Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,771,897 C→A 5.1% T276K (ACA→AAA)  ydiM → putative MFS transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,771,8970CA 5.1% 258.1 / 3.5 79T276K (ACA→AAA) ydiMputative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (40/35);  new base A (2/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

GTTAGCCAGTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAATGGCT  >  NC_000913/1771749‑1772045
                                                                                                                                                    |                                                                                                                                                    
gTTAGCCAGTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCaacaac                                                                                                                                                      <  7:192040/149‑1 (MQ=255)
   aGCCAGTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCACAACAACAt                                                                                                                                                    >  3:318265/1‑148 (MQ=255)
     ccAGTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTAc                                                                                                                                                 >  8:130682/1‑149 (MQ=255)
     ccAGTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTAc                                                                                                                                                 >  7:65255/1‑149 (MQ=255)
       aGTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTAc                                                                                                                                                 <  5:27192/147‑1 (MQ=255)
       aGTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTAc                                                                                                                                                 >  6:27192/1‑147 (MQ=255)
        gTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGa                                                                                                                                              <  6:35869/149‑1 (MQ=255)
         tggctggcACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGAt                                                                                                                                             >  3:114368/1‑149 (MQ=255)
         tggctggcACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTACACTCATTCGTAATACCGTTCGCCCAACAACATTACTGAt                                                                                                                                             >  1:306457/1‑149 (MQ=255)
           gctggcACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATg                                                                                                                                            <  7:130682/148‑1 (MQ=255)
           gctggcACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGc                                                                                                                                           >  5:303898/1‑149 (MQ=255)
              ggcACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTg                                                                                                                                         >  6:247044/1‑148 (MQ=255)
               gcACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTa                                                                                                                                       <  6:303898/149‑1 (MQ=255)
                 acaGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTaca                                                                                                                                     >  8:102362/1‑149 (MQ=255)
                    gTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCt                                                                                                                                  <  1:57365/149‑1 (MQ=255)
                    gTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAAAATTACTGATGCTGTACACCt                                                                                                                                  >  8:220714/1‑149 (MQ=255)
                    gTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAAAATTACTGATGCTGTACACCt                                                                                                                                  >  8:215675/1‑149 (MQ=255)
                    gTACGGAAAATTTGTTGCAGGCATGTAATACACTATTTAGATGAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCt                                                                                                                                  <  8:322532/149‑1 (MQ=255)
                       cGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCCGTCATTCGGAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTa                                                                                                                               >  6:3081/1‑149 (MQ=255)
                       cGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACTCCttt                                                                                                                                <  4:114368/148‑1 (MQ=255)
                        ggACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCCCTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTAt                                                                                                                              >  5:26839/1‑149 (MQ=255)
                         gACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTAtc                                                                                                                             >  5:38282/1‑149 (MQ=255)
                            aaTTTGTTGCAGGCATGGAATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCa                                                                                                                          <  4:38943/149‑1 (MQ=255)
                              tttGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCAtt                                                                                                                        >  2:320256/1‑149 (MQ=255)
                              tttGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCAtt                                                                                                                        >  1:151100/1‑149 (MQ=255)
                                    gCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTg                                                                                                                   <  6:26839/148‑1 (MQ=255)
                                     cAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGct                                                                                                                 <  5:325956/149‑1 (MQ=255)
                                     cAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGct                                                                                                                 <  6:88229/149‑1 (MQ=255)
                                        gCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACACATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTg                                                                                                              >  8:123913/1‑149 (MQ=255)
                                               aTACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTAcc                                                                                                        <  2:91189/148‑1 (MQ=255)
                                               aTACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCCTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCg                                                                                                       <  7:66723/149‑1 (MQ=255)
                                                     tATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTctgcc                                                                                                 >  2:79538/1‑149 (MQ=255)
                                                       tGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTctgcct                                                                                                <  2:306457/148‑1 (MQ=255)
                                                           gatcttactactCATTATGTTCACCGTGGGTTCGCTGGTTTGTGTATTTATTTCCGCTCCACTCATTAGTAATACCGTTCGCCCAACAACATTACTGATTCTGTACACCTTTATCTCATTTATTGCTCTGTTTTCCGTCTGCCTGCAt                                                                                            <  8:287659/142‑1 (MQ=255)
                                                             tCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAAAATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATccc                                                                                         <  7:215675/149‑1 (MQ=255)
                                                             tCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAAAATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATccc                                                                                         <  7:220714/149‑1 (MQ=255)
                                                                aaCTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCcaca                                                                                      >  7:11549/1‑149 (MQ=255)
                                                                 actactCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACAt                                                                                     >  7:60110/1‑149 (MQ=255)
                                                                   tactCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACAttt                                                                                   >  3:76446/1‑149 (MQ=255)
                                                                   tactCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCAACTGATTCGTAGTACCGTTCGCCCAACACCATTTCTGATGATGTACACCTTTATCTCATTTATTGATCTGTTTACCGTCTGCCTGCATCCAACAttt                                                                                   >  4:130664/1‑149 (MQ=255)
                                                                    actCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACAttt                                                                                   >  7:338523/1‑148 (MQ=255)
                                                                        aGTGTCTACCCCGTGGGTTCGCTGGTTTGTGTATTTATTACCGCTCCGCTCGTTCGTAATACCGTTCGCGCAACAACATTACTGATGCGGGACACCTTTATCTCAGTTATTGCGCTGTTTGCCGGCTGCCTGCGTCCGACATTTTAtgt                                                                              >  4:51908/1‑149 (MQ=255)
                                                                         gTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTAtgtg                                                                             <  7:102362/149‑1 (MQ=255)
                                                                         gTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTAAACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTAATTGCTATGTTTACCGTCTGCCTGCATCCCACATTTTAtgtg                                                                             >  2:152167/1‑149 (MQ=255)
                                                                          tATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATgtgg                                                                            >  6:29192/1‑149 (MQ=255)
                                                                            tCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATgtggt                                                                           <  1:246457/148‑1 (MQ=255)
                                                                              tACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGa                                                                         >  1:158221/1‑148 (MQ=255)
                                                                                cacCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGataa                                                                      >  2:114983/1‑149 (MQ=255)
                                                                                 acCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGataa                                                                      >  5:293263/1‑148 (MQ=255)
                                                                                   cGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCa                                                                                                                          <  8:114458/94‑1 (MQ=255)
                                                                                   cGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCa                                                                                                                          >  7:114458/1‑94 (MQ=255)
                                                                                   cGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAatat                                                                   <  5:146377/149‑1 (MQ=255)
                                                                                     tGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATAttt                                                                 <  1:79538/149‑1 (MQ=255)
                                                                                         ttCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCtt                                                             >  7:333941/1‑149 (MQ=255)
                                                                                           cGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCttt                                                            <  4:76446/148‑1 (MQ=255)
                                                                                                ctTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTc                                                        <  1:29463/147‑1 (MQ=255)
                                                                                                ctTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTc                                                        <  8:338523/147‑1 (MQ=255)
                                                                                                      gtATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTAcc                                                                                                        >  3:82719/1‑93 (MQ=255)
                                                                                                      gtATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTAcc                                                                                                        <  4:82719/93‑1 (MQ=255)
                                                                                                      gtATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTATTGCTCTGTTTACCGTctgcct                                                                                                >  4:175943/1‑100 (MQ=255)
                                                                                                      gtATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTATTGCTCTGTTTACCGTctgcct                                                                                                <  3:175943/100‑1 (MQ=255)
                                                                                                        atttattACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTct                                                                                                    >  4:143253/1‑95 (MQ=255)
                                                                                                        atttattACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTct                                                                                                    <  3:143253/95‑1 (MQ=255)
                                                                                                         tttattACCGCTCCACTCATTCGTACTCCCGTTCTCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTCTTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGGGATAATATTTGCTTTTGTCATTGGTTTTAc                                             <  8:307591/149‑1 (MQ=255)
                                                                                                         tttattACCGCTCCACTCATTCGTAATACCGTGCGCCCAACAACATTACTGATGCAGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATATTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTAc                                             <  6:211557/149‑1 (MQ=255)
                                                                                                               aCCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGATGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTctg                                        <  2:70531/148‑1 (MQ=255)
                                                                                                                ccGCTCCACTCATTCGTAATACCGTTCGCTCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTAcc                                                                                                        >  1:328097/1‑83 (MQ=255)
                                                                                                                ccGCTCCACTCATTCGTAATACCGTTCGCTCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTAcc                                                                                                        <  2:328097/83‑1 (MQ=255)
                                                                                                                   cTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACctccagca                                     >  5:275389/1‑142 (MQ=255)
                                                                                                                   cTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGa                                   <  1:320256/149‑1 (MQ=255)
                                                                                                                        cTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGgtgt                              >  4:85908/1‑149 (MQ=255)
                                                                                                                         tCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGtgtt                             >  7:204117/1‑149 (MQ=255)
                                                                                                                         tCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGtgtt                             >  1:179733/1‑149 (MQ=255)
                                                                                                                           aTTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGtgtt                             <  5:247044/147‑1 (MQ=255)
                                                                                                                           aTTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGtgtt                             <  6:38282/147‑1 (MQ=255)
                                                                                                                               gTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCa                        >  2:166459/1‑148 (MQ=255)
                                                                                                                                     ccGTTCGCCCAACAACATAACTGATGCTGTACACCTTTCTCTCATTTATTGCTCTGTTTACCGTCTGCCTCCAGCCCACAATTTATGTGTTGATAATATTAGCTTTTGTCATTGGTATTACCTCTGCTGGAGGTGTTGTTCAAATTgg                  <  3:130664/148‑1 (MQ=255)
                                                                                                                                      cGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGtttt                                               <  4:256964/118‑1 (MQ=255)
                                                                                                                                      cGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGtttt                                               >  3:256964/1‑118 (MQ=255)
                                                                                                                                             ccAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGt          >  2:334231/1‑148 (MQ=255)
                                                                                                                                               aacaacATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTaa       <  8:333941/149‑1 (MQ=255)
                                                                                                                                                acaacaTTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAAt      <  7:123913/149‑1 (MQ=255)
                                                                                                                                                acaacaTTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAAt      <  5:3081/149‑1 (MQ=255)
                                                                                                                                                acaacaTTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAAt      <  2:151100/149‑1 (MQ=255)
                                                                                                                                                acaacaTTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAAt      >  2:182831/1‑149 (MQ=255)
                                                                                                                                                  aacaTTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAATgg    >  7:313690/1‑149 (MQ=255)
                                                                                                                                                   acaTTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAATGGc   <  3:51908/149‑1 (MQ=255)
                                                                                                                                                    caTTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAATGGCt  >  7:222862/1‑149 (MQ=255)
                                                                                                                                                    caTTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAATGGCt  <  8:60110/149‑1 (MQ=255)
                                                                                                                                                    caTTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGGCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGa             <  2:358217/138‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTAGCCAGTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAATGGCT  >  NC_000913/1771749‑1772045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: