Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 892,020 G→T 5.2% L18L (CTG→CTT rimK → ribosomal protein S6 modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913892,0200GT 5.2% 297.5 / 2.6 86L18L (CTG→CTTrimKribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (44/37);  new base T (3/2);  total (47/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCA  >  NC_000913/891872‑892165
                                                                                                                                                    |                                                                                                                                                 
attattTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTg                                                                                                                                                   <  7:376968/149‑1 (MQ=255)
attattTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGAGGTATGATACGCGGGCTTTTGACAAGGTCTGACAGAGAGGGGCAGGGTGAAAATTGCCAGATTGTGCCGGGATGGACCGCGCTATTCGTGTAAGCGGCTg                                                                                                                                                   >  2:319802/1‑149 (MQ=255)
    tttGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACTGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGt                                                                                                                                                <  3:171302/148‑1 (MQ=255)
       gCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGaa                                                                                                                                             <  4:342993/148‑1 (MQ=255)
             aCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCta                                                                                                                                      <  7:224743/149‑1 (MQ=255)
             aCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCta                                                                                                                                      >  2:19528/1‑149 (MQ=255)
                     gggCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCAGCAATACAgcgc                                                                                                                              >  3:385420/1‑149 (MQ=255)
                         gACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTGGACCAGGTCTGACAGAGAGGTGCAGGGTGAAACTTGCCATATTGTCCCGGGATGGACCGCGCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCgggc                                                                                                                          <  7:287304/149‑3 (MQ=255)
                              gcTTAAACCGCCTCGTAGATGGATGATACGCGGGCGTTTGACCAGGTCTGAGCGAGAGGTGTAGGGGGAAAATTGCCATATTGTCCCGGGCGGTAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATTCAGCGCGGTCAGCTg                                                                                                                     <  6:72426/149‑1 (MQ=255)
                              gcTAAAACCGCCACGTCGATGTATGCTACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTg                                                                                                                     >  1:88654/1‑149 (MQ=255)
                              gcTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTg                                                                                                                     >  7:151406/1‑149 (MQ=255)
                                  aaaCCGCCATGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTg                                                                                                                 <  1:417104/149‑1 (MQ=255)
                                   aaCCGCCAAGTCGATGTTTGTTACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTAGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGa                                                                                                                <  4:385420/149‑1 (MQ=255)
                                      cGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAAt                                                                                                             <  2:307446/149‑1 (MQ=255)
                                       gCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAAtt                                                                                                            >  1:187670/1‑149 (MQ=255)
                                        ccACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTc                                                                                                           <  2:187670/149‑1 (MQ=255)
                                         cACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCt                                                                                                          <  3:274265/149‑1 (MQ=255)
                                         cACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCt                                                                                                          <  8:277765/149‑1 (MQ=255)
                                          aCGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCtt                                                                                                         <  3:222861/149‑1 (MQ=255)
                                            gTCGATGTATGATACGCGGGCTTTTGCCCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCtt                                                                                                         >  3:26224/1‑147 (MQ=255)
                                             tCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGAt                                                                                                      <  8:256478/149‑1 (MQ=255)
                                              cGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATc                                                                                                     <  7:176089/149‑1 (MQ=255)
                                                    aTGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCttt                                                                                               >  5:120769/1‑149 (MQ=255)
                                                     tGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTc                                                                                              <  5:376721/149‑1 (MQ=255)
                                                     tGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTc                                                                                              >  8:131232/1‑149 (MQ=255)
                                                       aTACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCtt                                                                                            <  7:131232/149‑1 (MQ=255)
                                                       aTACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCt                                                                                             <  6:180036/148‑1 (MQ=255)
                                                         aCGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGc                                                                                          >  4:231622/1‑149 (MQ=255)
                                                           ccgGGCTTTTGACCATGTCTGACAGCGAGGTGCAGGGTGAAAATTGCCATTTTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTa                                                                                        <  2:88654/148‑1 (MQ=255)
                                                               gCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCTGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGATACAt                                                                                     >  6:235030/1‑148 (MQ=255)
                                                                 ttttGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGaa                                                                                  >  3:351321/1‑149 (MQ=255)
                                                                   ttGACAAGGTCTTTCAGATAGGTTAAGGGTCAAAATTGACATATTGTCCCGGGATGGTAGGCTCTATTCGTGTAAGCGTCTGCGTGAAGCCGCTATACATCTCTGTCACATGTTTGAAATGCTTTATCCGCTTTCTTGCTACATGaaa                                                                                 <  4:222970/148‑2 (MQ=255)
                                                                    tGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACa                                                                                <  8:151406/148‑1 (MQ=255)
                                                                      aCCAGGTCTGACAGAGAGGTGCTGGGGGAAAATTGCCATATTGTCCCGGGATGGAACGGGCTATTCGTGTAAGGGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATaa                                                                             <  6:316880/149‑1 (MQ=255)
                                                                      aCCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCAGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATaa                                                                             <  4:231300/149‑1 (MQ=255)
                                                                         aGGTCTGACAGAGAGGTGCATGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGCCTGAGTGAAGCCGCTATACAGCGCGGTCACATGGTTGAAATTGTTGATCCGCTTTCTTGCTACATGAACATAAATc                                                                          >  2:67051/1‑149 (MQ=255)
                                                                         aGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATc                                                                          >  2:261339/1‑149 (MQ=255)
                                                                           gTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCt                                                                        >  5:284212/1‑149 (MQ=255)
                                                                              tGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGtt                                                                                                                  >  2:149973/1‑104 (MQ=255)
                                                                                aCAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTgcg                                                                     <  7:205899/147‑1 (MQ=255)
                                                                                            aGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGGCTTCTATTCa                                                       <  5:52029/149‑1 (MQ=255)
                                                                                             gggTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCAc                                                      >  1:257399/1‑149 (MQ=255)
                                                                                               gTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGCAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTa                                                    >  1:30526/1‑149 (MQ=255)
                                                                                               gTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCCCCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGGCTTCTATTCACTa                                                    >  8:186687/1‑149 (MQ=255)
                                                                                                tGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTa                                                    >  5:245193/1‑148 (MQ=255)
                                                                                                tGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTa                                                    >  2:224966/1‑148 (MQ=255)
                                                                                                      ttGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGATTCGTGAAGCCGCTATACTGGGCGGTCACCTGGTTGAAATTCTTGTTCCGCTTTCTTGCTACATGAACATTAATCCTGCGGCGTCTTCTATTCACTACAAAGGc                                             <  8:296960/149‑1 (MQ=255)
                                                                                                        gCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGcc                                            <  6:120769/148‑1 (MQ=255)
                                                                                                        gCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAACGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGcc                                            <  6:229226/148‑1 (MQ=255)
                                                                                                            tatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCa                                                       <  7:121752/133‑1 (MQ=255)
                                                                                                            tatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCa                                                       >  8:121752/1‑133 (MQ=255)
                                                                                                            tatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAg                                       >  3:368972/1‑149 (MQ=255)
                                                                                                            tatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAg                                       >  4:343006/1‑149 (MQ=255)
                                                                                                            tatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAg                                       >  4:35973/1‑149 (MQ=255)
                                                                                                            tatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAg                                       >  1:243597/1‑149 (MQ=255)
                                                                                                             atTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGt                                      >  5:354872/1‑149 (MQ=255)
                                                                                                                   ccGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTAccc                                 >  8:9890/1‑148 (MQ=255)
                                                                                                                     gggATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCa                                                       >  4:162140/1‑124 (MQ=255)
                                                                                                                     gggATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCa                                                       <  3:162140/124‑1 (MQ=255)
                                                                                                                     gggATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCAt                              >  5:354108/1‑149 (MQ=255)
                                                                                                                        aTGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCAtttt                           <  3:343006/149‑1 (MQ=255)
                                                                                                                         tGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCaa                                        >  4:139726/1‑135 (MQ=255)
                                                                                                                         tGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCaa                                        <  3:139726/135‑1 (MQ=255)
                                                                                                                         tGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCAtttt                           >  2:10261/1‑148 (MQ=255)
                                                                                                                         tGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCAtttt                           >  2:285612/1‑148 (MQ=255)
                                                                                                                           gAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGAc                        >  3:359569/1‑149 (MQ=255)
                                                                                                                            aaCGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACg                       >  7:417752/1‑149 (MQ=255)
                                                                                                                            aaCGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACg                       >  2:393350/1‑149 (MQ=255)
                                                                                                                                ctctATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGt                   >  2:190855/1‑149 (MQ=255)
                                                                                                                                 tctATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAg                    >  1:353170/1‑147 (MQ=255)
                                                                                                                                 tctATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAg                    <  2:353170/147‑1 (MQ=255)
                                                                                                                                 tctATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGt                   >  1:326076/1‑148 (MQ=255)
                                                                                                                                   tATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGAt                >  6:99042/1‑149 (MQ=255)
                                                                                                                                   tATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGAt                >  4:25346/1‑149 (MQ=255)
                                                                                                                                   tATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGAt                >  5:386249/1‑149 (MQ=255)
                                                                                                                                         tgtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCcgcg          <  6:354872/149‑1 (MQ=255)
                                                                                                                                          tataGCGGCTTCACGCAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCcgcg          >  1:292774/4‑148 (MQ=255)
                                                                                                                                           tAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTa        <  1:10261/149‑1 (MQ=255)
                                                                                                                                            taGCGGCTTCACGCAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTa        >  1:85229/2‑148 (MQ=255)
                                                                                                                                            taGCGGCTTCACGCAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTa        <  2:85229/147‑1 (MQ=255)
                                                                                                                                            taGCGGCTTCACGCAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTAt       >  8:39958/2‑149 (MQ=255)
                                                                                                                                            aaGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATACGCTTTCTTGCTACATGAACATAAATCCTGCGGCGGCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTa        <  1:165170/148‑1 (MQ=255)
                                                                                                                                             aGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTAtt      <  2:326076/149‑1 (MQ=255)
                                                                                                                                             aGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTAtt      >  8:387970/1‑149 (MQ=255)
                                                                                                                                              gCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGtcttc                                                             <  2:212406/93‑1 (MQ=255)
                                                                                                                                              gCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGtcttc                                                             >  1:212406/1‑93 (MQ=255)
                                                                                                                                              gCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTg     >  7:114501/1‑149 (MQ=255)
                                                                                                                                                 gCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCa  <  1:285612/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCA  >  NC_000913/891872‑892165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: