Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,490,628 A→G 5.4% M171T (ATG→ACG)  idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,6280AG 5.4% 230.6 / 2.8 74M171T (ATG→ACG) idnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base A (38/32);  new base G (2/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATGGTGTGATATTTTTCCGAAAGT  >  NC_000913/4490481‑4490768
                                                                                                                                                   |                                                                                                                                            
gcgcGTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCa                                                                                                                                              <  3:148069/148‑1 (MQ=255)
 cgcgTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATg                                                                                                                                            <  1:250275/149‑1 (MQ=255)
          tCATGCGTAATGGGGAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCACTTCTCCAGCATGGTACACACCATGTCAAAAGCg                                                                                                                                   >  5:38248/1‑149 (MQ=255)
           cATGCGTAATGGGGAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCg                                                                                                                                   <  3:78716/148‑1 (MQ=255)
            aTGCGTAATGGGGAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGc                                                                                                                                 >  4:134773/1‑149 (MQ=255)
                  aaTGGGGAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGATGTTCATCTCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTCACTCGCTTCTCCAGCATGGAACACACCATGTCAAAAGCGGCCTGcc                                                                                                                            >  2:66864/1‑148 (MQ=255)
                   aTGGGGAAAGATTATGCAGCATCATCGCATCGAAATACCCGTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCTTGGTACACACCATGTCAAAAGCGGCCTGCCGa                                                                                                                          <  1:66864/149‑1 (MQ=255)
                     gctgAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGAtt                                                                                                                        <  5:204897/146‑1 (MQ=255)
                      gggAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTa                                                                                                                       >  6:277585/1‑149 (MQ=255)
                        gAAAGATTATGCAGCATCATCGCATCTCAAGAACCCTGATAACGCTGTTCATCGCGGGTTTCATCTTTGTAACCGATATACAGAAATTTGTCCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATc                                                                                                                     <  2:304470/149‑1 (MQ=255)
                        gAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGAtt                                                                                                                        >  1:216632/1‑146 (MQ=255)
                        gAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGAtt                                                                                                                        <  2:216632/146‑1 (MQ=255)
                        gAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATc                                                                                                                     <  4:369600/149‑1 (MQ=255)
                        gAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATc                                                                                                                     <  2:309961/149‑1 (MQ=255)
                        gAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCACTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATc                                                                                                                     <  6:38248/149‑1 (MQ=255)
                         aaaGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTCCACACCATGTCAAAAGCGGCCTGCCGAtt                                                                                                                        >  6:204897/1‑145 (MQ=255)
                         aaaGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATc                                                                                                                     >  5:143437/1‑148 (MQ=255)
                         aaaGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATc                                                                                                                     <  6:143437/148‑1 (MQ=255)
                         aaaGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCa                                                                                                                    <  3:269017/149‑1 (MQ=255)
                                 tGCAGCATCATCGCATCGCAATAACCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGaa                                                                                                            <  5:277585/149‑2 (MQ=255)
                                    agcaTCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTc                                                                                                         >  7:44930/1‑149 (MQ=255)
                                     gcaTCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTcc                                                                                                        <  3:134773/149‑1 (MQ=255)
                                     gcaTCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCCGACTCGCTTCTCCAGCATGGTACACACCGTGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTc                                                                                                         >  1:154854/1‑148 (MQ=255)
                                        tcatcGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCata                                                                                                     >  3:316654/1‑149 (MQ=255)
                                         catcgcatcgCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCatat                                                                                                    >  2:55500/1‑149 (MQ=255)
                                             gcatcgcaATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAg                                                                                                >  5:188141/1‑149 (MQ=255)
                                               atcgcaATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGcc                                                                                              <  7:87449/149‑1 (MQ=255)
                                                   caATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTtc                                                                                          <  1:55500/149‑1 (MQ=255)
                                                     aTACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATc                                                                                                                     >  3:203305/1‑120 (MQ=255)
                                                     aTACCCCTGATAACGCTGGTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATc                                                                                                                     <  4:203305/120‑1 (MQ=255)
                                                       aCCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTccc                                                                                      >  3:341733/1‑149 (MQ=255)
                                                        ccccTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCt                                                                                     <  5:265245/149‑1 (MQ=255)
                                                         cccTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTccc                                                                                      >  3:47880/1‑147 (MQ=255)
                                                         cccTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTg                                                                                    >  6:214305/1‑149 (MQ=255)
                                                         ccatGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTccc                                                                                      <  4:47880/144‑1 (MQ=255)
                                                            tGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGCCCTCCATATCCAGCCGTTCTCCCTGTa                                                                                  >  1:204475/1‑148 (MQ=255)
                                                            tGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACAATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTa                                                                                  >  2:290359/1‑148 (MQ=255)
                                                                  cGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTAcatccat                                                                           >  5:118249/1‑149 (MQ=255)
                                                                   gCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGt                                                                                                                                           >  2:131406/1‑84 (MQ=255)
                                                                   gCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGt                                                                                                                                           <  1:131406/84‑1 (MQ=255)
                                                                         cATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTc                                                                     <  8:44930/148‑1 (MQ=255)
                                                                                 gtgtCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCg                                                             >  2:175285/1‑148 (MQ=255)
                                                                                   gtCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGaa                                                          >  5:182335/1‑149 (MQ=255)
                                                                                    tCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGAc                                                                                                            >  8:172773/1‑98 (MQ=255)
                                                                                    tCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGaa                                                          <  3:293565/148‑1 (MQ=255)
                                                                                           tGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGc                                                   <  1:175285/148‑1 (MQ=255)
                                                                                              aaCCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGaa                                               <  4:341733/149‑1 (MQ=255)
                                                                                              aaCCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGaa                                               <  1:290359/149‑1 (MQ=255)
                                                                                                       aCAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAAt                                      >  8:112177/1‑149 (MQ=255)
                                                                                                         aGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGaaa                                       >  3:253319/1‑146 (MQ=255)
                                                                                                         aGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGaaa                                       <  4:253319/146‑1 (MQ=255)
                                                                                                         aGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAAttt                                    >  8:327688/1‑149 (MQ=255)
                                                                                                           aaTTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCa                                  >  7:24562/1‑149 (MQ=255)
                                                                                                            aTTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCAc                                 <  6:182335/149‑1 (MQ=255)
                                                                                                                   gCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCt                           >  1:190180/1‑148 (MQ=255)
                                                                                                                    cccgACTCGCTTCTCCAGCATGGTACACACCGTGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGa                         <  2:154854/146‑1 (MQ=255)
                                                                                                                    ccTGACTCGCTTCTCCAGCATGGTACACACCGTGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGa                         >  2:85473/1‑149 (MQ=255)
                                                                                                                    ccTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGa                         >  8:391441/1‑149 (MQ=255)
                                                                                                                    ccTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATACAGCCGTTCACCCAGTACATCCATCAATTCACAGACCGGAATGGTGGCTGAAAGAAGAAATTTAACCGTCCTGa                         >  8:391445/1‑149 (MQ=255)
                                                                                                                     cTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGCCCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGAt                        >  7:135395/1‑149 (MQ=255)
                                                                                                                      tGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGAACATCCATCAATACCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATg                       >  7:388921/1‑149 (MQ=255)
                                                                                                                        aCTCGCTTCTCCAGCATGGTACACACCGTGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATgg                      <  1:85473/148‑1 (MQ=255)
                                                                                                                        aCTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATGgt                     >  3:265100/1‑149 (MQ=255)
                                                                                                                         cTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTTCATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATGgtg                    <  4:316654/149‑1 (MQ=255)
                                                                                                                         cTCGCTTCTCCAGCATGGTACACACCATGACAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAAATCCACGACCGGAATTGTGGCTGAACGCAGAAATTTCACCGTCCTGATGgtg                    >  4:147505/1‑149 (MQ=255)
                                                                                                                          tCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTc                                   >  6:17435/1‑133 (MQ=255)
                                                                                                                          tCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTc                                   <  5:17435/133‑1 (MQ=255)
                                                                                                                            gCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATGGtgtg                  <  5:214305/148‑1 (MQ=255)
                                                                                                                            gCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATGGTGTGa                 >  8:216799/1‑149 (MQ=255)
                                                                                                                            gCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAAGGGTGGCTGAACGCAGAAATTTCACCGTCCTGATGGTGTGa                 >  6:253355/1‑149 (MQ=255)
                                                                                                                                   cAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATGGTGTGATAttttt          >  4:153669/1‑149 (MQ=255)
                                                                                                                                     gCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCTCGACCGGAATGGTGGCTGAACGCAGAAATTTCAACGTCCTGATGGTGTGATATTTTTcc        <  4:265100/149‑1 (MQ=255)
                                                                                                                                           tACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATGGTGTGATATTTTTCCGAAAGt  <  6:188141/149‑1 (MQ=255)
                                                                                                                                                  cATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAAtggtg                                                     <  7:293197/91‑1 (MQ=255)
                                                                                                                                                  cATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAAtggtg                                                     >  8:293197/1‑91 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GCGCGTGGATTCATGCGTAATGGGGAAAGATTATGCAGCATCATCGCATCGCAATACCCCTGATAACGCTGTTCATCGCGGGTGTCATCTTTGGAACCGAGATACAGAATTTTGTGCCTGACTCGCTTCTCCAGCATGGTACACACCATGTCAAAAGCGGCCTGCCGATTATCAAAACCGACCTCCATATCCAGCCGTTCTCCCTGTACATCCATCAATTCCACGACCGGAATGGTGGCTGAACGCAGAAATTTCACCGTCCTGATGGTGTGATATTTTTCCGAAAGT  >  NC_000913/4490481‑4490768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: