Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,708,427 G→A 7.1% intergenic (‑722/+189) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,708,4270GA 7.1% 304.7 / 8.7 98intergenic (‑722/+189)dppA/proKdipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base G (38/53);  new base A (3/4);  total (41/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

ATAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCT  >  NC_000913/3708281‑3708572
                                                                                                                                                  |                                                                                                                                                 
aTAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGtt                                                                                                                                                 <  5:186806/149‑1 (MQ=255)
aTAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGtt                                                                                                                                                 >  2:118263/1‑149 (MQ=255)
   aGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGa                                                                                                                                               <  6:238622/148‑1 (MQ=255)
    ggCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGc                                                                                                                                             <  1:299248/149‑1 (MQ=255)
      cGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAg                                                                                                                                           >  5:382837/1‑149 (MQ=255)
            cgccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCtttt                                                                                                                                     <  3:220478/149‑1 (MQ=255)
            cgccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCtttt                                                                                                                                     <  1:135684/149‑1 (MQ=255)
            cgccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCtttt                                                                                                                                     <  2:273989/149‑1 (MQ=255)
             gccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTc                                                                                                                                    >  8:205060/1‑149 (MQ=255)
              ccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTc                                                                                                                                    >  1:378799/1‑148 (MQ=255)
               cgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCg                                                                                                                                   >  6:60543/1‑148 (MQ=255)
                 cATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAt                                                                                                                                <  6:382837/149‑1 (MQ=255)
                  aTCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAtt                                                                                                                               <  7:56815/149‑1 (MQ=255)
                   tCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAttt                                                                                                                              >  8:367984/1‑149 (MQ=255)
                      gACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGCGCTGCAGCTGCAAAAAAGCCTGCTCGTGGCGCAGGCGTTTCGAATTTGGt                                                                                                                           >  1:75224/1‑149 (MQ=21)
                      gACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGt                                                                                                                           <  2:378799/149‑1 (MQ=255)
                         aaTGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGTACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCgg                                                                                                                        <  2:373439/149‑1 (MQ=255)
                             gCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGAt                                                                                                                    <  6:320407/149‑1 (MQ=255)
                                ggCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATa                                                                                                                   >  3:5508/1‑147 (MQ=255)
                                   acaTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGa                                                                                                              <  2:160823/149‑1 (MQ=255)
                                      tttGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTa                                                                                                           <  5:266285/149‑1 (MQ=255)
                                      tttGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTa                                                                                                           <  5:260556/149‑1 (MQ=255)
                                         gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACt                                                                                                         <  5:60543/148‑1 (MQ=255)
                                          ccTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGGCGGTGATAGAGGATTACTc                                                                                                        <  7:413183/148‑1 (MQ=255)
                                           cTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTAGc                                                                                                      >  1:205006/1‑149 (MQ=255)
                                             gATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCa                                                                                                    >  4:333247/1‑149 (MQ=255)
                                              aTGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCACATGAGCTGCAGATGCAAAAAAGCCTGCTCGATCAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCAc                                                                                                   >  1:13366/1‑149 (MQ=255)
                                                  gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCg                                                                                               <  6:169362/149‑1 (MQ=255)
                                                   aCGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGt                                                                                              <  1:118263/149‑1 (MQ=255)
                                                   aCGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGt                                                                                              <  1:56615/149‑1 (MQ=255)
                                                    cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTg                                                                                             <  6:69083/149‑1 (MQ=255)
                                                     gaggACGCGGCTTATCATGCCTAAGTAGAGGCGAACCTCCCTGCCCCCGTAGGTTCTCATCCTCATGAGCTGCAGAGGCAAAAAAGCCTGCGCGTTGAGCAGGCTTTTCGAATTTTGGCGGTGATAGAGGATTACTCGCCACTTCTTgg                                                                                            <  8:288472/146‑1 (MQ=17)
                                                     gCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTgg                                                                                            <  8:245883/149‑1 (MQ=255)
                                                       tGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCt                                                                                          >  5:346459/1‑149 (MQ=255)
                                                       tGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCt                                                                                          >  4:362947/1‑149 (MQ=255)
                                                        gACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTAATCGCCCCtgcctgaa                                                                                           >  5:330221/1‑140 (MQ=255)
                                                                ttATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCg                                                                                 >  7:347376/1‑149 (MQ=255)
                                                                 tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCa                                                                                                                                            >  1:122509/1‑89 (MQ=255)
                                                                 tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCa                                                                                                                                            <  2:122509/89‑1 (MQ=255)
                                                                  aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCtttttt                                                                                                                                   <  8:106569/97‑3 (MQ=14)
                                                                  aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCtttttt                                                                                                                                   >  7:106569/1‑95 (MQ=14)
                                                                  aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGAATATGCAAAAAAGCATGCACGTTGATAAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCATGCggg                                                                               >  7:100219/1‑149 (MQ=18)
                                                                      ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTc                                                                                                        >  8:299376/1‑120 (MQ=255)
                                                                      ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTc                                                                                                        <  7:299376/120‑1 (MQ=255)
                                                                      ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGt                                                                           <  7:141759/149‑1 (MQ=255)
                                                                      ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGt                                                                           <  7:297649/149‑1 (MQ=255)
                                                                      ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATAATCAAGAGCTGGAGATGCAAAAAAGCATGCTCGTTGAGCATGAATTTCGAATGTGGTAGGTGATAGAGAAATACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGt                                                                           >  2:269331/1‑149 (MQ=18)
                                                                         cTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGc                                                                        <  2:59142/149‑1 (MQ=255)
                                                                         cTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGc                                                                        <  6:346459/149‑1 (MQ=255)
                                                                          tACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGCGCTGCAGATGCAAAAAAGCCTGCGCGTAGAGCAGGCTTGTCGAATTTGGTCGGGGATAGAGGATTAATCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCt                                                                       >  7:298407/1‑149 (MQ=18)
                                                                            cGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCt                                                                       <  3:187817/147‑1 (MQ=255)
                                                                               cgagtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgcgc                                                                  <  6:185386/149‑1 (MQ=255)
                                                                                  gtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAAc                                                               <  6:227722/149‑1 (MQ=255)
                                                                                  gtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAAc                                                               >  5:227722/1‑149 (MQ=255)
                                                                                   tcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCg                                                                                               >  8:258536/1‑116 (MQ=255)
                                                                                   tcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCg                                                                                               <  7:258536/116‑1 (MQ=255)
                                                                                   tcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgcg                                                                   <  7:125462/144‑1 (MQ=255)
                                                                                   tcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgcg                                                                   >  8:125462/1‑144 (MQ=255)
                                                                                   tcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACg                                                              >  7:336715/1‑149 (MQ=255)
                                                                                   tcgaACCCCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACg                                                              >  7:211971/1‑149 (MQ=255)
                                                                                    cgaACCTCCCTGCCCCCGGAGGTTCTCCTCCGCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCCGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGt                                                             >  3:283956/1‑149 (MQ=255)
                                                                                        ccTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtctc                                                         <  4:354329/149‑1 (MQ=255)
                                                                                         cTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtctct                                                        <  8:347376/149‑1 (MQ=255)
                                                                                            ccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgc                                                     <  8:336715/149‑1 (MQ=255)
                                                                                             cTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgct                                                    <  7:367984/149‑1 (MQ=255)
                                                                                                  cccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgcttcgct                                               <  8:211971/149‑1 (MQ=255)
                                                                                                   ccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTc                                              <  7:205060/149‑1 (MQ=255)
                                                                                                     ggAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGa                                            <  4:5508/149‑1 (MQ=255)
                                                                                                       aGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGt                                          <  3:333247/149‑1 (MQ=255)
                                                                                                         gTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCg                                        <  3:362947/149‑1 (MQ=255)
                                                                                                               aTCCTCATGAGCTGCAGATGAAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAAc                                     <  8:157245/146‑1 (MQ=255)
                                                                                                                   tCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgc                                                                    >  7:253099/1‑111 (MQ=255)
                                                                                                                   tCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgc                                                                    <  8:253099/111‑1 (MQ=255)
                                                                                                                     aTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgctt                                                   >  7:143625/1‑126 (MQ=255)
                                                                                                                     aTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgctt                                                   <  8:143625/126‑1 (MQ=255)
                                                                                                                            gCAGATGCAAAACAGCCTGCGCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATATATGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCGCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAg                     <  4:283956/149‑1 (MQ=38)
                                                                                                                            gCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAg                     <  1:269331/149‑1 (MQ=255)
                                                                                                                              aGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCAGTTGCTGGCGCAAc                                                               <  1:365987/105‑1 (MQ=255)
                                                                                                                              aGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCAGTTGCTGGCGCAAc                                                               >  2:365987/1‑105 (MQ=255)
                                                                                                                                 tGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAg                                           <  5:151025/122‑1 (MQ=255)
                                                                                                                                 tGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAg                                           >  6:151025/1‑122 (MQ=255)
                                                                                                                                 tGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtct                >  5:407322/1‑149 (MQ=255)
                                                                                                                                 tGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtct                >  3:202562/1‑149 (MQ=255)
                                                                                                                                  gCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtct                >  2:128921/1‑148 (MQ=255)
                                                                                                                                  gCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtct                >  2:134688/1‑148 (MQ=255)
                                                                                                                                   cAAAAAAGCCTGCTCGGTGAGCAGGCTTTTCGATTTTGGTCGGTGATTGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGGTGGTGGCGCATCGTTCTCTGGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtct                <  2:75224/147‑1 (MQ=255)
                                                                                                                                     aaatatccTGCTCGTTGAGGAGGGTTTTCGAATTTGGTCTGTGATAGAGTATTTGTCGCCACTTCGTGGATCGCCCTTCGGGCCGGTGCTGGCGCATCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATc            <  2:13366/143‑1 (MQ=37)
                                                                                                                                     aaaccgCCTGCTCGTTGAGCAGGCTTTTCGATTTTTGTCGGTTAGAGAGGATTACTCGCCACTTCGTGGCTCTCGCTTCTGTTCGTTTCTCGCTCTACGTTCTCTCGCGTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATc            <  8:298407/144‑1 (MQ=25)
                                                                                                                                      aaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATcc           >  7:411964/1‑149 (MQ=255)
                                                                                                                                      aaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgc                                                                    <  7:301972/92‑1 (MQ=255)
                                                                                                                                      aaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgc                                                                    >  8:301972/1‑92 (MQ=255)
                                                                                                                                      aaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtc                 <  6:101723/143‑1 (MQ=255)
                                                                                                                                      aaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtc                 >  5:101723/1‑143 (MQ=255)
                                                                                                                                       caaGCCTCCTCGTTGAGCAGTTTTTTAGAATTTTGTGGGTGATAGAGGTTTACTCGCCTCTTCGTTGATCGATCTGCTGGCCGTTGCTGGCGCTACGTTCTCTCGCTTAGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCt          <  8:100219/148‑1 (MQ=25)
                                                                                                                                       aaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCt          >  5:219764/1‑149 (MQ=255)
                                                                                                                                         aGCCTGCTCAACGAGCAGTCTTTTCGTATTTGGTCGGTGATAGAGGATTACTCGCCACTTGGTGGCGCGCCCTGCGGGCCGTTGCTGGAGCAACGTGCTCTCGCTTCGCTCGTGTCGAACCTCCCTGCGCCCGGAGGTTCTCATCCTCa        <  8:123414/149‑1 (MQ=255)
                                                                                                                                            cTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGATCGCCCTGAGGGCCGTAGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCATGCCCCCGGAGGATCTCATCCTCATGa     >  7:356369/1‑149 (MQ=38)
                                                                                                                                               cTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGACGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTAATCCTCATGAGCt  >  3:358940/1‑149 (MQ=255)
                                                                                                                                                  gTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGa                                            <  7:168377/104‑1 (MQ=255)
                                                                                                                                                  gTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGa                                            >  8:168377/1‑104 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCT  >  NC_000913/3708281‑3708572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: