1‑148 (MQ=255)
                                                                                                                                               gATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTg  >  8:390836/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTG  >  NC_000913/1231457‑1231747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: