Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 655,361 A→G 5.2% I203T (ATC→ACC)  dcuC ← anaerobic C4‑dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913655,3610AG 5.2% 242.3 / 2.9 79I203T (ATC→ACC) dcuCanaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base A (37/37);  new base G (2/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

CGTGAACGGCAAAATGGCATAAAACGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGACTGATACCAACGT  >  NC_000913/655213‑655508
                                                                                                                                                    |                                                                                                                                                   
cGTGAACGGCAAAATAGCATAAAACGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTgaga                                                                                                                                                     <  7:321609/149‑1 (MQ=255)
  tGAACGGCAAAATGGCATAAAACGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTgaga                                                                                                                                                     >  7:8588/1‑147 (MQ=255)
      cGGCAAAATGGCATAAAACGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTTCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAgg                                                                                                                                                 >  6:324882/1‑147 (MQ=255)
       ggCAAAATGGCATAAAACGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAg                                                                                                                                              >  8:233537/1‑149 (MQ=255)
       ggCAAAATGGCATAAAACGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAg                                                                                                                                              >  8:136751/1‑149 (MQ=255)
           aaaTGGCATAAAACGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATTATTGCAGCAATTGAGATAGGCAGcgtc                                                                                                                                          >  3:20465/1‑149 (MQ=255)
                     aaaCGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAg                                                                                                                                 <  5:288743/148‑1 (MQ=255)
                     aaaCGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAg                                                                                                                                 <  8:209505/148‑1 (MQ=255)
                         gCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCg                                                                                                                              >  3:147693/1‑147 (MQ=255)
                         gCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGaa                                                                                                                            <  7:41840/149‑1 (MQ=255)
                             gAGCAGTGGTGGTGATTTCACTGCCATCTAACATTTCATGAGAGATGTGATATTTATTATCAATATAACGTTGCCAGAAGAAGTTGGAGATCGCAATGGCGATCATTGCGGCAATTGAGTTAGGCAGCGTCGTTTTGAAGGCGAAGTc                                                                                                                         >  7:240349/1‑148 (MQ=255)
                                cAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAAt                                                                                                                      >  2:96194/1‑148 (MQ=255)
                                   tggtggtgTTTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACCTTGCCAGAATAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGc                                                                                                                  <  2:332793/149‑1 (MQ=255)
                                   tggtggtgATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGc                                                                                                                  <  7:136751/149‑1 (MQ=255)
                                     gtggtgATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGa                                                                                                                <  4:147693/149‑1 (MQ=255)
                                      tggtgATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGa                                                                                                                >  5:233708/1‑148 (MQ=255)
                                       ggtgATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACa                                                                                                              >  7:78530/1‑149 (MQ=255)
                                        gtgATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGAAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACAt                                                                                                             >  4:213584/1‑149 (MQ=255)
                                          gATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACAttt                                                                                                           >  4:76336/1‑149 (MQ=255)
                                               cACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCgg                                                                                                        >  7:1549/1‑147 (MQ=255)
                                                aCTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGaa                                                                                                      <  3:76336/148‑1 (MQ=255)
                                                 cTGTCATCTAAGATTTCATGAGAGATGTGCTATTTTTTATCCAGATAACGTTGCCAGAAGCATTGGTCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCTGCGTCGTTTTGAAGGCGAAGGCAATCAGCGACATTTCGGAAGc                                                                                                    <  7:247143/149‑1 (MQ=255)
                                                    aCATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTg                                                                                                 <  1:209597/149‑1 (MQ=255)
                                                     cATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTgc                                                                                                <  3:178273/149‑1 (MQ=255)
                                                      aTCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGa                                                                                                                <  7:86744/132‑1 (MQ=255)
                                                      aTCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGa                                                                                                                >  8:86744/1‑132 (MQ=255)
                                                       tatAACATTTAATGAGAGATTTTCTCTTTTTTATACAGATAACGTTGACAGAATAAGTGGGCGATCGACATGCCGATAATTGCAGAAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGc                                                                                                                  <  2:252701/127‑1 (MQ=255)
                                                       tCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACa                                                                                                              <  8:92421/133‑1 (MQ=255)
                                                       tCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACa                                                                                                              >  7:92421/1‑133 (MQ=255)
                                                       tCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCt                                                                                                   <  2:102023/144‑1 (MQ=255)
                                                       tCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCt                                                                                                   >  1:102023/1‑144 (MQ=255)
                                                       tCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAAGTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTgcgc                                                                                              >  4:239288/1‑149 (MQ=255)
                                                       tCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGACGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTgcgc                                                                                              >  1:202134/1‑149 (MQ=255)
                                                       tCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGAGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGc                                                                                                                  >  1:252701/1‑129 (MQ=255)
                                                        cTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGt                                                                                                                          >  7:281196/1‑120 (MQ=255)
                                                        cTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGt                                                                                                                          <  8:281196/120‑1 (MQ=255)
                                                         tAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGcgccg                                                                                            >  8:182638/1‑149 (MQ=255)
                                                             aTTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAg                                                                                        >  6:318033/1‑149 (MQ=255)
                                                              tttCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAg                                                                                        >  8:69564/1‑148 (MQ=255)
                                                                tCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCACAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGCTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGca                                                                                      <  1:71640/148‑1 (MQ=255)
                                                                 cATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCGAGcac                                                                                     <  8:40475/148‑1 (MQ=255)
                                                                             gCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGGTAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTg                                                                          >  1:112929/1‑147 (MQ=255)
                                                                             gCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGGTAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTg                                                                          >  1:117671/1‑147 (MQ=255)
                                                                             gCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAATCTTGCGCCGCCAGCACcaca                                                                                 <  5:160157/140‑1 (MQ=255)
                                                                             gCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACcaca                                                                                 >  6:160157/1‑140 (MQ=255)
                                                                              ctctTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGGTAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGaa                                                                        <  2:112929/148‑1 (MQ=255)
                                                                              ctctTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGGTAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGaa                                                                        <  2:117671/148‑1 (MQ=255)
                                                                               tctTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGt                                                                      >  2:321925/1‑149 (MQ=255)
                                                                                 ttttttATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCg                                                                    >  5:232611/1‑149 (MQ=255)
                                                                                       aTCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGc                                                                >  1:343146/1‑147 (MQ=255)
                                                                                          cAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCgaga                                                            >  2:353531/1‑148 (MQ=255)
                                                                                          cAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGaa                                                           >  7:239198/1‑149 (MQ=255)
                                                                                          cAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGaa                                                           >  2:126912/1‑149 (MQ=255)
                                                                                              tAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGaataat                                                       <  7:69564/149‑1 (MQ=255)
                                                                                               aaCGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATc                                                      >  8:259940/1‑149 (MQ=255)
                                                                                                aCGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATc                                                      <  5:318033/148‑1 (MQ=255)
                                                                                                aCGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGGCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATcg                                                     <  4:46432/149‑1 (MQ=255)
                                                                                                 cGTTGCCAGAAGAAGTGGGTGATCGCCATTCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATcgc                                                    <  4:34743/149‑1 (MQ=255)
                                                                                                       cAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCgg                                              <  7:41981/149‑1 (MQ=255)
                                                                                                         gaagaaGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCgggg                                            <  8:1549/149‑1 (MQ=255)
                                                                                                          aagaagTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGa                                           <  1:96194/149‑1 (MQ=255)
                                                                                                             aagTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCTTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGc                                        <  6:233708/149‑1 (MQ=255)
                                                                                                               gTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATATGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACAATTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGaa                                                                        >  1:151442/1‑115 (MQ=255)
                                                                                                               gTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGaa                                                                        <  2:151442/115‑1 (MQ=255)
                                                                                                               gTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCga                                                              <  5:252803/125‑1 (MQ=255)
                                                                                                               gTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCga                                                              >  6:252803/1‑125 (MQ=255)
                                                                                                                  ggCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACaaa                                   <  7:228076/149‑1 (MQ=255)
                                                                                                                     gATCGCCATGCCGATAATTGCAGCAATGGAGATAGGCAGCGTCGTTTTGAAGGCGAAGGCAATCAGCGACATTGCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATgg                                <  1:256055/149‑1 (MQ=255)
                                                                                                                      aTCGCCATGCCGATAATTGCAGAAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGgc                               <  2:202134/149‑1 (MQ=255)
                                                                                                                       tCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATgg                                >  4:14131/1‑147 (MQ=255)
                                                                                                                            aTGCCGATAATTGCAGCAATTGAGAGAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCagcagc                         <  2:124107/149‑1 (MQ=255)
                                                                                                                             tGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCt                                                                           <  5:211511/98‑1 (MQ=255)
                                                                                                                             tGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCt                                                                           >  6:211511/1‑98 (MQ=255)
                                                                                                                             tGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCt                        >  7:3625/1‑149 (MQ=255)
                                                                                                                                      ttGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGa               <  5:324882/149‑1 (MQ=255)
                                                                                                                                        gcagcaATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGAc              <  1:353531/148‑1 (MQ=255)
                                                                                                                                        gcagcaATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGACt             >  4:150232/1‑149 (MQ=255)
                                                                                                                                         cagcaATTGAGATAGGCAGAGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATACCCTGAAGTCGGTGCGAGAATAATCGACGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGACt             >  1:267882/1‑148 (MQ=255)
                                                                                                                                             aaTTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGACTGATAc        <  7:182638/149‑1 (MQ=255)
                                                                                                                                              aTTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGTGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGACTGATAcc       <  6:155906/149‑1 (MQ=255)
                                                                                                                                               ttGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACCTCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGACTGATAcc       >  2:280560/1‑148 (MQ=255)
                                                                                                                                                 gagaTAGGCAGCGTAGTTTTGAAGGCGAAGTCAATCAGCGACATTTTGGAAGCGTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGACTGATACCaa     <  6:308470/148‑1 (MQ=255)
                                                                                                                                                   gaTAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACCTCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGACTGATACCAACGt  <  3:239288/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTGAACGGCAAAATGGCATAAAACGCAGGAGCAGTGGTGGTGATTTCACTGACATCTAACATTTCATGAGAGATGTGCTCTTTTTTATCCAGATAACGTTGCCAGAAGAAGTGGGCGATCGCCATGCCGATAATTGCAGCAATTGAGATAGGCAGCGTCGTTTTGAAGGCGAAGTCAATCAGCGACATTTCGGAAGCTTGCGCCGCCAGCACCACATCCCCTGAAGTCGGTGCGAGAATAATCGCCGCCGGGGAGGCACAAATGGCAGCAGCTGCGCCACGACTGATACCAACGT  >  NC_000913/655213‑655508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: