| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,231,594 | G→C | 7.3% | V137L (GTG→CTG) | umuC → | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,231,594 | 0 | G | C | 7.3% | 261.8 / 9.1 | 79 | V137L (GTG→CTG) | umuC | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity |
| Reads supporting (aligned to +/- strand): ref base G (32/40); new base C (3/3); total (35/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
TGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGG > NC_000913/1231448‑1231742 | tGGAAGAGCTATCGCCCCGCGTCGAGAATTCCAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCTGAATTGTCGCGATCTGACTGATTTTGTCAGAGAAATTCGAGAAACTGTGCTAGATCGTACCCATCATACTTTTGTtgtg > 1:280276/1‑149 (MQ=255) gAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTggg < 7:279736/149‑1 (MQ=255) gAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTggg > 7:219964/1‑149 (MQ=255) gAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTgg < 5:192872/148‑1 (MQ=255) gagCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GAt < 1:46389/149‑1 (MQ=255) gagCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GAt > 2:46389/1‑149 (MQ=255) tATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGcc > 7:214804/1‑149 (MQ=255) aTCGCCACGCGACGAGATTTACACTATTGATGAGCCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTCGTGTGGG‑GATCGcc > 2:403380/1‑148 (MQ=255) tCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGcc > 3:80409/1‑147 (MQ=255) gcGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTTCGATCTGCCAGGGGGGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACACCGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCa < 8:80841/148‑1 (MQ=255) ccccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGAc > 5:136041/1‑149 (MQ=255) cccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑TATCGCCCAGAc > 6:53141/1‑148 (MQ=255) ccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGgtgt > 1:84784/1‑132 (MQ=255) ccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGgtgt < 2:84784/132‑1 (MQ=255) ccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGAcc > 5:76340/1‑148 (MQ=255) cgTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATC‑CCCACACCaaca < 3:171118/149‑3 (MQ=255) tCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACg > 3:87088/1‑149 (MQ=255) agaTTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTg < 7:95693/149‑1 (MQ=255) gaTTTACAGTATTGATGAGGCTTTCTGCGATCTGACAGGTGTGCGTTATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTg < 6:365762/148‑1 (MQ=255) tACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATC‑CCCACACCaaca > 8:109343/1‑137 (MQ=255) tACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATC‑CCCACACCaaca < 7:109343/139‑3 (MQ=255) aCAGTATTGATGCGGCATTCTGCGATCTGACAGGGGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTaa < 1:273691/148‑1 (MQ=255) gTATTGATGAGGCATTCTGCGAGCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCtt < 8:212420/149‑1 (MQ=255) aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa < 2:250418/148‑1 (MQ=255) aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa < 8:182337/148‑1 (MQ=255) aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa < 6:76340/148‑1 (MQ=255) ggCATTCTGCGATCTGACATGTGTGCGTAATTGTCGCGATCTGACTGATTTTGTCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATg < 8:1206/149‑1 (MQ=255) ggCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATC‑CCCACACCaacag > 3:55894/1‑123 (MQ=255) ggCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATC‑CCCACACCaacag < 4:55894/126‑4 (MQ=255) gCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACATCGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGc < 2:346421/149‑1 (MQ=255) gCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGc > 3:21940/1‑149 (MQ=255) cATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACTGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCg < 1:253696/149‑1 (MQ=255) ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATc < 3:59393/142‑1 (MQ=255) ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATc > 4:59393/1‑142 (MQ=255) ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa > 4:81772/1‑143 (MQ=255) ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa < 3:81772/143‑1 (MQ=255) cTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCa > 2:271203/1‑148 (MQ=255) tGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaa > 6:207271/1‑148 (MQ=255) aTCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa > 2:300577/1‑149 (MQ=255) aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa < 8:339729/144‑1 (MQ=255) aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa < 3:266965/144‑1 (MQ=255) aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAAt < 6:323158/145‑1 (MQ=255) aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATg < 5:207271/146‑1 (MQ=255) aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCa < 1:300577/149‑1 (MQ=255) cAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAg < 1:403380/149‑1 (MQ=255) aGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGc < 8:219964/149‑1 (MQ=255) tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa > 6:344899/1‑124 (MQ=255) tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa < 5:344899/124‑1 (MQ=255) tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAg > 5:271007/1‑149 (MQ=255) cGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGAc > 8:318620/1‑149 (MQ=255) tAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgg > 8:353210/1‑149 (MQ=255) aTTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggt < 8:214804/149‑1 (MQ=255) ttGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtg < 2:189970/149‑1 (MQ=255) tCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtggg < 4:21940/148‑1 (MQ=255) tCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtggg < 7:44717/148‑1 (MQ=255) gcgATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCaa < 8:352234/88‑1 (MQ=255) gcgATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCaa > 7:352234/1‑88 (MQ=255) tgactgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCACACAAAAACGCAGGCTAAGCTACCCAATCATGCGGCAAAAAAATGGAAGCGGCATACGGCTGGGGATGTGGAt > 3:44575/1‑149 (MQ=255) actgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAttt < 4:80409/149‑1 (MQ=255) actgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGGTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGa < 6:134577/146‑1 (MQ=255) ggCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑TATCGCCCAGACCAAAACGCTGGCTAAGGTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATc < 5:53141/148‑1 (MQ=255) ggCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCAAAGCTTGCCAATAATGCGGCAAAAAAATGGCATCGGCAGACGGGTGGGTTGGTGGATTTATCAAAtat > 6:280627/1‑147 (MQ=255) gCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCt < 6:136041/148‑1 (MQ=255) cAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTg > 2:403057/1‑148 (MQ=255) agagAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGggtggt > 2:75700/1‑131 (MQ=255) agagAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGggtggt < 1:75700/131‑1 (MQ=255) gagaAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGaa > 3:10828/1‑149 (MQ=255) aTTCGCGCAACGGTGCTACATGGTACGCATCTTAATGTTTGTTTGGG‑GATCGCACAGACCAAATCGCGTGCTATGATTGCCATTCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTTGTGGATTTATGATATCTGGAACGCCa < 4:44575/149‑1 (MQ=255) aTTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGCCCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGc > 8:336709/1‑147 (MQ=255) gcAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTa > 2:50471/1‑148 (MQ=255) aCGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGAAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAAt > 1:294980/1‑148 (MQ=255) ggTGCTACCACGTATCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTaa < 5:183375/149‑1 (MQ=255) gTGCTACAACGAACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAAt < 3:9531/149‑1 (MQ=255) tGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATg > 6:403056/1‑149 (MQ=255) aTCTTACAGTTGGTCTGGG‑GATATACCACACCAAACCTCTGGCTAAGCTTGAAAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGCGAGGTGTATTTATCAAATCTCTAACGCCAGCGTAAATTAATTTATGCTCACCCAGTg > 2:279478/1‑149 (MQ=255) tcataatGTTGGTGTGGC‑GATCGCCCAGACCAAAACGCAGGCTAAGCTTGCCAATCATTCGGCAAAAAAATGGCAGCGGCAGACGGGTGTGGTGGTGGATTTATCAAATCTGTAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTgg < 2:294980/143‑1 (MQ=255) tCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTg > 7:71572/1‑148 (MQ=255) tCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTg > 7:54113/1‑148 (MQ=255) aCTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGa < 7:353210/146‑1 (MQ=255) cTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGa < 8:71572/148‑1 (MQ=255) ttGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTc > 7:64604/1‑149 (MQ=255) ttGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTc < 7:318620/149‑1 (MQ=255) tgtgGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAtt < 2:40264/89‑1 (MQ=255) tgtgGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAtt > 1:40264/1‑89 (MQ=255) gtgGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAACATGGCAGCGGCAGCCGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCGCCGTGGAGGCCGTCTgggg > 4:145798/1‑149 (MQ=255) gtgGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTgggg > 1:401229/1‑149 (MQ=255) | TGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG‑GATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGG > NC_000913/1231448‑1231742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |