BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	192,579	A→T	5.3%	*242L (TAA→TTA)	pyrH →	uridylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	192,579	0	A	T	5.3%	356.6 / 6.0	111	*242L (TAA→TTA)	pyrH	uridylate kinase
Reads supporting (aligned to +/- strand): ref base A (48/57); new base T (4/2); total (52/59)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAGCA  >  NC_000913/192432‑192727

tGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGaataa                                                                                                                                                     >  4:311964/1‑149 (MQ=255)

     aaaGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTccc                                                                                                                                                <  7:409516/149‑1 (MQ=255)

           cTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATg                                                                                                                                          >  7:31279/1‑149 (MQ=255)

             gAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGa                                                                                                                                        >  6:383762/1‑149 (MQ=255)

             gAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTATTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATTATTCCCGTGATGGa                                                                                                                                        >  1:36670/1‑149 (MQ=255)

                aGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataa                                                                                                                                     <  7:354165/149‑1 (MQ=255)

                  tCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaat                                                                                                                                   <  3:178601/149‑1 (MQ=255)

                    aTGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaata                                                                                                                                  <  6:353532/148‑1 (MQ=255)

                     tGGACCTGGCGGCCTTCACGCTGGCTCGTGAACATAAATTACCTATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGACGTGTTGTAATTGGTGAAAAAGAAGGTACTTTAATCAC‑GTAATAATTAACGTGATGGATAAATAAg                                                                                                                                >  2:69070/1‑149 (MQ=255)

                       gACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAgg                                                                                                                               <  2:284314/148‑1 (MQ=255)

                        aCCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAgg                                                                                                                               >  7:268799/1‑147 (MQ=255)

                        aCCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAgg                                                                                                                               <  8:268799/147‑1 (MQ=255)

                          cTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGt                                                                                                                             >  1:108778/1‑147 (MQ=255)

                           tGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGATAAAGAAGGGACTTTAATCAC‑GCAATAATTCCCGTGATGGATAAATAAggga                                                                                                                             >  7:289665/1‑145 (MQ=255)

                           tGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAg                                                                                                                                >  2:93257/1‑143 (MQ=255)

                           tGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAg                                                                                                                                <  1:93257/143‑1 (MQ=255)

                                 ccTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCcg                                                                                                                    >  1:180328/1‑149 (MQ=255)

                                  cTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCcgc                                                                                                                   >  1:183518/1‑149 (MQ=255)

                                  cTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCcgc                                                                                                                   >  3:60176/1‑149 (MQ=255)

                                   ttCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCcgcg                                                                                                                  >  8:315116/1‑149 (MQ=255)

                                   ttCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCCTGAGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCcgcg                                                                                                                  >  5:374289/1‑149 (MQ=255)

                                    tCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCcgcg                                                                                                                  >  5:307920/1‑148 (MQ=255)

                                        gCTGGCTCTTGACCATAAAGTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTaa                                                                                                               <  2:36670/147‑1 (MQ=255)

                                        gCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGt                                                                                                             <  3:354657/149‑1 (MQ=255)

                                          tGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTAt                                                                                                           >  5:102851/1‑149 (MQ=255)

                                             cTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATcgc                                                                                                        <  7:145502/149‑1 (MQ=255)

                                                gTGACCATAAATTACCGAGTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCgg                                                                                                      <  5:13719/148‑1 (MQ=255)

                                                 tGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCgggg                                                                                                    <  3:13796/149‑1 (MQ=255)

                                                 tGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGAAATGGGTGAAAAAGAACGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCgggg                                                                                                    >  6:227499/1‑149 (MQ=255)

                                                  gACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCggggg                                                                                                   >  6:15323/1‑149 (MQ=255)

                                                      aTAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGt                                                                                                >  1:56800/1‑148 (MQ=255)

                                                       tAAATTACCGATTCGTTTTTTAAATATGAACAAAACGGGTGCGCTTCGCCGTGTGGTAATGGGTGAAAAAGAATGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTaa                                                                                              <  6:147558/149‑1 (MQ=255)

                                                       tAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGt                                                                                                >  7:276071/1‑147 (MQ=255)

                                                       tAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTaa                                                                                              <  2:108778/149‑1 (MQ=255)

                                                       tAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTaa                                                                                              <  1:363162/149‑1 (MQ=255)

                                                       tAAATTACCGATTCGTCTTTTAATTATGAACAAAGCGGGTGCGCTGCGCCGTGTGGTAATGGTTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTAGCGCGGGGGCGTaa                                                                                              <  8:289665/149‑1 (MQ=255)

                                                        aaaTTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAg                                                                                             >  1:184835/1‑149 (MQ=255)

                                                         aattcacGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGGGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GTAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGt                                                                                            <  1:347602/143‑1 (MQ=255)

                                                         aaTTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGt                                                                                            >  3:206965/1‑149 (MQ=255)

                                                           ttACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaata                                                                                                                                  <  4:307480/109‑1 (MQ=255)

                                                           ttACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaata                                                                                                                                  >  3:307480/1‑109 (MQ=255)

                                                           ttACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCt                                                                                          <  2:184835/149‑1 (MQ=255)

                                                           ttACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCt                                                                                          <  7:59573/149‑1 (MQ=255)

                                                           ttACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCt                                                                                          >  2:122092/1‑149 (MQ=255)

                                                            tagcGATTCGTGTTTTCAATATGAACTTACCGGGTGCGCTGCGCCGTGTGGTTATGGGTGAAAAAGATGGGTCTTAAATCAC‑GGAATAATTGCCGTGATTGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCt                                                                                          <  5:146022/145‑1 (MQ=255)

                                                             aCCGATTCGTCTTTTCTATATGACCAAAACGGGTTGGCTGCGCCGTGTGGTAATGGGTGAATAATAAGTGAATTTAATAAC‑GGAATAATTGCTGTGATTGATAAATAATTGTCAGATTCCGGGTAAGTATCGCGGGGGCGTAAGGCTg                                                                                         <  4:309855/148‑1 (MQ=255)

                                                              ccGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTgg                                                                                        <  6:307920/148‑1 (MQ=255)

                                                              ccGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTgg                                                                                        <  4:206965/148‑1 (MQ=255)

                                                              ccGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGt                                                                                       <  2:254122/149‑1 (MQ=255)

                                                              ccGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGt                                                                                       >  8:248068/1‑149 (MQ=255)

                                                               cGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGtt                                                                                      <  2:394715/149‑1 (MQ=255)

                                                                gATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTta                                                                                     <  2:337795/149‑1 (MQ=255)

                                                                    cGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATaa                                                                                  <  5:383762/148‑1 (MQ=255)

                                                                        ttttCAATATGAACAAAACGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGt                                                                             <  5:7166/149‑1 (MQ=255)

                                                                          ttCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTgg                                                                                        >  7:9523/1‑136 (MQ=255)

                                                                          ttCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTa                                                                           >  4:384083/1‑149 (MQ=255)

                                                                             aaTATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAtt                                                                         <  2:183518/148‑1 (MQ=255)

                                                                              atatGAACAAACCGTGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAGGGCGTTAttgt                                                                       <  1:69070/149‑1 (MQ=255)

                                                                               tatGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAttgtt                                                                      >  7:418825/1‑149 (MQ=255)

                                                                                      aaaCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcaggca                                                               <  4:160991/149‑1 (MQ=255)

                                                                                        aCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGt                                                             >  6:186006/1‑149 (MQ=255)

                                                                                          cGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGttt                                                           <  5:15323/149‑1 (MQ=255)

                                                                                           gggTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTg                                                          <  5:227499/149‑1 (MQ=255)

                                                                                              tgcgctgcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGt                                                        >  5:355500/1‑148 (MQ=255)

                                                                                              tgcgctgcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTc                                                       <  5:284125/149‑1 (MQ=255)

                                                                                              tgcgctgcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAAGTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGGAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGt                                                        >  1:216267/1‑148 (MQ=255)

                                                                                                cgctgcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCAc                                                     <  6:102851/149‑1 (MQ=255)

                                                                                                  ctgcgcCGTGTGGTAATGGGTGAAAAAGAAGAGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACgg                                                   <  7:74748/149‑1 (MQ=255)

                                                                                                   tgcgcCGTGTGGTATTGGGTTAAAAAGAAGGGTCTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAATGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAATGCGTTATTGTTGCAGGCAGTTTGGTCACGGc                                                  <  2:216267/149‑1 (MQ=255)

                                                                                                          tgtgGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAgcgcg                                           <  2:216376/149‑1 (MQ=255)

                                                                                                             ggTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCgcag                                        >  4:243273/1‑149 (MQ=255)

                                                                                                              gTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAgc                                              >  4:74712/1‑142 (MQ=255)

                                                                                                              gTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAgc                                              <  3:74712/142‑1 (MQ=255)

                                                                                                               tAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCCGCGCgcagc                                       >  4:71167/1‑148 (MQ=255)

                                                                                                                  tGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCGGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCTGTTTGGTCACGGCCAGCGCGCAGCAAcc                                   >  5:77294/1‑149 (MQ=255)

                                                                                                                  tGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATcgc                                                                                                        >  6:100825/1‑80 (MQ=255)

                                                                                                                  tGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAAcc                                   >  5:71561/1‑149 (MQ=255)

                                                                                                                      tGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAg                               <  3:243273/149‑1 (MQ=255)

                                                                                                                          aaaGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTTTTGTTGCAGGGAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGt                            <  6:374289/148‑1 (MQ=255)

                                                                                                                          aaaGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTa                           <  6:233836/149‑1 (MQ=255)

                                                                                                                               agGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACaaaa                      <  5:186006/149‑1 (MQ=255)

                                                                                                                                   acattAATCAC‑GGAATAATACCCGTGATGGATAAATAAGGGTAACATTACGCGAAAGTATCGCGGCGGCGTAAGTATGGATATAAGACGTTATTGTAGCAGGCAGATAGGACAAGGCCAGCGCGCAGCAACCGAAGCGAACAAAAGAAc                  >  2:174488/4‑149 (MQ=255)

                                                                                                                                     tttAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGGCACGGCCAGCGCGCAGCACCCGGAGCGTACAATAGTACGt                >  1:347093/1‑149 (MQ=255)

                                                                                                                                       tAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACaaaa                      >  6:90942/1‑141 (MQ=255)

                                                                                                                                       tAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACaaaa                      <  5:90942/141‑1 (MQ=255)

                                                                                                                                       tAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGt                <  3:384083/147‑1 (MQ=255)

                                                                                                                                       ccaTCACGGGAATTATT‑CCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGc                              >  3:14416/3‑133 (MQ=255)

                                                                                                                                       ccaTCACGGGAATTATT‑CCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGc                              <  4:14416/131‑1 (MQ=255)

                                                                                                                                       ccaTCACGGGAATTATT‑CCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGt                >  3:59905/3‑147 (MQ=255)

                                                                                                                                        aaTCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGa              <  8:31279/148‑1 (MQ=255)

                                                                                                                                        aaTCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAg             >  2:205767/1‑149 (MQ=255)

                                                                                                                                         aTCACGGGAATTATT‑CCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTg               >  3:267710/1‑146 (MQ=255)

                                                                                                                                          tCAC‑GGAATAATTCCCGTGATTGATAAATAAGGGTAAGATTCCGAGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGa           <  3:11536/149‑1 (MQ=255)

                                                                                                                                             c‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATgg        >  1:353257/1‑149 (MQ=255)

                                                                                                                                                 aataatTCCCGTGATGGATAAATAATGGTAAGATTCCGCGTAAGTATCGCTGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCTTCCGGAGCGTACAAAAGTACGTGAGGATGGCGa     <  1:174488/149‑1 (MQ=255)

                                                                                                                                                 aataatTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGa     <  3:374315/149‑1 (MQ=255)

                                                                                                                                                  ataatTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGtt                                                                            <  3:14841/77‑1 (MQ=255)

                                                                                                                                                  ataatTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGtt                                                                            >  4:14841/1‑77 (MQ=255)

                                                                                                                                                   taatTCCCGTGATGGATAAATAAGGGTACGAGTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAGc   <  1:308204/149‑1 (MQ=255)

                                                                                                                                                    aatTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAGCa  >  6:5572/1‑149 (MQ=255)

TGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAGCA  >  NC_000913/192432‑192727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`