Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,521,690 T→C 5.4% D459G (GAT→GGT)  yncD ← putative iron outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,521,6900TC 5.4% 224.6 / 3.0 74D459G (GAT→GGT) yncDputative iron outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (30/39);  new base C (2/2);  total (32/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

CGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTT  >  NC_000913/1521543‑1521838
                                                                                                                                                   |                                                                                                                                                    
cgcgTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATc                                                                                                                                                     <  1:103964/149‑1 (MQ=255)
   gTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATAGTCGGCGTTTCAAAACCTCTCCCGGCTGCCAGATAGATATTCCAGGCATCGGt                                                                                                                                                  >  3:254216/1‑149 (MQ=255)
      ttACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATAACGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGGTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCa                                                                                                                                                <  7:146493/148‑1 (MQ=255)
            ccGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCata                                                                                                                                         <  5:216659/149‑1 (MQ=255)
               aTCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGTACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCa                                                                                                                                                >  4:189834/1‑139 (MQ=255)
               aTCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGTACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCa                                                                                                                                                <  3:189834/139‑1 (MQ=255)
               aTCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTAATTAATAGTCGGCGTTTCAAAACCTAGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATAttt                                                                                                                                      >  5:49479/1‑149 (MQ=255)
                tctcAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATAttt                                                                                                                                      >  2:301603/1‑148 (MQ=255)
                    aaTTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTATTCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTaa                                                                                                                                   <  8:81802/147‑1 (MQ=255)
                    aaTTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGc                                                                                                                                            >  8:131092/1‑138 (MQ=255)
                    aaTTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGc                                                                                                                                            <  7:131092/138‑1 (MQ=255)
                    aaTTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTaa                                                                                                                                   <  6:244034/147‑1 (MQ=255)
                    aaTTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAAc                                                                                                                                  >  2:167460/1‑148 (MQ=255)
                    aaTTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAAc                                                                                                                                  <  3:244574/148‑1 (MQ=255)
                         tATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCg                                                                                                                            >  8:98772/1‑149 (MQ=255)
                           tCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGc                                                                                                                          <  2:167880/149‑1 (MQ=255)
                             gTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCTAGGCATCGGTCATTGCATATTTTAACGAACCGGCAg                                                                                                                        <  1:223356/149‑1 (MQ=255)
                             gTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCa                                                                                                                         <  8:69191/148‑1 (MQ=255)
                             gTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAg                                                                                                                        <  3:143411/149‑1 (MQ=255)
                             gTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAg                                                                                                                        <  8:51648/149‑1 (MQ=255)
                                 gtggATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTa                                                                                                                     <  5:209935/148‑1 (MQ=255)
                                   ggATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGc                                                                                                                   >  8:271854/1‑148 (MQ=255)
                                    gATGGTTTTAATACTAAGTTCATACCGCTTTGCCCATCAGCTCGATAAGACAGCTCATTATTCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGTTAGATATTCCAGGCATTGGTCATTGCATATTTTAAGGAACCGGCAGGTAGCCa                                                                                                                 <  2:22942/149‑1 (MQ=255)
                                     aTGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCAt                                                                                                                <  1:329729/149‑1 (MQ=255)
                                        gTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTa                                                                                                             <  1:167460/149‑1 (MQ=255)
                                        gTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTa                                                                                                             <  2:72750/149‑1 (MQ=255)
                                         ttttAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTAt                                                                                                            <  8:8444/149‑1 (MQ=255)
                                         ttttAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTAt                                                                                                            <  7:186052/149‑1 (MQ=255)
                                            aaaaCCTAAGTTCATCCCGCTTTGCCCATCAGCAAGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTACCGAACCGGCAGGTAGCCATTTATGa                                                                                                          <  5:294333/147‑1 (MQ=255)
                                             aaaCCTATGTTCATACCGCTTTGCCAATCAGCACGATAAGTCAGCTCATTACTCGTCGGCGTTTCAAATCCTCGCCCTGCTGCCAGATATATATTCCAGGCATCGGTCATTGCATATTTTAACGAAc                                                                                                                              <  4:99980/127‑1 (MQ=255)
                                             aaaCCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGAt                                                                                                         >  5:307514/1‑148 (MQ=255)
                                             aaaCCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGAt                                                                                                         >  8:162659/1‑148 (MQ=255)
                                                ccTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACt                                                                                                     >  5:321050/1‑149 (MQ=255)
                                                 cTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTg                                                                                                    >  7:140062/1‑149 (MQ=255)
                                                  tAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTgg                                                                                                   >  7:297731/1‑149 (MQ=255)
                                                    aGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGc                                                                                                  <  7:162659/148‑1 (MQ=255)
                                                     gTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCAt                                                                                                >  2:242983/1‑149 (MQ=255)
                                                       tCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCACCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCa                                                                                              >  6:6024/1‑149 (MQ=255)
                                                       tCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCACCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCa                                                                                              >  6:1177/1‑149 (MQ=255)
                                                        cATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCa                                                                                              <  6:108050/148‑1 (MQ=255)
                                                            ccGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGTCATCACCGCt                                                                                         <  3:229569/149‑1 (MQ=255)
                                                                    cccATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATAtt                                                                                                                                       <  7:253258/95‑1 (MQ=255)
                                                                    cccATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATAtt                                                                                                                                       >  8:253258/1‑95 (MQ=255)
                                                                        tCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGGAGGTAGCGATTTATGATAACTGGCATCACCGCTGTCATCTCCGt                                                                              >  8:78800/1‑148 (MQ=255)
                                                                        tCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGtt                                                                             <  7:98772/149‑1 (MQ=255)
                                                                        tCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGt                                                                              >  8:320494/1‑148 (MQ=255)
                                                                                   aGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTaa                                                                  <  7:271854/149‑1 (MQ=255)
                                                                                     aCAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTACCGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACg                                                                >  2:96511/1‑149 (MQ=255)
                                                                                       aGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGt                                                               >  3:233247/1‑148 (MQ=255)
                                                                                        gCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTaa                                                             <  7:320494/149‑1 (MQ=255)
                                                                                          tCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATg                                                           >  1:29943/1‑149 (MQ=255)
                                                                                            aTTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCTGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAAGTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATg                                                           <  6:49479/147‑1 (MQ=255)
                                                                                            aTTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAAt                                                            <  6:252386/146‑1 (MQ=255)
                                                                                            aTTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAAt                                                            >  5:252386/1‑146 (MQ=255)
                                                                                             ttAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCCGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATCGTc                                                        >  8:100978/1‑149 (MQ=255)
                                                                                               aaTCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGt                                                      >  3:117098/1‑149 (MQ=255)
                                                                                                aTCTTCGGCGTTTCAAAACGTCGCCGGGCTGGCAGTTAGATATTCCAGGAATCGGTCTTTGCATATTTTAACGAACCGGCAGGTAGCCATTTTTGATAACTGGCATCACCGTTGTCATGGCCGTTACCCGGTGTAAAGTAATGGTCGtt                                                     <  4:254216/149‑1 (MQ=255)
                                                                                                aTCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGtt                                                     <  8:113669/149‑1 (MQ=255)
                                                                                                aTCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCACCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGt                                                      <  5:1177/148‑1 (MQ=255)
                                                                                                aTCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCACCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGt                                                      <  5:6024/148‑1 (MQ=255)
                                                                                                         gTTTCAAAACCTCGCCCGGCTGCCAGATAGATATGCCAGGCATCGGTCATTGCAGATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCaaa                                            <  6:237654/149‑1 (MQ=255)
                                                                                                             cAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGACATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACca                                         >  6:110868/1‑148 (MQ=255)
                                                                                                              aaaaCCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTTCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACACGGAGTAAGGTAATGGTCGTTGGAATCAAACcac                                        <  1:242983/148‑1 (MQ=255)
                                                                                                               aaaCCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCacac                                      >  6:137929/1‑149 (MQ=255)
                                                                                                                aaCCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACg                                     <  6:321050/149‑1 (MQ=255)
                                                                                                                         cGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCACTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAg                            >  7:84524/1‑149 (MQ=255)
                                                                                                                             agCCAGATAGATATTCCACGCATCGGTCATTGCAAATTATAACGAACCGGCAGGTAGCCATTTAAGATAACTCGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGAAGCGCa                        <  5:32297/148‑1 (MQ=255)
                                                                                                                                 agatagatATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGc                     >  2:17625/1‑148 (MQ=255)
                                                                                                                                  gatagataTTCCAGGCATCGGTCATTGCATATTTTAACGCACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGc                     >  2:229747/1‑147 (MQ=255)
                                                                                                                                          ttCCAGGCAGCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGGTGTAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCa            <  5:324717/148‑1 (MQ=255)
                                                                                                                                            ccAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCACTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGc          <  8:84524/148‑1 (MQ=255)
                                                                                                                                              aGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGa        >  2:210179/1‑148 (MQ=255)
                                                                                                                                               ggCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACa      <  4:117098/149‑1 (MQ=255)
                                                                                                                                                  aTCGGTCATTGCATATTTTAAAGAACCGGTAGGTAGCCATTTAAGATAACTGGCATAACCGCTGTCATAGCAGTTACACGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGtt   >  5:292473/1‑149 (MQ=255)
                                                                                                                                                   tCGTTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGttt  >  4:229255/1‑149 (MQ=255)
                                                                                                                                                   tCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGATTCAAACCACCCGGAGCTGTAGCGCACGCCAGCATACAGCGACAGttt  >  2:243019/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCGTTTTACTGCCGATCTCAATTGTATCGTTGGTGGATGGTTTTAAACCTAAGTTCATACCGCTTTGCCCATCAGCACGATAAGACAGCTCATTAATCGTCGGCGTTTCAAAACCTCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTT  >  NC_000913/1521543‑1521838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: