Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,123,637 T→A 5.4% E295V (GAG→GTG)  cpsB ← mannose‑1‑phosphate guanyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,123,6370TA 5.4% 282.5 / 5.9 93E295V (GAG→GTG) cpsBmannose‑1‑phosphate guanyltransferase
Reads supporting (aligned to +/- strand):  ref base T (45/43);  new base A (2/3);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01

ACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGCTTCTT  >  NC_000913/2123495‑2123784
                                                                                                                                              |                                                                                                                                                   
aCGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTcggcg                                                                                                                                                <  1:243719/148‑1 (MQ=255)
aCGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTcggcg                                                                                                                                                <  1:69447/148‑1 (MQ=255)
 cGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCTCCCTGGCAAAAGTTGACCTCGGCGgt                                                                                                                                              >  6:160011/1‑149 (MQ=255)
 cGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACACCGCCGAGGGCAACGTTTGCCAcggcgat                                                                                                                                              <  7:137518/149‑3 (MQ=255)
   gTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGGTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGgtgt                                                                                                                                            >  4:180542/1‑149 (MQ=255)
    tCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGtgtg                                                                                                                                           <  5:135888/149‑1 (MQ=255)
         aaTCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGgcgc                                                                                                                                      <  5:58337/149‑1 (MQ=255)
          aTCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCt                                                                                                                                     >  4:278708/1‑149 (MQ=255)
          aTCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACACCGCCGAGGGCAACGTTTGCCACGGCGAtgtgattaat                                                                                                                                     <  1:338001/149‑7 (MQ=37)
           tCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTcg                                                                                                                                                   >  4:262793/1‑134 (MQ=255)
           tCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTcg                                                                                                                                                   <  3:262793/134‑1 (MQ=255)
             aGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGa                                                                                                                                   <  2:361865/148‑1 (MQ=255)
              gCAGCGCATCTTTGGTCTGCACCACTACCAGATCTTTCCCCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGCCAAACGTGGCCCTCGGCGGGGTGGGCGCTGAtc                                                                                                                                 <  2:205930/149‑1 (MQ=255)
                aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGAtctc                                                                                                                               >  4:213894/1‑149 (MQ=255)
                 ccGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTcc                                                                                                                              >  5:623/1‑149 (MQ=255)
                    cATCTTTGGTCTGCACCACTACCAGATCTTTCACCACGACGGTGGAGACCAGGACAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCa                                                                                                                            >  4:263605/1‑148 (MQ=255)
                        tttGGTCTGCACCACTACCAGATCTTTCCCCCCCACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTATTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCACTCGGCGGTGTggg                                                                                                                                         >  1:25757/1‑131 (MQ=255)
                          tGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATaa                                                                                                                         <  8:179076/145‑1 (MQ=255)
                           ggTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGaa                                                                                                                     <  1:192723/148‑1 (MQ=255)
                            gTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGa                                                                                                                   <  2:354005/149‑1 (MQ=255)
                             tCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGAc                                                                                                                  <  3:213894/149‑1 (MQ=255)
                                gCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAg                                                                                                               >  3:315760/1‑149 (MQ=255)
                                gCACCACTACCAGATATTTCACCCCGACGGTCGTGACCACGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATCAACACCAg                                                                                                               >  4:200160/1‑149 (MQ=255)
                                 caccacTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCa                                                                                                                <  6:623/147‑1 (MQ=255)
                                 caccacTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAg                                                                                                               <  1:105081/148‑1 (MQ=255)
                                  accacTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAg                                                                                                               >  2:331902/1‑147 (MQ=255)
                                  accacTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGa                                                                                                             >  5:292117/1‑149 (MQ=255)
                                  accacTACCAGATCTTTAACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCCGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAg                                                                                                               >  5:297835/1‑147 (MQ=255)
                                   ccacTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAg                                                                                                            >  7:279746/1‑149 (MQ=255)
                                    cacTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGc                                                                                                           >  8:17977/1‑149 (MQ=255)
                                    cacTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGc                                                                                                           <  1:125149/149‑1 (MQ=255)
                                    cacTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGc                                                                                                           <  7:184320/149‑1 (MQ=255)
                                     acTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGc                                                                                                           >  8:155765/1‑148 (MQ=255)
                                      cTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCa                                                                                                         <  4:196895/149‑1 (MQ=255)
                                         ccAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCaa                                                                                                        <  1:275054/147‑1 (MQ=255)
                                          cAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACAt                                                                                                     >  5:173205/1‑149 (MQ=255)
                                          cAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACACCGCCGAGGGCAACGTTTGCCACGGCGatgt                                                                                                                                            <  7:312348/110‑1 (MQ=37)
                                          cAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACACCGCCGAGGGCAACGTTTGCCACGGCGatgt                                                                                                                                            >  8:312348/1‑110 (MQ=37)
                                           aGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATc                                                                                                    >  2:318146/1‑149 (MQ=255)
                                            gATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCATCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTTGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCAATAATGAATACAAGCAGCCAACATCg                                                                                                   >  5:286210/1‑149 (MQ=255)
                                            gATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCg                                                                                                   >  5:200997/1‑149 (MQ=255)
                                                  tCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTc                                                                                                <  1:331902/146‑1 (MQ=255)
                                                  tCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAg                                                                                             >  4:20776/1‑149 (MQ=255)
                                                     ccccGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGccc                                                                                          >  3:119108/1‑149 (MQ=255)
                                                       ccGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCg                                                                                         >  3:16/1‑148 (MQ=255)
                                                             gtggtgACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACCTCGCTCCAGCCCGcatccat                                                                                  <  6:111593/149‑1 (MQ=255)
                                                              tggtgACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGcatccatc                                                                                 <  6:292117/149‑1 (MQ=255)
                                                              tggtgACCAGGCCAGATTCAGAATACACATAGCTGTTTTCAGTTTTTTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGcatccatc                                                                                 <  6:297835/149‑1 (MQ=255)
                                                                gtgACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCgg                                                                               >  5:97874/1‑149 (MQ=255)
                                                                   aCCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGca                                                                             <  6:173205/148‑1 (MQ=255)
                                                                                     tACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGCCCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCg                                                           >  5:385729/1‑148 (MQ=255)
                                                                                           tAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTc                                                    <  1:410799/149‑1 (MQ=255)
                                                                                           tAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTc                                                    >  2:410799/1‑149 (MQ=255)
                                                                                             gCTGTTTTCTGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTcc                                                   <  6:184942/148‑1 (MQ=255)
                                                                                             gCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCa                                                  <  3:20776/149‑1 (MQ=255)
                                                                                             gCTGGTTTCAGTTTTTTGATTAATCACATCGCCGTGTCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGTCACCACAACAGCATCTGCCGTACGTTcc                                                   <  5:160011/148‑1 (MQ=255)
                                                                                              cTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCAt                                                 >  7:273534/1‑149 (MQ=255)
                                                                                              cTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCAt                                                 <  8:279746/149‑1 (MQ=255)
                                                                                               tGTTTTCAGTTTTGTGATTAATGACATCGCCGTGGCAAACGTTGCCCTCGGGGGTGTGGGCGGTGATCTCCCATTATGAAGACCAGGAGCGAACATCGCTCCAGCCCGCATCCATCGGCACCACAGCAGCATCTGCCGTACGTTACATg                                                >  8:137785/1‑149 (MQ=255)
                                                                                                gTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGa                                               >  7:330536/1‑149 (MQ=255)
                                                                                                   ttCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGAcc                                             <  6:312176/148‑1 (MQ=255)
                                                                                                   ttCAGTTTTGGGATTAATCACATCGCCTTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGCTCTCCCATAATGAAGACCAGGAGCCCACATCGCTCCAGCCCGCATCCCTCGGCCCCACAACCGCAGCTGCCGTACGTTCCCTTACcg                                            >  4:79750/1‑149 (MQ=255)
                                                                                                      aGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGt                                         <  3:180542/149‑1 (MQ=255)
                                                                                                       gTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTc                                                                                                >  5:240050/1‑93 (MQ=255)
                                                                                                       gTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTc                                                                                                <  6:240050/93‑1 (MQ=255)
                                                                                                       gTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCAt                                                                 >  2:272396/1‑124 (MQ=255)
                                                                                                        ttttGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCACCATCGCTCCAGCCCGCATCCATCGGCACGCCAACAGCATCTGCCGTACGTTCCATGACCGCGTaa                                       >  6:305872/1‑149 (MQ=255)
                                                                                                        ttttGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTaa                                       >  5:349771/1‑149 (MQ=255)
                                                                                                          ttGTGATTAATCACATCGCCGTGGCAAAGGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAAt                                      <  1:318146/148‑1 (MQ=255)
                                                                                                           tgtgATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATc                                     <  7:155765/148‑1 (MQ=255)
                                                                                                             tgATTAATCACATCTCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACGACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCa                                   <  4:16/148‑1 (MQ=255)
                                                                                                             tgATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCAc                                  >  8:386596/1‑149 (MQ=255)
                                                                                                              gATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCCCAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCAcc                                 >  5:9676/1‑149 (MQ=255)
                                                                                                               aTTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCAcc                                 <  6:349771/148‑1 (MQ=255)
                                                                                                               aTTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCAcc                                 >  5:371396/1‑148 (MQ=255)
                                                                                                                    tCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGccc                                                                                          >  2:119235/1‑86 (MQ=255)
                                                                                                                      acaTCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTc                         >  5:291700/1‑149 (MQ=255)
                                                                                                                      acaTCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTc                         <  3:278708/149‑1 (MQ=255)
                                                                                                                        aTCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCg                       >  6:80131/1‑149 (MQ=255)
                                                                                                                        aTCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCg                       >  7:79676/1‑149 (MQ=255)
                                                                                                                        aTCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCg                       <  7:386596/149‑1 (MQ=255)
                                                                                                                         tCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGCCTCTTCCgg                      >  6:390439/1‑149 (MQ=255)
                                                                                                                          cGCCGAGGGCAACGTTTGCCACGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCggg                     >  5:320897/1‑149 (MQ=255)
                                                                                                                             cGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGAGCAGGAGCAAACATCGCTCCAGCCCTCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAg                  <  3:200160/149‑1 (MQ=255)
                                                                                                                                 gCAAACGTTGCCCTCGGCGTTGTGGGAGCTGATATCACATAATTAAGACCAGGAGCAAACATCGCTCCAGACCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCga              <  6:385729/149‑1 (MQ=255)
                                                                                                                                 gCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATTAAGACCAGGAGACAACATCGCTCCAGCCCGCATCCATCGGAAACTCATCAGCATCTGCCGTTCGTTGCATTACCGCGTTATCCACCGACTCTTCCGGGCAGGCg               <  6:286210/148‑1 (MQ=255)
                                                                                                                                   aaaCGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCAc                                  >  3:202902/1‑127 (MQ=255)
                                                                                                                                   aaaCGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCAc                                  <  4:202902/127‑1 (MQ=255)
                                                                                                                                      cGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAAc         <  6:291700/149‑1 (MQ=255)
                                                                                                                                        ttGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATGCACCGACTCTTCCCGGCAGGCGAGAAACGc       <  6:200997/149‑1 (MQ=255)
                                                                                                                                        ttGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGc       >  3:191089/1‑149 (MQ=255)
                                                                                                                                           cccTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGctt     <  3:263605/148‑1 (MQ=255)
                                                                                                                                             cTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGcttctt  >  3:14125/1‑149 (MQ=255)
                                                                                                                                              tCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACAAGGAGCCAACATAGCTCCAGCCCGCATCCATCTGCACAACAACAGCATCTGCCGTACGTTCCATGACAGCGTAATCCACCGACTCTTCCGGGCAGGCGAAAAACGcttctt  >  2:165857/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
ACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGCTTCTT  >  NC_000913/2123495‑2123784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: