Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,695,367	T→C	5.1%	Q235Q (CAA→CAG)	uidA ←	beta‑D‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,367	0	T	C	5.1%	243.5 / 3.3	78	Q235Q (CAA→CAG)	uidA	beta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (39/34);  new base C (2/2);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

GGTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATAT  >  NC_000913/1695219‑1695515                                                                                                                                                     |                                                                                                                                                     ggTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACt                                                                                                                                                      >  8:231290/1‑149 (MQ=255)  gTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACtt                                                                                                                                                     <  2:271967/149‑1 (MQ=255)  gTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACtt                                                                                                                                                     <  2:270403/149‑1 (MQ=255)       tCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATCACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCa                                                                                                                                                  >  8:96468/1‑147 (MQ=255)       tCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCaaa                                                                                                                                                <  8:313598/149‑1 (MQ=255)       tCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCa                                                                                                                                                  >  5:163879/1‑147 (MQ=255)       tCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCa                                                                                                                                                  >  7:170006/1‑147 (MQ=255)         aGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGt                                                                                                                                              >  2:129146/1‑149 (MQ=255)            aaCTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTccc                                                                                                                                           >  2:7494/1‑149 (MQ=255)                   cGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGt                                                                                                                                     <  7:374438/148‑1 (MQ=255)                      ccTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCt                                                                                                                                 >  2:352331/1‑149 (MQ=255)                       cTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCtt                                                                                                                                <  7:231290/149‑1 (MQ=255)                           actgccactgccCGGATGCCGACGCGAAGCGGGTAGATATCACCCTCTGTCTGGCTTTTCGCGGTGACGCACAGATCATAGAGATAACCTTCACCCGGCTGCCAGAGGGGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTc                                                                                                                             <  4:320771/148‑1 (MQ=255)                           aCTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTc                                                                                                                             <  8:2888/148‑1 (MQ=255)                           aCTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTc                                                                                                                             <  6:131334/148‑1 (MQ=255)                            cTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCa                                                                                                                           <  1:7494/149‑1 (MQ=255)                             tGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCaa                                                                                                                                                 >  6:202575/1‑126 (MQ=255)                               ccACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        <  1:406561/149‑1 (MQ=255)                               ccACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        <  8:299727/149‑1 (MQ=255)                               ccACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        <  1:129146/149‑1 (MQ=255)                                     aCCGGATGCCGACGCGAAGAGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACTGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCGCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   <  2:101857/148‑1 (MQ=255)                                       cGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCTGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAaccac                                                                                                                <  4:99965/149‑1 (MQ=255)                                        ggATGCCGACGCCAACCGGGTAGATATCACACTCTGTCTGGCTATTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAaccac                                                                                                                >  4:64410/1‑148 (MQ=255)                                         gATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAATTCCCGCTATTGCCTTGTCCAGTTGCAACCCCCt                                                                                                              >  8:160162/1‑149 (MQ=255)                                         gATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCt                                                                                                              <  5:89148/149‑1 (MQ=255)                                            gCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGt                                                                                                            <  1:23138/148‑1 (MQ=255)                                             ccGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGtt                                                                                                           <  8:351377/148‑1 (MQ=255)                                               gACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGAt                                                                                                        >  4:243866/1‑149 (MQ=255)                                                          ggTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACg                                                                                              >  5:126662/1‑148 (MQ=255)                                                                    acacTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAg                                                                                                                          >  2:277141/1‑110 (MQ=255)                                                                      acTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        <  7:145241/110‑1 (MQ=255)                                                                      acTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        >  8:145241/1‑110 (MQ=255)                                                                           gtctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACAt                                                                             >  7:142284/1‑148 (MQ=255)                                                                            tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACAt                                                                             <  5:161517/147‑1 (MQ=255)                                                                            tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACAt                                                                             >  6:161517/1‑147 (MQ=255)                                                                              tgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACCTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   >  5:276566/1‑107 (MQ=255)                                                                              tgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACCTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   >  5:276568/1‑107 (MQ=255)                                                                              tgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACCTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   <  6:276566/107‑1 (MQ=255)                                                                              gggCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACCTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   <  6:276568/106‑1 (MQ=255)                                                                                gCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCAt                                                                       >  4:274553/1‑149 (MQ=255)                                                                                    ttGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTg                                                                     <  6:163879/147‑1 (MQ=255)                                                                                         tgtgACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACACCCCCATTGGccacca                                                              >  6:155913/1‑149 (MQ=255)                                                                                              cGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGc                                                         <  1:352331/149‑1 (MQ=255)                                                                                               gCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCa                                                                           <  5:10401/130‑1 (MQ=255)                                                                                               gCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCa                                                                           >  6:10401/1‑130 (MQ=255)                                                                                                cacaGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCa                                                       <  8:170006/149‑1 (MQ=255)                                                                                                   aGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTc                                                    <  3:64410/149‑1 (MQ=255)                                                                                                   aGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTc                                                    <  6:126662/149‑1 (MQ=255)                                                                                                     ttCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCaa                                                  >  2:298013/1‑149 (MQ=255)                                                                                                     ttCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGACCAGTTGCAACCACCTGGTGATCCGCATCACGCAGTTCAACGCGGACATCACCATTGGCCACCACCTGCCAGTCaa                                                  <  2:107294/149‑1 (MQ=255)                                                                                                        aTAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCaa                                                  >  7:233848/1‑146 (MQ=255)                                                                                                            agaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGAc                                                                               <  4:208009/113‑1 (MQ=255)                                                                                                            agaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGAc                                                                               >  3:208009/1‑113 (MQ=255)                                                                                                            agaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGAcgc                                           >  6:88124/1‑149 (MQ=255)                                                                                                            agaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGAcgc                                           <  3:274553/149‑1 (MQ=255)                                                                                                              aTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGCTCCGCATCACGCAGTTCACCGCTGCCATCCCCATTGGCCACCACCTGCCAGTCCACAGACGCGt                                         >  1:383919/1‑149 (MQ=255)                                                                                                              aTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGt                                         >  6:12584/1‑149 (MQ=255)                                                                                                              aTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGAACCGCATCACGCAGTTCAACGCAGACATCACCATTCGCCAACACCAGCCAGTCAACAGACGCGt                                         >  8:269996/1‑149 (MQ=255)                                                                                                               tAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTg                                        <  7:160162/149‑1 (MQ=255)                                                                                                                 aCCTTCACCCGGTTGCCAGTGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTgg                                       <  1:379283/148‑1 (MQ=255)                                                                                                                 aCCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCAtt                                                                      <  8:21204/117‑1 (MQ=255)                                                                                                                 aCCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCAtt                                                                      >  7:21204/1‑117 (MQ=255)                                                                                                                   cTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTa                                    >  3:136103/1‑149 (MQ=255)                                                                                                                      cACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACa                                  <  1:298013/148‑1 (MQ=255)                                                                                                                       aCCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGt                                >  6:44953/1‑149 (MQ=255)                                                                                                                       aCCCGGTTGCCAGAGGTGCGGATTCACCACGTGCAAAGTCCCGCTAGTGCCTTGTCCAGCTGCAACCCCCTGTTGCTCCGCATCCCGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGGTACAGt                                >  8:357601/1‑149 (MQ=255)                                                                                                                          cGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCtt                             >  7:127094/1‑149 (MQ=255)                                                                                                                                ccAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGa                       >  1:250980/1‑149 (MQ=255)                                                                                                                                          ggATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCCCCCCCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTcac             >  3:294535/1‑149 (MQ=255)                                                                                                                                               caccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACgg        >  6:245935/1‑149 (MQ=255)                                                                                                                                                accacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGCCATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGt       >  2:294241/1‑149 (MQ=255)                                                                                                                                                 ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTg                                                                     >  1:361869/1‑86 (MQ=255)                                                                                                                                                 ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTg                                                                     <  2:361869/86‑1 (MQ=255)                                                                                                                                                 ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTc               <  5:397062/140‑1 (MQ=255)                                                                                                                                                 ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTc               >  6:397062/1‑140 (MQ=255)                                                                                                                                                 ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGt       >  6:145841/1‑148 (MQ=255)                                                                                                                                                  cacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTTCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACAACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGAGCGACATGCGTCACCACGGTGa     >  7:158198/1‑149 (MQ=255)                                                                                                                                                  cacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGAGATGCGTCACCACGGTGa     <  5:88124/149‑1 (MQ=255)                                                                                                                                                  cacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGa     >  3:389050/1‑149 (MQ=255)                                                                                                                                                   acTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGat    <  7:96468/149‑1 (MQ=255)                                                                                                                                                    cTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGata   >  7:376923/1‑149 (MQ=255)                                                                                                                                                     ttGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGatat  >  6:300185/1‑149 (MQ=255)                                                                                                                                                     ttGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGatat  >  2:65625/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GGTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATAT  >  NC_000913/1695219‑1695515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑