Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,024,612 C→A 5.5% intergenic (+21/+23) rcsA → / ← dsrB transcriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,024,6120CA 5.5% 341.3 / 5.8 99intergenic (+21/+23)rcsA/dsrBtranscriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (39/54);  new base A (3/3);  total (42/57)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

Only 100 of 103 total aligned reads displayed.

CCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAG  >  NC_000913/2024464‑2024760
                                                                                                                                                    |                                                                                                                                                    
ccAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTc                                                                                                                                                      <  6:243626/149‑1 (MQ=255)
  aaGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTccc                                                                                                                                                    <  4:372755/149‑1 (MQ=255)
  aaGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTcc                                                                                                                                                     >  2:262557/1‑148 (MQ=255)
    gACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCccac                                                                                                                                                  >  4:146836/1‑149 (MQ=255)
    gACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCccac                                                                                                                                                  <  6:287639/149‑1 (MQ=255)
      ccGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCccacca                                                                                                                                                <  7:373731/149‑1 (MQ=255)
        gTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGt                                                                                                                                              >  4:181812/1‑149 (MQ=255)
         tttCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTc                                                                                                                                             <  8:301482/149‑1 (MQ=255)
           tCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAg                                                                                                                                           >  5:197223/1‑149 (MQ=255)
           tCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGGAAACCACCAGTCa                                                                                                                                            <  6:109473/148‑1 (MQ=255)
           tCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGGAAACCACCAGTCa                                                                                                                                            <  4:242987/148‑1 (MQ=255)
             aTCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGc                                                                                                                                         >  6:240999/1‑149 (MQ=255)
              tCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCt                                                                                                                                        >  7:253586/1‑149 (MQ=255)
              tCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCt                                                                                                                                        >  5:399007/1‑149 (MQ=255)
              tCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCt                                                                                                                                        <  5:116983/149‑1 (MQ=255)
                     aaGGTAATATTAAACGTAAGAGCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTATGGTATTTTTGTCAACATGCTCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAg                                                                                                                                 <  6:198450/148‑1 (MQ=255)
                      aGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGa                                                                                                                                <  8:366381/149‑1 (MQ=255)
                      aGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGa                                                                                                                                <  5:315668/149‑1 (MQ=255)
                      aGGTAATATTAAACCTAAGATCAAAACGCATAATAAACAGGCTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGa                                                                                                                                <  6:54552/149‑1 (MQ=255)
                        gTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGGGGTTTCCCACCAGTCAGGCTGAATAAGAtt                                                                                                                              <  3:29787/149‑1 (MQ=255)
                         tAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTa                                                                                                                             <  4:363154/149‑1 (MQ=255)
                         tAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTa                                                                                                                             <  1:262557/149‑1 (MQ=255)
                            tatTAAACGTAAGATCAAAAAGCATAATAAACAGGTTATCTACAATGTCTTCCGACTGACGGATAATGTGACTACTGGTATTTTTGTCAACATGCGCTAACACCATCTGACTGGTGCTTTACCACCAGTCAGGCTGAATAAGATTActa                                                                                                                          >  6:274948/1‑148 (MQ=255)
                             atTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTActct                                                                                                                         >  4:165945/1‑149 (MQ=255)
                               tAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGc                                                                                                                       >  5:139299/1‑149 (MQ=255)
                                  aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGGCGTCCGACTGACGGATAAGGTGACTAATTGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCttt                                                                                                                    <  5:330232/149‑1 (MQ=255)
                                    gTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtct                                                                                                                  >  6:235361/1‑149 (MQ=255)
                                      aaGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtctc                                                                                                                 >  1:218544/1‑148 (MQ=255)
                                         aTCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCcac                                                                                                              >  2:356038/1‑148 (MQ=255)
                                          tCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACaa                                                                                                            <  6:68089/149‑1 (MQ=255)
                                             aaaCGCTTAATAAACAGGTTATCTTCCTATTCGTCCGACTGTGTGAAAATGTGATTAATGGTATTTTTGTCTAGATGCGGTAACTCATTCTGACTGGTGGTTTCCCAGCAGTGAGGGTGATTAAGATTACTCTGCTTTCTCCACTAAGa                                                                                                         <  2:187937/149‑1 (MQ=255)
                                               aCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATa                                                                                                       <  1:215232/149‑1 (MQ=255)
                                                 gCATAATAAACAGGTTATCTACCATGTCCTCCGACTGACGGATAATGTGACTAATCGTATATTTGTAAACATGCGCAAACACATTCTGAATGGTGGTTTCCCACCAGTCAGGCTGAATAAGATAACTCTCCATTCTCCACAAACATAcc                                                                                                     >  5:43750/1‑149 (MQ=255)
                                                   ataataAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGt                                                                                                   >  2:94824/1‑149 (MQ=255)
                                                     aataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTcc                                                                                                 >  3:123110/1‑149 (MQ=255)
                                                     aataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTcc                                                                                                 >  6:190708/1‑149 (MQ=255)
                                                      ataaACAGGTTATCTACCATGTCTGCCGACTGACGGATAATGTGACTACTGGTATTTTTGTCAACATGCGCTAACCCATTCTGACTGGTGGGTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTccg                                                                                                <  5:274948/149‑2 (MQ=255)
                                                      ataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCt                                                                                                <  7:395017/149‑1 (MQ=255)
                                                         aaCAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGAt                                                                                             <  3:307065/149‑1 (MQ=255)
                                                               ttATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTg                                                                                       >  4:93614/1‑149 (MQ=255)
                                                                     aCCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAt                                                                                 >  4:245834/1‑149 (MQ=255)
                                                                     aCCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAt                                                                                 >  5:214732/1‑149 (MQ=255)
                                                                      ccATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTc                                                                                   <  2:218544/146‑1 (MQ=255)
                                                                        aTGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTaa                                                                              <  6:139299/149‑1 (MQ=255)
                                                                         tGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTaaa                                                                             <  3:245834/149‑1 (MQ=255)
                                                                           tcgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAg                                                                            <  7:193298/148‑1 (MQ=255)
                                                                              tcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAcc                                                                        <  1:356038/149‑1 (MQ=255)
                                                                                cGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCa                                                                       <  3:387695/148‑1 (MQ=255)
                                                                                 gACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAc                                                                         >  7:205937/1‑145 (MQ=255)
                                                                                 gACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGa                                                                     <  6:399007/149‑1 (MQ=255)
                                                                                  aCTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGAt                                                                    <  5:240999/149‑1 (MQ=255)
                                                                                    tGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGc                                                                  >  8:6599/1‑149 (MQ=255)
                                                                                    tGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGc                                                                  >  8:106750/1‑149 (MQ=255)
                                                                                        ggATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAg                                                              >  7:114671/1‑149 (MQ=255)
                                                                                           tAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCgg                                                           <  3:165945/149‑1 (MQ=255)
                                                                                             aTGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCggg                                                          <  1:401761/148‑1 (MQ=255)
                                                                                             aTGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCggg                                                          <  3:181812/148‑1 (MQ=255)
                                                                                             aTGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGt                                                         <  6:181284/149‑1 (MQ=255)
                                                                                              tgtgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTTATTTAGTTTACTCTGCTTTCTCCACATTGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCGGAGgggggt                                                        <  6:375085/149‑3 (MQ=255)
                                                                                                  aCTAATGGTATTTTTGTCAACATGCGCTATCACATTCTTACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGCACCAGATGCCGAGCGGGTAGTCt                                                    >  5:379613/1‑149 (MQ=255)
                                                                                                  aCTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTc                                                     <  5:235361/148‑1 (MQ=255)
                                                                                                    tAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACaaa                                                                                                           <  5:339080/92‑1 (MQ=255)
                                                                                                    tAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACaaa                                                                                                           >  6:339080/1‑92 (MQ=255)
                                                                                                    tAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGCACCAGATGCCGAGCGGGTAGTCtgc                                                  >  4:34318/1‑147 (MQ=255)
                                                                                                      aTGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTc                                                  <  7:202684/147‑1 (MQ=255)
                                                                                                      aTGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTc                                                  <  2:240979/147‑1 (MQ=255)
                                                                                                      aTGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTc                                                  >  5:400637/1‑147 (MQ=255)
                                                                                                      aTGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTc                                                  <  8:156262/147‑1 (MQ=255)
                                                                                                       tGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTAct                                                                                                                           >  5:316274/1‑73 (MQ=255)
                                                                                                       tGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTAct                                                                                                                           <  6:316274/73‑1 (MQ=255)
                                                                                                       tGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTa                                                        >  2:236764/1‑140 (MQ=255)
                                                                                                       tGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTa                                                        <  1:236764/140‑1 (MQ=255)
                                                                                                        ggTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGc                                              >  3:39399/1‑149 (MQ=255)
                                                                                                         gTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGc                                              <  3:146836/148‑1 (MQ=255)
                                                                                                         gTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGc                                              <  1:94824/148‑1 (MQ=255)
                                                                                                           aTTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGa                                            >  7:322072/1‑148 (MQ=255)
                                                                                                              tttGTCTACATGCGCTAACACATTCTGTCTGGTGGTTTACCTCCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTTATGCCCTGCTTCATTAAATAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAAc                                         <  3:255847/148‑1 (MQ=255)
                                                                                                              tttGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAAc                                         <  3:93614/148‑1 (MQ=255)
                                                                                                                tGTCAACATGCTCTTACACATTCTGACTGGTGGTTTCGCACCAGTCAGGAGGAATAAGTTTACTCTGCTTTCTCCACAAAGATTCCGTCCTTATGCCCTGCTTCATTAAATAACCAGATGCAGAGCGGGTAGTCTTCCAGCTAAACCa                                       <  6:43750/148‑1 (MQ=255)
                                                                                                                tGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCa                                       <  2:184790/148‑1 (MQ=255)
                                                                                                                  tCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAACGAAACCAGGt                                    <  2:311290/149‑1 (MQ=255)
                                                                                                                     aCATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCGTTCTCCACCAAGATACCGTCCAGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACa                                 <  1:292605/149‑1 (MQ=255)
                                                                                                                       aTGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACAtt                               <  1:96137/149‑1 (MQ=255)
                                                                                                                          cgcTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATtgt                             <  8:170166/148‑1 (MQ=255)
                                                                                                                              aaCACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCtt                        >  7:282509/1‑149 (MQ=255)
                                                                                                                               acacATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCtt                        <  7:6599/148‑1 (MQ=255)
                                                                                                                               acacATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCtt                        <  8:114671/148‑1 (MQ=255)
                                                                                                                                cacaTTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCa                      <  8:205937/149‑1 (MQ=255)
                                                                                                                                  caTTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCAc                     >  6:106977/1‑148 (MQ=255)
                                                                                                                                   aTTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACt                    >  1:8208/1‑148 (MQ=255)
                                                                                                                                    ttCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTa                   >  2:252543/1‑148 (MQ=255)
                                                                                                                                      cTGACTGGTGGGAAACCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAACCCAGGTACATTGTGCCTTCACTaacc                >  8:298994/1‑147 (MQ=255)
                                                                                                                                       tGACTGGTGGGAAACCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAct               >  3:317052/1‑149 (MQ=255)
                                                                                                                                         aCTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcc             >  6:400842/1‑149 (MQ=255)
                                                                                                                                           tggtggTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGGCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGGCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcct            >  2:218765/1‑148 (MQ=255)
                                                                                                                                             gtggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCTGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcctc           <  1:80748/147‑1 (MQ=255)
                                                                                                                                             gtggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCaa         <  5:106977/149‑1 (MQ=255)
                                                                                                                                                gtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTg      >  4:183704/1‑149 (MQ=255)
                                                                                                                                                gtTTCCCACCAGTAAGTCTGAATAAGATTAATCTGCTTTATAAAAAAAGATACAGTCCTGATGCCCTACTTCATTAAAGAACAAGATTAAGAGCGGGTATTCTTCCAGAGAAACCATGTACATTTTTCCTTCACTAAACTCCTCAACt       >  4:57852/1‑148 (MQ=255)
                                                                                                                                                  ttCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGc     >  5:236681/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAG  >  NC_000913/2024464‑2024760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: