Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,413,968 A→G 5.2% intergenic (‑41/+16) ralR ← / ← recT Rac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,9680AG 5.2% 357.8 / 6.9 115intergenic (‑41/+16)ralR/recTRac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein
Reads supporting (aligned to +/- strand):  ref base A (53/56);  new base G (2/4);  total (55/60)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 118 total aligned reads displayed.

GGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTT  >  NC_000913/1413822‑1414115
                                                                                                                                                  |                                                                                                                                                   
  gATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACAcc                                                                                                                                                 <  3:335572/149‑1 (MQ=255)
       gCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaa                                                                                                                                            <  5:258078/149‑2 (MQ=255)
       gCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCa                                                                                                                                             <  4:270330/148‑1 (MQ=255)
       gCATCCGTTACACTTCGCTCGGTAATATCCTGAAATCGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAAGTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaa                                                                                                                                            <  5:252255/149‑2 (MQ=255)
        cATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                                                                           <  2:359532/149‑3 (MQ=255)
          tCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCt                                                                                                                                         >  1:108196/1‑149 (MQ=255)
             gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                                                                           <  3:222518/144‑3 (MQ=255)
             gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                                                                           >  4:222518/1‑142 (MQ=255)
             gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGaa                                                                                                                                      >  2:332404/1‑149 (MQ=255)
             gTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGaa                                                                                                                                      >  3:323780/1‑149 (MQ=255)
                 cacTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAatta                                                                                                                                   <  1:221741/148‑1 (MQ=255)
                   cTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTc                                                                                                                                <  8:191662/149‑1 (MQ=255)
                    ttCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                                                                               >  2:126220/1‑149 (MQ=255)
                       gCTCGGTAATGTCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCctct                                                                                                                            <  1:289482/149‑1 (MQ=255)
                       gCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCctct                                                                                                                            <  6:386679/149‑1 (MQ=255)
                         tCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTg                                                                                                                           >  3:187274/1‑148 (MQ=255)
                         tCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGAAATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCAGTGCATTACACCGCCAGTCTGAATTATTCCTCTg                                                                                                                           >  7:267261/1‑148 (MQ=255)
                         tCGGTAATATCCGGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACAGTGTCATATCTCATAATTTTTCTCATAAAAAATATTTAAAGTTGGCGGGTCATTACACCGCCAGGCTGAATTATTCCTCTg                                                                                                                           >  8:236077/1‑148 (MQ=255)
                           ggTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAAt                                                                                                                        >  6:160004/1‑149 (MQ=255)
                           ggTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTAAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGaa                                                                                                                         <  1:42533/148‑1 (MQ=255)
                            gTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAAt                                                                                                                        >  7:109874/1‑148 (MQ=255)
                               atatCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATc                                                                                                                    <  6:98525/149‑1 (MQ=255)
                                  tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGa                                                                                                                  <  8:109874/148‑1 (MQ=255)
                                  tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGa                                                                                                                  <  2:108196/148‑1 (MQ=255)
                                  tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                 >  7:50017/1‑149 (MQ=255)
                                  tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                 >  7:162705/1‑149 (MQ=255)
                                  tCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTAATCCTCTGAATTATCGAt                                                                                                                 <  2:236003/149‑1 (MQ=255)
                                   ccTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTGAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                 >  2:87099/1‑148 (MQ=255)
                                    cTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                 <  2:381403/147‑1 (MQ=255)
                                    cTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                 >  1:381403/1‑147 (MQ=255)
                                    cTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTa                                                                                                               <  1:146394/149‑1 (MQ=255)
                                    cTGAAATGGCTTTACCCGTTACTGATTGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTa                                                                                                               <  7:414061/149‑1 (MQ=255)
                                       aaaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                                                                            >  5:187793/1‑149 (MQ=255)
                                       aaaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                                                                            >  6:390198/1‑149 (MQ=255)
                                        aaTGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt                                                                                                           <  4:112604/149‑1 (MQ=255)
                                           ggCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATGTCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTa                                                                                                        >  4:227311/1‑149 (MQ=255)
                                              tttCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTc                                                                                                     >  4:229261/1‑149 (MQ=255)
                                                 cACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTccc                                                                                                   <  4:156112/148‑1 (MQ=255)
                                                  aCCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACCCCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCg                                                                                                 >  8:133402/1‑149 (MQ=255)
                                                    cGTTACTGATGGAATACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGt                                                                                               <  4:206039/149‑1 (MQ=255)
                                                         cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATa                                                                                          <  3:350055/149‑1 (MQ=255)
                                                         cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATa                                                                                          <  4:327489/149‑1 (MQ=255)
                                                          tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCg                                                                                                                   <  5:68872/123‑1 (MQ=255)
                                                          tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCg                                                                                                                   >  6:68872/1‑123 (MQ=255)
                                                            aTGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACag                                                                                       <  6:295213/149‑1 (MQ=255)
                                                             tGGACAACCAGAAAATGGACATGGTTTAACATTGTCATATCTTATAATTTTTCTCATATAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGATTTATCGATTACACTTTATTCCCCGGTTAATACaga                                                                                      <  8:267261/149‑1 (MQ=255)
                                                             tGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACaga                                                                                      <  3:229261/149‑1 (MQ=255)
                                                             tGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACaga                                                                                      <  8:162705/149‑1 (MQ=255)
                                                               gACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACagag                                                                                     <  8:340727/148‑1 (MQ=255)
                                                                aCAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGa                                                                                   <  3:199509/149‑1 (MQ=255)
                                                                    ccACAAAATGGACATGGTTTAACATTGTCATATGTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTTCACTGTATTCGACGGTTAATACAGAGGAATCt                                                                               >  2:76928/1‑149 (MQ=255)
                                                                     cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTg                                                                              >  8:191845/1‑149 (MQ=255)
                                                                        aaaaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAg                                                                           >  5:184205/1‑149 (MQ=255)
                                                                              gACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                 <  5:343004/105‑1 (MQ=255)
                                                                              gACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                                                                                 >  6:343004/1‑105 (MQ=255)
                                                                              gACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGa                                                                     <  6:210319/149‑1 (MQ=255)
                                                                               aCATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAt                                                                    <  7:313148/149‑1 (MQ=255)
                                                                                cATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAtt                                                                   <  5:340620/149‑1 (MQ=255)
                                                                                   ggTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACCCCGCCAGGCTGAATTATTCCTCTGAATTATCGATTTCACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGt                                                                 >  2:190240/1‑148 (MQ=255)
                                                                                    gTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCa                                                               <  2:13852/149‑1 (MQ=255)
                                                                                     tttAACATTGTCATCTCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAg                                                              <  3:227311/149‑1 (MQ=255)
                                                                                      ttAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCACTGAATTATCGATTACACTGTATTCCCCGCTTAATACAGAGGAATCTGCAGGATCGATTGTCAGt                                                             >  2:64349/1‑149 (MQ=255)
                                                                                        taCATTGTCATATCTCTTAATTTTTCTCATAAAAAATATTTCATGTTGGCGGTGCTTTACACTGCCAGTCTGAATTTTTCCTCTGATTTATCTATTACAGTGTATTCCCCGGGTAATACAGAGGAATCTGGAGGATGGATTGTCAGTg                                                            <  8:344800/147‑1 (MQ=255)
                                                                                         aCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGt                                                          <  4:150191/149‑1 (MQ=255)
                                                                                          cATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGtt                                                         <  6:184205/149‑1 (MQ=255)
                                                                                           tttGTCATATCTAATAATTTTTATCATAAAAAATATTTCAAGTTGGCGGTTAATTACACCGCCAGGCTGAATTATTCCTCTGATTTATCGATTACACTGTATTCCCCGGTTAATACa                                                                                        <  2:340938/116‑1 (MQ=255)
                                                                                           aTTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGACTACACTGTATTCCCCGGTTAATACAGAGGa                                                                                   <  4:295939/122‑1 (MQ=255)
                                                                                           aTTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGACTACACTGTATTCCCCGGTTAATACAGAGGa                                                                                   >  3:295939/1‑122 (MQ=255)
                                                                                           aTTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTACTCTGAATTATCGATTACACTGTATTCCCCGGTTAATaaa                                                                                        >  1:340938/1‑115 (MQ=255)
                                                                                            ttGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc                                                       >  4:288909/1‑149 (MQ=255)
                                                                                                  tatCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCa                                                 <  6:187793/149‑1 (MQ=255)
                                                                                                  tatCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGACAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCa                                                 >  7:323035/1‑149 (MQ=255)
                                                                                                      tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTccc                                                                                                   <  3:125333/95‑1 (MQ=255)
                                                                                                      tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTccc                                                                                                   >  4:125333/1‑95 (MQ=255)
                                                                                                      tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCa                                             >  2:406250/1‑149 (MQ=255)
                                                                                                        aTAATTTTTCTCTTAAAATATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTGCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCAtt                                           <  5:391095/149‑1 (MQ=255)
                                                                                                         tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTTAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTg                                          <  5:160004/149‑1 (MQ=255)
                                                                                                         tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt                                                                                                           <  7:101690/84‑1 (MQ=255)
                                                                                                         tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt                                                                                                           >  8:101690/1‑84 (MQ=255)
                                                                                                         aaaTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGATTTATTCCTATGATTTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTTCATGATCGATTGTCAGTGGATCAATTTCATCCAtt                                           >  7:372992/2‑148 (MQ=255)
                                                                                                           aTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGa                                         <  1:126220/148‑1 (MQ=255)
                                                                                                            tttttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGAt                                        >  1:152548/1‑148 (MQ=255)
                                                                                                               ttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACt                                     <  8:50017/148‑1 (MQ=255)
                                                                                                                    aTAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGc                               >  6:352198/1‑149 (MQ=255)
                                                                                                                     tAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAt                                                                    <  1:41344/111‑1 (MQ=255)
                                                                                                                     tAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCt                              >  2:68341/1‑149 (MQ=255)
                                                                                                                       aaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTgg                            <  2:152548/149‑1 (MQ=255)
                                                                                                                         aaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTTATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGAt                          >  2:216553/1‑149 (MQ=255)
                                                                                                                          aaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTCTTCCTCTGAATTATCGATTACACTGTATTCCCCGGtt                                                                                              <  6:274534/80‑1 (MQ=255)
                                                                                                                          aaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACATAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGAtc                         <  1:87099/149‑1 (MQ=255)
                                                                                                                          aaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGAtc                         >  3:323006/1‑149 (MQ=255)
                                                                                                                              tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCaa                     >  1:18757/1‑149 (MQ=255)
                                                                                                                                 cAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTTAATTATCGATTACACTGTATTCCCCGGTTAATAGAGAGTAATCTGCAGTATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAAtt                   <  1:64349/148‑1 (MQ=255)
                                                                                                                                    gTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa               <  2:243577/149‑1 (MQ=255)
                                                                                                                                    gTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa               <  4:187274/149‑1 (MQ=255)
                                                                                                                                    gTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa               <  5:284869/149‑1 (MQ=255)
                                                                                                                                                ttACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa               >  2:77765/1‑137 (MQ=255)
                                                                                                                                                ttACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATa               <  1:77765/137‑1 (MQ=255)
                                                                                                                                                ttACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATAtt   >  1:97617/1‑149 (MQ=255)
                                                                                                                                                  acacCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATAttt  <  5:390198/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTT  >  NC_000913/1413822‑1414115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: