Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,705,868 A→T 5.3% I419I (ATT→ATA lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,705,8680AT 5.3% 294.3 / 3.7 92I419I (ATT→ATAlepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base A (45/42);  new base T (3/2);  total (48/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTA  >  NC_000913/2705721‑2706016
                                                                                                                                                   |                                                                                                                                                    
cAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGATGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGta                                                                                                                                                      <  6:181280/148‑1 (MQ=255)
 agagACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGtaa                                                                                                                                                     >  8:215831/1‑148 (MQ=255)
 agagACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGtaa                                                                                                                                                     >  6:17702/1‑148 (MQ=255)
   agaCGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGtaataa                                                                                                                                                  <  2:113802/149‑1 (MQ=255)
     aCGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACg                                                                                                                                                >  7:77244/1‑149 (MQ=255)
       gcttAACCACGCGAGGTAGATTTCAGGCGATCGAAGAATTCGAGCTCCACTTCAGCCATCGGGATCTCTTACTTCTGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGt                                                                                                                                               <  4:240294/145‑1 (MQ=255)
       gCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGtt                                                                                                                                              >  3:186189/1‑149 (MQ=255)
       gCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGAACAACGTAATAACGt                                                                                                                                               >  3:240294/1‑148 (MQ=255)
          tAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGcc                                                                                                                                           >  5:285387/1‑149 (MQ=255)
              cACGCGAGGTACATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCgaga                                                                                                                                       <  7:351503/149‑1 (MQ=255)
                cgcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTTATTACGTTgtgc                                                                                                                                          <  3:305425/144‑4 (MQ=255)
                cgcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTTATTACGTTgtgc                                                                                                                                          >  4:305425/1‑141 (MQ=255)
                cgcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGata                                                                                                                                     >  5:26885/1‑149 (MQ=255)
                cgcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGata                                                                                                                                     <  8:93128/149‑1 (MQ=255)
                  cgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGata                                                                                                                                     >  4:267207/1‑147 (MQ=255)
                  cgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGata                                                                                                                                     <  3:267207/147‑1 (MQ=255)
                   gAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGc                                                                                                                                  <  5:17702/149‑1 (MQ=255)
                     ggTAGATTTCAGGCGCTCGAAGAAGTCGAGCACCACATCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCt                                                                                                                                <  2:298888/149‑1 (MQ=255)
                     ggTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGCTCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCt                                                                                                                                <  2:278621/149‑1 (MQ=255)
                      gTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACCTTGCCGAGATATGCCTg                                                                                                                               >  8:309929/1‑149 (MQ=255)
                        aGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCg                                                                                                                             >  4:154764/1‑149 (MQ=255)
                        aGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCg                                                                                                                             >  4:156509/1‑149 (MQ=255)
                           tttCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCgg                                                                                                                            >  8:159526/1‑147 (MQ=255)
                           tttCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgca                                                                                                                          <  6:191012/149‑1 (MQ=255)
                             tCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGTGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgca                                                                                                                          >  6:321793/1‑147 (MQ=255)
                             tCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGTGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgca                                                                                                                          <  5:321793/147‑1 (MQ=255)
                               aGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCAACTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGAGTGTTTCTACGCACAACGTAATAACGTTGCCGAGATCTGCCTGCGgcagcag                                                                                                                      >  6:315009/1‑149 (MQ=255)
                                    aTCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCAtgt                                                                                                                 <  8:52770/149‑1 (MQ=255)
                                       gaagaaGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagcagca                                                                                                                    >  8:260514/1‑143 (MQ=255)
                                       gaagaaGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagcagca                                                                                                                    <  7:260514/143‑1 (MQ=255)
                                       gaagaaGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGa                                                                                                               <  1:324352/148‑1 (MQ=255)
                                        aagaagTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGaca                                                                                                             >  6:60946/1‑149 (MQ=255)
                                        aagaagTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGaca                                                                                                             >  8:295321/1‑149 (MQ=255)
                                           aagTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACActc                                                                                                          <  7:237677/149‑1 (MQ=255)
                                                  gCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATc                                                                                                   <  4:186189/149‑1 (MQ=255)
                                                      cacTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGGAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGc                                                                                                >  4:213820/1‑148 (MQ=255)
                                                      cacGTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGGAATAACGGTGCCGAGATATCCCTGCGGCAGCAGCATGAGCCACTCGGCAATCGGCt                                                                                               >  5:73230/1‑149 (MQ=255)
                                                       acTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTc                                                                                              <  7:215831/149‑1 (MQ=255)
                                                         ttCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTcgc                                                                                            >  7:52832/1‑149 (MQ=255)
                                                         ttCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTcgc                                                                                            >  4:54971/1‑149 (MQ=255)
                                                             gCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAg                                                                                        >  2:22856/1‑149 (MQ=255)
                                                                aTCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTc                                                                                     <  8:52832/149‑1 (MQ=255)
                                                                   gggATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTa                                                                                  <  5:154339/149‑1 (MQ=255)
                                                                     gATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTc                                                                                              >  1:360219/1‑135 (MQ=255)
                                                                     gATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTc                                                                                              <  2:360219/135‑1 (MQ=255)
                                                                       tctcGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATg                                                                              >  6:236627/1‑149 (MQ=255)
                                                                         tcGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGt                                                                             >  7:141440/1‑148 (MQ=255)
                                                                         tcGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGt                                                                             >  1:317654/1‑148 (MQ=255)
                                                                             aCGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGcc                                                                                                                                           >  8:8745/1‑82 (MQ=255)
                                                                             aCGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGcc                                                                                                                                           <  7:8745/82‑1 (MQ=255)
                                                                             aCGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTAtt                                                                         >  3:363278/1‑148 (MQ=255)
                                                                              cggcAGCGCCACCTGAATACCGTGGTAAACCATATAGGGCTGCCCGCCGCGTTTTGCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTa                                                                       <  2:268342/146‑1 (MQ=255)
                                                                              cGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTa                                                                       <  6:285387/149‑1 (MQ=255)
                                                                               gTCCGCGCCACCTGATTACCGTGGTAAACCATATTGGTATGCACGCCGCGTTTTGCTACGCAAAACGTATTAACGTTTCCGAGATCTGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTAc                                                                      >  7:287861/1‑149 (MQ=255)
                                                                                tCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTAcc                                                                     >  4:365420/1‑149 (MQ=255)
                                                                                     gcCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcagg                                                                >  6:302262/1‑149 (MQ=255)
                                                                                     gcCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcagg                                                                <  7:295321/149‑1 (MQ=255)
                                                                                       cACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcagg                                                                >  5:402365/1‑147 (MQ=255)
                                                                                              ttACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGa                                                       <  5:401478/149‑1 (MQ=255)
                                                                                                aCCGTGGTAAACCATATTGGTCTGCACGCCTCGTTTTTCTACGCACAACGTAATAACGTTTCCGAGATATGCCTGCTGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGTCAGCTTGGAt                                                      <  5:315009/148‑1 (MQ=255)
                                                                                                 ccGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATgg                                                    >  6:360910/1‑149 (MQ=255)
                                                                                                  cGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCAGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATggg                                                   >  5:218694/1‑149 (MQ=255)
                                                                                                    tGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCt                                                 >  5:192190/1‑149 (MQ=255)
                                                                                                       tAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCTGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTc                                              <  7:309929/149‑1 (MQ=255)
                                                                                                        aaaCCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTc                                              >  1:205428/1‑148 (MQ=255)
                                                                                                         aaCCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGa                                            <  3:365420/149‑1 (MQ=255)
                                                                                                         aaCCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGa                                            >  2:74790/1‑149 (MQ=255)
                                                                                                          aCCATATTGGTCTGCACGCCTCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGAc                                           <  6:73230/149‑1 (MQ=255)
                                                                                                          aCCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAAGGTTGCGGAGTTATGCCTGCGGCAGCGGCATGTGCCACTCTGCTATAGGCTGGCGCAGTTCGTAGATGTTATTTAGCGCTGGGAGCATGGATGGGGTGTCGag                                           >  2:260514/1‑148 (MQ=255)
                                                                                                            cATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGGCACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGt                                         <  3:156509/149‑1 (MQ=255)
                                                                                                            cATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACg                                          >  3:283181/1‑148 (MQ=255)
                                                                                                             atatTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTa                                        <  7:159526/149‑1 (MQ=255)
                                                                                                             atatTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTa                                        <  6:192190/149‑1 (MQ=255)
                                                                                                              tatTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGt                                               >  8:315028/1‑141 (MQ=255)
                                                                                                              tatTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGt                                               <  7:315028/141‑1 (MQ=255)
                                                                                                              tatTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACg                                          >  2:248738/1‑146 (MQ=255)
                                                                                                              tatTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACg                                          >  3:241590/1‑146 (MQ=255)
                                                                                                                   gTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATaa                                   <  8:77244/148‑1 (MQ=255)
                                                                                                                      tGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTGATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTtc                               >  4:307056/1‑149 (MQ=255)
                                                                                                                       gCACGCCGCGTTTTTCTACGCACAACGTTATAACGTTGCCGAGATATGCCTGCCGCAGAAGCATGTGAAAATATGCATTCGGCTCGCGCAGTTCGGAGATGTTATTAACCGCAGGCAGCTTGGCTGGGCTGTCGACGTAGATAACTtct                              >  6:192521/1‑149 (MQ=255)
                                                                                                                           gccgcGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTg                          <  5:60946/149‑1 (MQ=255)
                                                                                                                            ccgcGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGa                         <  6:218694/149‑1 (MQ=255)
                                                                                                                             cgcgTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCt                                                                                               <  4:66770/78‑1 (MQ=255)
                                                                                                                             cgcgTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCt                                                                                               >  3:66770/1‑78 (MQ=255)
                                                                                                                             cgcgTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGAc                        <  1:22856/149‑1 (MQ=255)
                                                                                                                                       tACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCaa              >  7:347838/1‑149 (MQ=255)
                                                                                                                                         cGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAAc             <  8:141440/148‑1 (MQ=255)
                                                                                                                                         cGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTAATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACt            <  3:307056/149‑1 (MQ=255)
                                                                                                                                          gCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTtctc                             <  5:310220/131‑1 (MQ=255)
                                                                                                                                          gCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTtctc                             >  6:310220/1‑131 (MQ=255)
                                                                                                                                          gCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACtt           <  3:54971/149‑1 (MQ=255)
                                                                                                                                           ggcaACGTTATTACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcagg                                                                <  5:127/93‑1 (MQ=255)
                                                                                                                                           ggcaACGTTATTACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcagg                                                                >  6:127/3‑95 (MQ=255)
                                                                                                                                            acaACGTAATAACGTTGCCGAGATATGCCTGCCCCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGAAGGGCGGTCGACGAAGATAACATCTCTTGACATCGTATCAACGTCa         >  1:278645/1‑149 (MQ=255)
                                                                                                                                              aaCGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTGCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTGTCAACTTCATa       <  8:287861/149‑1 (MQ=255)
                                                                                                                                                cGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATaca     <  6:402365/149‑1 (MQ=255)
                                                                                                                                                  taataaCGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATa       <  4:338933/145‑1 (MQ=255)
                                                                                                                                                  taataaCGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATa       >  3:338933/1‑145 (MQ=255)
                                                                                                                                                   aataaCGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTa  >  1:252951/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTA  >  NC_000913/2705721‑2706016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: