Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,695,367 T→C 5.1% Q235Q (CAA→CAG uidA ← beta‑D‑glucuronidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,695,3670TC 5.1% 243.5 / 3.3 78Q235Q (CAA→CAGuidAbeta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (39/34);  new base C (2/2);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

GGTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATAT  >  NC_000913/1695219‑1695515
                                                                                                                                                    |                                                                                                                                                    
ggTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACt                                                                                                                                                      >  8:231290/1‑149 (MQ=255)
 gTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACtt                                                                                                                                                     <  2:271967/149‑1 (MQ=255)
 gTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACtt                                                                                                                                                     <  2:270403/149‑1 (MQ=255)
      tCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATCACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCa                                                                                                                                                  >  8:96468/1‑147 (MQ=255)
      tCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCaaa                                                                                                                                                <  8:313598/149‑1 (MQ=255)
      tCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCa                                                                                                                                                  >  5:163879/1‑147 (MQ=255)
      tCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCa                                                                                                                                                  >  7:170006/1‑147 (MQ=255)
        aGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGt                                                                                                                                              >  2:129146/1‑149 (MQ=255)
           aaCTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTccc                                                                                                                                           >  2:7494/1‑149 (MQ=255)
                  cGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGt                                                                                                                                     <  7:374438/148‑1 (MQ=255)
                     ccTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCt                                                                                                                                 >  2:352331/1‑149 (MQ=255)
                      cTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCtt                                                                                                                                <  7:231290/149‑1 (MQ=255)
                          actgccactgccCGGATGCCGACGCGAAGCGGGTAGATATCACCCTCTGTCTGGCTTTTCGCGGTGACGCACAGATCATAGAGATAACCTTCACCCGGCTGCCAGAGGGGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTc                                                                                                                             <  4:320771/148‑1 (MQ=255)
                          aCTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTc                                                                                                                             <  8:2888/148‑1 (MQ=255)
                          aCTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTc                                                                                                                             <  6:131334/148‑1 (MQ=255)
                           cTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCa                                                                                                                           <  1:7494/149‑1 (MQ=255)
                            tGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCaa                                                                                                                                                 >  6:202575/1‑126 (MQ=255)
                              ccACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        <  1:406561/149‑1 (MQ=255)
                              ccACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        <  8:299727/149‑1 (MQ=255)
                              ccACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        <  1:129146/149‑1 (MQ=255)
                                    aCCGGATGCCGACGCGAAGAGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACTGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCGCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   <  2:101857/148‑1 (MQ=255)
                                      cGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCTGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAaccac                                                                                                                <  4:99965/149‑1 (MQ=255)
                                       ggATGCCGACGCCAACCGGGTAGATATCACACTCTGTCTGGCTATTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAaccac                                                                                                                >  4:64410/1‑148 (MQ=255)
                                        gATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAATTCCCGCTATTGCCTTGTCCAGTTGCAACCCCCt                                                                                                              >  8:160162/1‑149 (MQ=255)
                                        gATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCt                                                                                                              <  5:89148/149‑1 (MQ=255)
                                           gCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGt                                                                                                            <  1:23138/148‑1 (MQ=255)
                                            ccGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGtt                                                                                                           <  8:351377/148‑1 (MQ=255)
                                              gACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGAt                                                                                                        >  4:243866/1‑149 (MQ=255)
                                                         ggTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACg                                                                                              >  5:126662/1‑148 (MQ=255)
                                                                   acacTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAg                                                                                                                          >  2:277141/1‑110 (MQ=255)
                                                                     acTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        <  7:145241/110‑1 (MQ=255)
                                                                     acTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGtt                                                                                                                        >  8:145241/1‑110 (MQ=255)
                                                                          gtctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACAt                                                                             >  7:142284/1‑148 (MQ=255)
                                                                           tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACAt                                                                             <  5:161517/147‑1 (MQ=255)
                                                                           tctgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACAt                                                                             >  6:161517/1‑147 (MQ=255)
                                                                             tgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACCTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   >  5:276566/1‑107 (MQ=255)
                                                                             tgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACCTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   >  5:276568/1‑107 (MQ=255)
                                                                             tgGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACCTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   <  6:276566/107‑1 (MQ=255)
                                                                             gggCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACCTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAac                                                                                                                   <  6:276568/106‑1 (MQ=255)
                                                                               gCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCAt                                                                       >  4:274553/1‑149 (MQ=255)
                                                                                   ttGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTg                                                                     <  6:163879/147‑1 (MQ=255)
                                                                                        tgtgACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACACCCCCATTGGccacca                                                              >  6:155913/1‑149 (MQ=255)
                                                                                             cGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGc                                                         <  1:352331/149‑1 (MQ=255)
                                                                                              gCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCa                                                                           <  5:10401/130‑1 (MQ=255)
                                                                                              gCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCa                                                                           >  6:10401/1‑130 (MQ=255)
                                                                                               cacaGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCa                                                       <  8:170006/149‑1 (MQ=255)
                                                                                                  aGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTc                                                    <  3:64410/149‑1 (MQ=255)
                                                                                                  aGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTc                                                    <  6:126662/149‑1 (MQ=255)
                                                                                                    ttCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCaa                                                  >  2:298013/1‑149 (MQ=255)
                                                                                                    ttCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGACCAGTTGCAACCACCTGGTGATCCGCATCACGCAGTTCAACGCGGACATCACCATTGGCCACCACCTGCCAGTCaa                                                  <  2:107294/149‑1 (MQ=255)
                                                                                                       aTAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCaa                                                  >  7:233848/1‑146 (MQ=255)
                                                                                                           agaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGAc                                                                               <  4:208009/113‑1 (MQ=255)
                                                                                                           agaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGAc                                                                               >  3:208009/1‑113 (MQ=255)
                                                                                                           agaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGAcgc                                           >  6:88124/1‑149 (MQ=255)
                                                                                                           agaTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGAcgc                                           <  3:274553/149‑1 (MQ=255)
                                                                                                             aTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGCTCCGCATCACGCAGTTCACCGCTGCCATCCCCATTGGCCACCACCTGCCAGTCCACAGACGCGt                                         >  1:383919/1‑149 (MQ=255)
                                                                                                             aTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGt                                         >  6:12584/1‑149 (MQ=255)
                                                                                                             aTAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGAACCGCATCACGCAGTTCAACGCAGACATCACCATTCGCCAACACCAGCCAGTCAACAGACGCGt                                         >  8:269996/1‑149 (MQ=255)
                                                                                                              tAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTg                                        <  7:160162/149‑1 (MQ=255)
                                                                                                                aCCTTCACCCGGTTGCCAGTGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTgg                                       <  1:379283/148‑1 (MQ=255)
                                                                                                                aCCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCAtt                                                                      <  8:21204/117‑1 (MQ=255)
                                                                                                                aCCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCAtt                                                                      >  7:21204/1‑117 (MQ=255)
                                                                                                                  cTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTa                                    >  3:136103/1‑149 (MQ=255)
                                                                                                                     cACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACa                                  <  1:298013/148‑1 (MQ=255)
                                                                                                                      aCCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGt                                >  6:44953/1‑149 (MQ=255)
                                                                                                                      aCCCGGTTGCCAGAGGTGCGGATTCACCACGTGCAAAGTCCCGCTAGTGCCTTGTCCAGCTGCAACCCCCTGTTGCTCCGCATCCCGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGGTACAGt                                >  8:357601/1‑149 (MQ=255)
                                                                                                                         cGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCtt                             >  7:127094/1‑149 (MQ=255)
                                                                                                                               ccAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGa                       >  1:250980/1‑149 (MQ=255)
                                                                                                                                         ggATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCCCCCCCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTcac             >  3:294535/1‑149 (MQ=255)
                                                                                                                                              caccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACgg        >  6:245935/1‑149 (MQ=255)
                                                                                                                                               accacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGCCATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGt       >  2:294241/1‑149 (MQ=255)
                                                                                                                                                ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTg                                                                     >  1:361869/1‑86 (MQ=255)
                                                                                                                                                ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTg                                                                     <  2:361869/86‑1 (MQ=255)
                                                                                                                                                ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTc               <  5:397062/140‑1 (MQ=255)
                                                                                                                                                ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTc               >  6:397062/1‑140 (MQ=255)
                                                                                                                                                ccacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGt       >  6:145841/1‑148 (MQ=255)
                                                                                                                                                 cacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTTCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACAACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGAGCGACATGCGTCACCACGGTGa     >  7:158198/1‑149 (MQ=255)
                                                                                                                                                 cacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGAGATGCGTCACCACGGTGa     <  5:88124/149‑1 (MQ=255)
                                                                                                                                                 cacTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGa     >  3:389050/1‑149 (MQ=255)
                                                                                                                                                  acTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGat    <  7:96468/149‑1 (MQ=255)
                                                                                                                                                   cTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGata   >  7:376923/1‑149 (MQ=255)
                                                                                                                                                    ttGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGatat  >  6:300185/1‑149 (MQ=255)
                                                                                                                                                    ttGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGatat  >  2:65625/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTTAATCAGGAACTGTTCGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCACACTCTGTCTGGCTTTTGGCTGTGACGCACAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATAT  >  NC_000913/1695219‑1695515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: