Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 360,234 G→T 5.4% M81I (ATG→ATT cynX → putative cyanate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913360,2340GT 5.4% 247.4 / 3.3 74M81I (ATG→ATTcynXputative cyanate transporter
Reads supporting (aligned to +/- strand):  ref base G (32/38);  new base T (2/2);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

GCTTTAGCGTGGCTGCCCTGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGT  >  NC_000913/360089‑360376
                                                                                                                                                 |                                                                                                                                              
gCTTTAGCGTGGCTGCCCTGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCTCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCTCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGt                                                                                                                                             <  6:311236/149‑1 (MQ=255)
gCTTTAGCGTGGCTGCCCTGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGt                                                                                                                                             >  5:343940/1‑149 (MQ=255)
  tttAGCGTGGCTGCCCTGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGa                                                                                                                                           <  1:128265/149‑1 (MQ=255)
          ggCTGCCCTGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTAcc                                                                                                                                   >  2:152219/1‑149 (MQ=255)
           gCTGCCCTGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTAccc                                                                                                                                  <  3:240641/149‑1 (MQ=255)
                ccTGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCaaa                                                                                                                             <  8:169397/149‑1 (MQ=255)
                  tGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGt                                                                                                                           >  6:270836/1‑149 (MQ=255)
                  tGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGt                                                                                                                           >  1:404057/1‑149 (MQ=255)
                    ttGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTgc                                                                                                                         <  8:126194/149‑1 (MQ=255)
                      gACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTgcgc                                                                                                                       >  7:140097/1‑149 (MQ=255)
                         cGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCgctgc                                                                                                                    >  2:158095/1‑149 (MQ=255)
                            tctGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCgctgctgc                                                                                                                 >  3:62295/1‑149 (MQ=255)
                            tctGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCgctgctgc                                                                                                                 >  8:90442/1‑149 (MQ=255)
                                ccGGTGGTTACCATGGGCGGGCTGGCGCTGGCCTGAAGCTGGCTTCATCAGCATGTCAGCTAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCtt                                                                                                               <  8:174025/147‑1 (MQ=255)
                                ccGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTa                                                                                                              <  7:219344/148‑1 (MQ=255)
                                  ggtggtTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTag                                                                                                             >  1:163733/1‑147 (MQ=255)
                                     ggtTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAgcg                                                                                                        >  3:123910/1‑149 (MQ=255)
                                       ttACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCa                                                                                                      <  5:270836/149‑1 (MQ=255)
                                         aCCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACt                                                                                                    >  2:15074/1‑149 (MQ=255)
                                           cATGGGCGAGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGc                                                                                                  >  8:81134/1‑149 (MQ=255)
                                               ggcgggcTGGCGCTGGCCGGATGCGGGCTTCATCAGCAGGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTCCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTAAGCAGCAGCGCACtttgcgg                                                                                              <  6:164983/149‑7 (MQ=255)
                                                 cgggcTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGATGGTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTtggtg                                                                                            <  5:146060/149‑1 (MQ=255)
                                                   ggcTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCTCCATCAGTCTGTTGCTGATTGCCGTCGGGGCATTGATGCGTGTGCTTTACCCGCAACGTGCGCTGCGGCTTAGCAGCGCACTGCTTggtggg                                                                                          <  8:376922/149‑1 (MQ=255)
                                                      tggcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATTCGTGAGCTTTACCCGCAAAGTgcgc                                                                                                                       <  4:310590/117‑1 (MQ=255)
                                                      tggcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATTCGTGAGCTTTACCCGCAAAGTgcgc                                                                                                                       <  4:315934/117‑1 (MQ=255)
                                                      tggcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATTCGTGAGCTTTACCCGCAAAGTgcgc                                                                                                                       >  3:310590/1‑117 (MQ=255)
                                                      tggcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATTCGTGAGCTTAACCCGCAAAGTgcgc                                                                                                                       >  3:315934/1‑117 (MQ=255)
                                                       ggcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGGCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTggtggggtgg                                                                                      <  7:11207/149‑1 (MQ=255)
                                                        gcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTggtggggtg                                                                                       <  2:182014/147‑1 (MQ=255)
                                                        gcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGgtggggtggg                                                                                     <  3:172559/149‑1 (MQ=255)
                                                          gctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGa                                                                                   >  3:330556/1‑149 (MQ=255)
                                                              gcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCgg                                                                               >  7:366418/1‑149 (MQ=255)
                                                               ccGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGc                                                                              <  6:343940/149‑1 (MQ=255)
                                                                 ggAAGCTGGGTTCATCAGCATGTGAGCGTACTTCGCAGTGTCGACATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGcat                                                                            <  8:351456/149‑1 (MQ=255)
                                                                       tGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCAtt                                                                        >  8:36954/1‑147 (MQ=255)
                                                                         gCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGGCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCa                                                                      <  7:81134/147‑1 (MQ=255)
                                                                           ttCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCg                                                                  >  3:380594/1‑149 (MQ=255)
                                                                               tcaGCATGTCAGCGACCGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGa                                                              >  6:91636/1‑149 (MQ=255)
                                                                               tcaGCATGTCAGCGAACGTCGCATTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGGACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGa                                                              <  2:404057/149‑1 (MQ=255)
                                                                                caGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGAt                                                             >  3:90433/1‑149 (MQ=255)
                                                                                   cATGTCAGCGAACGGCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGcc                                                          <  2:327620/149‑1 (MQ=255)
                                                                                      gTCCGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCGTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTGGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTc                                                       >  7:287017/1‑149 (MQ=255)
                                                                                       tCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCg                                                      <  7:36954/149‑1 (MQ=255)
                                                                                         aGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGt                                                    >  1:359488/1‑149 (MQ=255)
                                                                                         aGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGt                                                    <  7:90442/149‑1 (MQ=255)
                                                                                           cGAACGTCGCAGTGTCTCCATCAGTCTGTGGCTGATTGCCGTCGGTGCTTTGATGCGTGAGCTTTACCCGCAACGTGCGCTGCTGCTTAGCAGCGCAGTGGTTGGTGGGGTGGGGATCGGCATGATTCAGGCGGTGATGCCTTCGGTGa                                                  <  1:388276/149‑1 (MQ=255)
                                                                                           cGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGa                                                  <  8:140097/149‑1 (MQ=255)
                                                                                           cGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTACCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGa                                                  >  7:161941/1‑149 (MQ=255)
                                                                                                gTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCtt                                                        <  4:181551/138‑1 (MQ=255)
                                                                                                gTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCtt                                                        >  3:181551/1‑138 (MQ=255)
                                                                                                gTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTaaa                                             <  4:380594/149‑1 (MQ=255)
                                                                                                gTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTaaa                                             <  1:158095/149‑1 (MQ=255)
                                                                                                 tCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAAc                                            <  4:123910/149‑1 (MQ=255)
                                                                                                   gCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGa                                                  <  5:238335/141‑1 (MQ=255)
                                                                                                   gCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGa                                                  >  6:238335/1‑141 (MQ=255)
                                                                                                    cAGTCTCGCCATACGGCTGTTGCTGACTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTTGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAAcggc                                         <  2:349626/149‑1 (MQ=255)
                                                                                                     aGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGGGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAAcggcg                                        <  2:162412/149‑1 (MQ=255)
                                                                                                          cGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGttt                                    <  3:263051/148‑1 (MQ=255)
                                                                                                            ccATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTc                                   <  6:204809/147‑1 (MQ=255)
                                                                                                            ccATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTc                                   >  5:204809/1‑147 (MQ=255)
                                                                                                             cATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcagc                                <  2:18314/149‑1 (MQ=255)
                                                                                                             cATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcag                                 >  1:18314/1‑148 (MQ=255)
                                                                                                              aTCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcag                                 >  1:393334/1‑147 (MQ=255)
                                                                                                                          cTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGTCGGTTTCAGCAGCGCACGCCACt                   <  4:62295/149‑1 (MQ=255)
                                                                                                                          cTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACt                   >  2:161475/1‑149 (MQ=255)
                                                                                                                          cTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACt                   <  1:152219/149‑1 (MQ=255)
                                                                                                                          cTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGGGATTAAACGGCGGTTTCAGCAGCGCACGCCACt                   >  7:300185/1‑149 (MQ=255)
                                                                                                                             aTTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTg                  <  2:163733/147‑1 (MQ=255)
                                                                                                                              ttGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGt                >  4:230408/1‑148 (MQ=255)
                                                                                                                                gCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGa              <  3:230408/148‑1 (MQ=255)
                                                                                                                                 ccGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGAt             <  5:387536/148‑1 (MQ=255)
                                                                                                                                 ccGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATg            >  2:92956/1‑149 (MQ=255)
                                                                                                                                         gCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCtgtg    >  2:419484/1‑149 (MQ=255)
                                                                                                                                           aTTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGt  >  7:354860/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GCTTTAGCGTGGCTGCCCTGTTGACCGCTCTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGT  >  NC_000913/360089‑360376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: